{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:47:48Z","timestamp":1786121268939,"version":"build-2736575974"},"reference-count":37,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013805","name":"Shanxi Agricultural University","doi-asserted-by":"publisher","award":["2024BQ14"],"award-info":[{"award-number":["2024BQ14"]}],"id":[{"id":"10.13039\/501100013805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013805","name":"Shanxi Agricultural University","doi-asserted-by":"publisher","award":["S202510113016"],"award-info":[{"award-number":["S202510113016"]}],"id":[{"id":"10.13039\/501100013805","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2024BQ14"],"award-info":[{"award-number":["2024BQ14"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"publisher"}]},{"award":["S202510113016"],"award-info":[{"award-number":["S202510113016"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"publisher"}]},{"name":"The education department of Shanxi Province","award":["SXBYKY2024090"],"award-info":[{"award-number":["SXBYKY2024090"]}]},{"award":["SXBYKY2024090"],"award-info":[{"award-number":["SXBYKY2024090"]}],"id":[{"id":"https:\/\/ror.org\/00jb7xy46","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Rabbit Industry Technology System Special Fund","award":["CARS-43-B-3"],"award-info":[{"award-number":["CARS-43-B-3"]}]},{"name":"Shanxi Province Postdoctoral Special Financial Assistance Program","award":["6K265520004440"],"award-info":[{"award-number":["6K265520004440"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Aflatoxin B1 (AFB1) is a prevalent mycotoxin contaminating food and environmental matrices that can induce hepatic injury, thereby posing a substantial threat to livestock production. Portulaca oleracea L. polysaccharide (POP) possesses diverse biological activities, including antioxidant and anti-inflammatory properties. However, the protective effects of POP against AFB1-induced hepatic injury have not been fully elucidated. In the present study, a rabbit model of AFB1-induced hepatic injury was established to investigate the hepatoprotective mechanisms of POP. Histopathological examination and liver function assessments demonstrated that POP markedly ameliorated hepatic histopathological alterations and decreased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Molecular analyses further demonstrated that POP attenuated AFB1-induced oxidative stress and hepatocyte apoptosis, effects that were associated with activation of the PI3K\/AKT signaling pathway and suppression of the mitochondria-mediated apoptotic pathway. Furthermore, microbiome profiling and targeted short-chain fatty acid (SCFA) metabolomics analyses indicated that POP supplementation contributed to the preservation of intestinal barrier integrity and intestinal microbiota stability, concurrent with the attenuation of AFB1-induced intestinal and hepatic injury and elevated SCFA production by intestinal microbes. Overall, POP exerts prominent preventive protective effects against AFB1-induced liver injury, which may be closely associated with alterations in intestinal microbiota composition and microbial short-chain fatty acid metabolism, as well as the activation of the PI3K\/AKT signaling pathway. These findings provide novel insights into the mechanisms underlying AFB1-induced hepatic injury.<\/jats:p>","DOI":"10.3390\/biology15151311","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:16:29Z","timestamp":1785935789000},"page":"1311","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Portulaca oleracea L. Polysaccharide Alleviates AFB1-Induced Liver Injury in New Zealand Rabbits via the Gut\u2013Liver Axis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1465-9207","authenticated-orcid":false,"given":"Chunxiao","family":"Liu","sequence":"first","affiliation":[{"name":"Shanxi Key Laboratory of Animal Nutrition and Feed Development, College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruyi","family":"Hu","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Animal Nutrition and Feed Development, College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Meng","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Animal Nutrition and Feed Development, College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Niu","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Animal Nutrition and Feed Development, College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Cao","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Animal Nutrition and Feed Development, College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Animal Nutrition and Feed Development, College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.fct.2017.06.034","article-title":"Aflatoxin B1-induced epigenetic alterations: An overview","volume":"109","author":"Dai","year":"2017","journal-title":"Food Chem. 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