{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T21:22:38Z","timestamp":1793136158810,"version":"4.5.0"},"reference-count":83,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32101788"],"award-info":[{"award-number":["32101788"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32101788"],"award-info":[{"award-number":["32101788"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Doctoral Research Start-up Foundation and Experimental Teaching Reform Project of Liaocheng University","award":["318052021"],"award-info":[{"award-number":["318052021"]}]},{"name":"Doctoral Research Start-up Foundation and Experimental Teaching Reform Project of Liaocheng University","award":["3111625054"],"award-info":[{"award-number":["3111625054"]}]},{"name":"Higher Educational Program of China for Cultivation of Young Innovative Talents","award":["202510447013"],"award-info":[{"award-number":["202510447013"]}]},{"name":"Higher Educational Program of China for Cultivation of Young Innovative Talents","award":["202510447014"],"award-info":[{"award-number":["202510447014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Plant A\/T-rich sequence and zinc-binding protein (PLATZ) transcription factors are pivotal regulators of plant growth, development, and abiotic stress responses. Although PLATZ genes have been extensively studied and functionally characterized in various plant species, little information is available regarding these genes in the legume model plant Medicago truncatula. In this study, a total of 16 PLATZ genes (designated MtPLATZ1 to MtPLATZ16) were identified from the M. truncatula genome and distributed across six of the eight chromosomes. Subcellular localization prediction analysis indicated that all MtPLATZ proteins are localized in the nucleus, with eight representative members experimentally validated by transient expression assays. Collinearity analysis indicated that five segmental duplication pairs were the primary driver of MtPLATZ gene expansion. Based on phylogenetic analysis, these genes were classified into four groups, comprising 6, 2, 4, and 4 genes, respectively. Gene structure analysis demonstrated that these genes possess three or four exons. Most MtPLATZ genes were found to contain at least one ABRE cis-element in their promoter regions, and a few also harbored LTR, CAT-box, ARE, and DRE cis-elements. Expression analysis revealed that MtPLATZ genes exhibit distinct expression patterns across different tissues, suggesting that they may be differentially regulated by abiotic stresses in a tissue-specific manner. Under PEG-induced osmotic stress, acute NaCl shock, acute heat shock, and acute cold shock, the expression levels of these eight genes exhibited significant changes, particularly MtPLATZ2, MtPLATZ6, and MtPLATZ9, indicating that MtPLATZ gene family members may play a broad role in abiotic stress responses. In summary, these findings provide valuable insights for future functional exploration of MtPLATZ genes and contribute to a deeper understanding of their roles in stress responses in M. truncatula.<\/jats:p>","DOI":"10.3390\/biology15151318","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T08:08:02Z","timestamp":1786003682000},"page":"1318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome-Wide Characterization of the PLATZ Gene Family in Medicago truncatula and Their Expression Profiles in Response to Abiotic Stress"],"prefix":"10.3390","volume":"15","author":[{"given":"Wen","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5287-2464","authenticated-orcid":false,"given":"Shuangjin","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6460-0267","authenticated-orcid":false,"given":"Xinyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxi","family":"Zang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongwang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1126\/science.290.5499.2105","article-title":"Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes","volume":"290","author":"Riechmann","year":"2000","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102136","DOI":"10.1016\/j.pbi.2021.102136","article-title":"Plant transcription factors\u2014Being in the right place with the right company","volume":"65","author":"Strader","year":"2022","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zuo, Z.F., Lee, H.Y., and Kang, H.G. (2023). Basic helix-loop-helix transcription factors: Regulators for plant growth development and abiotic stress responses. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24021419"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Li, Y., Gong, K., Wang, X., Sun, Z., and Ding, F. (2025). Heat shock transcription factors as central integrators of plant stress responses: From thermotolerance to multi-stress resilience. Biology, 14.","DOI":"10.3390\/biology14121800"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0959-440X(00)00167-6","article-title":"Zinc finger proteins: New insights into structural and functional diversity","volume":"11","author":"Laity","year":"2001","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4097","DOI":"10.1093\/nar\/29.20.4097","article-title":"A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A\/T-rich DNA sequences","volume":"29","author":"Nagano","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1105\/tpc.17.00576","article-title":"The maize imprinted gene Floury3 encodes a PLATZ protein required for tRNA and 5S rRNA transcription through interaction with RNA polymerase III","volume":"29","author":"Li","year":"2017","journal-title":"Plant Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1080\/13102818.2022.2155570","article-title":"Systematic identification and characterization of the soybean (Glycine max) B-box transcription factor family","volume":"37","author":"Sui","year":"2023","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"130603","DOI":"10.1016\/j.ijbiomac.2024.130603","article-title":"Genome-wide identification, evolution of DNA methyltransferases and their expression under salinity stress in Larimichthys crocea","volume":"264","author":"Zhang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Basharat, S., Liu, P., and Waseem, M. (2025). PLATZ transcription factors gene family in Brassica napus: Genome-wide identification and expression analysis to abiotic stresses. BMC Genom., 27.","DOI":"10.1186\/s12864-025-12434-8"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Feng, X., Zhu, G., Meng, Q., Zeng, J., He, X., and Liu, W. (2024). Comprehensive analysis of PLATZ family genes and their responses to abiotic stresses in barley. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-05690-5"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wang, X., Huang, Y., Yang, N., Wang, X., Wang, Y., Ma, W., and Zhang, H. (2025). Genome-wide identification of CaPLATZ family members in pepper and their expression profiles in response to drought stress. Genes, 16.","DOI":"10.3390\/genes16060632"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Han, X., Rong, H., Tian, Y., Qu, Y., Xu, M., and Xu, L.A. (2022). Genome-wide identification of PLATZ transcription factors in Ginkgo biloba L. and their expression characteristics during seed development. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.946194"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/nph.15291","article-title":"ORESARA15, a PLATZ transcription factor, mediates leaf growth and senescence in Arabidopsis","volume":"220","author":"Kim","year":"2018","journal-title":"New Phytol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1104\/pp.18.01574","article-title":"The PLATZ transcription factor GL6 affects grain length and number in rice","volume":"180","author":"Wang","year":"2019","journal-title":"Plant Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Yang, T., Xu, X.T., Tang, L.J., Wei, W.T., Zhao, Y.Y., Liu, J.X., Yao, X.F., Zhao, H., Liu, C.M., and Bai, A.N. (2025). Genome-wide study of plant-specific PLATZ transcription factors and functional analysis of OsPLATZ1 in regulating caryopsis development of rice (Oryza sativa L.). Plants, 14.","DOI":"10.3390\/plants14020151"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Wang, J., Ji, C., Li, Q., Zhou, Y., and Wu, Y. (2018). Genome-wide analysis of the plant-specific PLATZ proteins in maize and identification of their general role in interaction with RNA polymerase III complex. BMC Plant Biol., 18.","DOI":"10.1186\/s12870-018-1443-x"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Li, X., He, F., Zhao, G., Li, M., Long, R., Kang, J., Yang, Q., and Chen, L. (2023). Genome-wide identification and phylogenetic and expression analyses of the PLATZ gene family in Medicago sativa L. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032388"},{"key":"ref_19","first-page":"221","article-title":"Identification, expression profiling, and natural allelic variation analysis of the PLATZ gene family in sorghum","volume":"42","author":"Shi","year":"2026","journal-title":"Chin. J. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Fan, B., Ren, M., Chen, G., Zhou, X., Cheng, G., Yang, J., and Sun, H. (2025). Exploring the roles of the plant AT-rich sequence and Zinc-Binding (PLATZ) gene family in tomato (Solanum lycopersicum L.) under abiotic stresses. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26041682"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wang, M., Guan, Z., Wu, S., Zhang, J., Lin, C., Sun, Y., Shen, M., and Zhang, C. (2025). Genome-wide identification and salt stress-responsive expression analysis of the GmPLATZ gene family in soybean (Glycine max L.). Plants, 14.","DOI":"10.3390\/plants14132004"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Fu, Y., Cheng, M., Li, M., Guo, X., Wu, Y., and Wang, J. (2020). Identification and characterization of PLATZ transcription factors in wheat. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21238934"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"112400","DOI":"10.1016\/j.plantsci.2025.112400","article-title":"PLATZ transcription factors and their emerging roles in plant responses to environmental stresses","volume":"352","author":"Zhang","year":"2025","journal-title":"Plant Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s10725-020-00687-0","article-title":"Transcription factor ZmPLATZ2 positively regulate the starch synthesis in maize","volume":"93","author":"Li","year":"2021","journal-title":"Plant Growth Regul."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhang, S., Yang, R., Huo, Y., Liu, S., Yang, G., Huang, J., Zheng, C., and Wu, C. (2018). Expression of cotton PLATZ1 in transgenic Arabidopsis reduces sensitivity to osmotic and salt stress for germination and seedling establishment associated with modification of the abscisic acid, gibberellin, and ethylene signalling pathways. BMC Plant Biol., 18.","DOI":"10.1186\/s12870-018-1416-0"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Liu, T., Xue, Y., Ding, X., Zhang, Y., Li, Z., Zhang, Z., Yang, Z., Zhu, J., and Liu, R. (2025). Overexpression of cotton PLATZ transcription factors GhPLATZ01 and GhPLATZ15 improves the drought resistance of tomato. BMC Plant Biol., 25.","DOI":"10.1186\/s12870-025-07428-3"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1111\/jipb.12851","article-title":"The rice PLATZ protein SHORT GRAIN6 determines grain size by regulating spikelet hull cell division","volume":"62","author":"Zhou","year":"2020","journal-title":"J. Integr. Plant Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.plaphy.2022.07.004","article-title":"PhePLATZ1, a PLATZ transcription factor in moso bamboo (Phyllostachys edulis), improves drought resistance of transgenic Arabidopsis thaliana","volume":"186","author":"Zhang","year":"2022","journal-title":"Plant Physiol. Biochem."},{"key":"ref_29","first-page":"E5232","article-title":"Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana","volume":"113","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"110821","DOI":"10.1016\/j.plantsci.2021.110821","article-title":"ABA-INDUCED expression 1 is involved in ABA-inhibited primary root elongation via modulating ROS homeostasis in Arabidopsis","volume":"304","author":"Dong","year":"2021","journal-title":"Plant Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.jare.2020.10.004","article-title":"A multilayered cross-species analysis of GRAS transcription factors uncovered their functional networks in plant adaptation to the environment","volume":"29","author":"Liu","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/tpj.16235","article-title":"Regulation of drought tolerance in Arabidopsis involves the PLATZ4-mediated transcriptional repression of plasma membrane aquaporin PIP2; 8","volume":"115","author":"Liu","year":"2023","journal-title":"Plant J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1038\/s41586-019-1819-6","article-title":"RGF1 controls root meristem size through ROS signalling","volume":"577","author":"Yamada","year":"2020","journal-title":"Nature"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sm\u00fdkal, P., von Wettberg, E.J.B., and McPhee, K. (2020). Legume genetics and biology: From Mendel\u2019s pea to legume genomics. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21093336"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.pbi.2008.11.005","article-title":"Translating Medicago truncatula genomics to crop legumes","volume":"12","author":"Young","year":"2009","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s12298-017-0421-3","article-title":"Genome-wide analysis and expression patterns of the NAC transcription factor family in Medicago truncatula","volume":"23","author":"Ling","year":"2017","journal-title":"Physiol. Mol. Biol. Plants"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1590\/1678-4685-gmb-2018-0235","article-title":"Genome-wide identification and characterization of R2R3-MYB genes in Medicago truncatula","volume":"42","author":"Li","year":"2019","journal-title":"Genet Mol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3624","DOI":"10.1016\/j.ygeno.2020.03.008","article-title":"Genome-wide identification and expression profiling of HD-ZIP gene family in Medicago truncatula","volume":"112","author":"Li","year":"2020","journal-title":"Genomics"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Jiao, Z., Wang, L., Du, H., Wang, Y., Wang, W., Liu, J., Huang, J., Huang, W., and Ge, L. (2020). Genome-wide study of C2H2 zinc finger gene family in Medicago truncatula. BMC Plant Biol., 20.","DOI":"10.1186\/s12870-020-02619-6"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1016\/j.molp.2025.07.016","article-title":"Two complete telomere-to-telomere genome assemblies of Medicago reveal the landscape and evolution of its centromeres","volume":"18","author":"Shen","year":"2025","journal-title":"Mol. Plant."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","article-title":"TBtools: An integrative toolkit developed for interactive analyses of big biological data","volume":"13","author":"Chen","year":"2020","journal-title":"Mol. Plant."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1093\/pcp\/pcab110","article-title":"MtExpress, a comprehensive and curated RNAseq-based gene expression atlas for the model legume Medicago truncatula","volume":"62","author":"Carrere","year":"2021","journal-title":"Plant Cell Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"D384","DOI":"10.1093\/nar\/gkac1096","article-title":"The conserved domain database in 2023","volume":"51","author":"Wang","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Li, Z., Lv, S., Liu, Y., Cao, M., Zhang, H., and Hao, Q. (2025). Transcriptome and metabolome analyses reveal the accumulation mechanism of carbohydrates during paeonia ostii seed development. Biomolecules, 16.","DOI":"10.3390\/biom16010017"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2367097","DOI":"10.1080\/13102818.2024.2367097","article-title":"Transcriptome analysis unravels differential genes involved in essential oil content in callus and tissue culture seedlings of Lavandula angustifolia","volume":"38","author":"Cheng","year":"2024","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2266529","DOI":"10.1080\/13102818.2023.2266529","article-title":"Overexpression of GhSWEET42, a SWEET-like gene from cotton, enhances the oil content and seed size","volume":"37","author":"Wang","year":"2023","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Macovei, A., Pagano, A., Cappuccio, M., Gallotti, L., Dondi, D., De Sousa Araujo, S., Fevereiro, P., and Balestrazzi, A. (2019). A snapshot of the trehalose pathway during seed imbibition in Medicago truncatula reveals temporal- and stress-dependent shifts in gene expression patterns associated with metabolite changes. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.01590"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1080\/07352689.2024.2388013","article-title":"Integration of the plant-specific PLATZ transcription factors into gene regulatory networks controlling developmental processes","volume":"43","author":"Baucher","year":"2024","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1110\/ps.041009005","article-title":"Understanding the relationship between the primary structure of proteins and its propensity to be soluble on overexpression in Escherichia coli","volume":"14","author":"Balaji","year":"2005","journal-title":"Protein Sci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Cannon, S.B., Mitra, A., Baumgarten, A., Young, N.D., and May, G. (2004). The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana. BMC Plant Biol., 4.","DOI":"10.1186\/1471-2229-4-10"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Yu, J., Wang, J., Lin, W., Li, S., Li, H., Zhou, J., Ni, P., Dong, W., Hu, S., and Zeng, C. (2005). The genomes of Oryza sativa: A history of duplications. PLoS Biol., 3.","DOI":"10.1371\/journal.pbio.0030038"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2010-11-3-r25","article-title":"A scaling normalization method for differential expression analysis of RNA-seq data","volume":"11","author":"Robinson","year":"2010","journal-title":"Genome Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"17570","DOI":"10.1074\/jbc.RA119.010219","article-title":"Proteomic analysis of affinity-purified 26S proteasomes identifies a suite of assembly chaperones in Arabidopsis","volume":"294","author":"Gemperline","year":"2019","journal-title":"J. Biol. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"381","DOI":"10.5483\/BMBRep.2024-0101","article-title":"Nuclear structures and their emerging roles in cell differentiation and development","volume":"57","author":"Cha","year":"2024","journal-title":"BMB Rep."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Wai, A.H., Rahman, M.M., Waseem, M., Cho, L.H., Naing, A.H., Jeon, J.S., Lee, D.J., Kim, C.K., and Chung, M.Y. (2022). Comprehensive genome-wide analysis and expression pattern profiling of PLATZ gene family members in Solanum lycopersicum L. under multiple abiotic stresses. Plants, 11.","DOI":"10.3390\/plants11223112"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Sun, Y., Liu, Y., Liang, J., Luo, J., Yang, F., Feng, P., Wang, H., Guo, B., Ma, F., and Zhao, T. (2023). Identification of PLATZ genes in Malus and expression characteristics of MdPLATZs in response to drought and ABA stresses. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1109784"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"28","DOI":"10.9787\/PBB.2020.8.1.28","article-title":"Genome-wide characterization and expression profiling of plant-specific PLATZ transcription factor family genes in Brassica rapa L.","volume":"8","author":"Khan","year":"2020","journal-title":"Plant Breed. Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"125959","DOI":"10.1016\/j.ijbiomac.2023.125959","article-title":"Whole-genome identification and multiple abiotic stresses expression pattern profiling analysis of PLATZ transcription factor family members in Pecan (Carya illinoensis)","volume":"248","author":"Zhang","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Saccone, S., Brancato, D., Bruno, F., Coniglio, E., Sturiale, V., and Federico, C. (2025). Origin and evolution of genes in eukaryotes: Mechanisms, dynamics, and functional implications. Genes, 16.","DOI":"10.3390\/genes16060702"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e5023","DOI":"10.21769\/BioProtoc.5023","article-title":"Phylogenomics of plant NLR immune receptors to identify functionally conserved sequence motifs","volume":"14","author":"Sakai","year":"2024","journal-title":"Bio. Protoc."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s11032-024-01522-4","article-title":"The pan-NLRome analysis based on 23 genomes reveals the diversity of NLRs in Brassica napus","volume":"44","author":"Ning","year":"2024","journal-title":"Mol. Breed."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Nian, L., Liu, X., Yang, Y., Zhu, X., Yi, X., and Haider, F.U. (2021). Genome-wide identification, phylogenetic, and expression analysis under abiotic stress conditions of LIM gene family in Medicago sativa L. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0252213"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Gao, C., Li, Z., Zhang, H., Li, C., Sun, H., Li, S., Ma, N., Qi, X., Cui, Y., and Yang, P. (2024). Genome-wide identification and characterization of the GASA gene family in Medicago truncatula, and expression patterns under abiotic stress and hormone treatments. Plants, 13.","DOI":"10.3390\/plants13172364"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1007\/s00239-003-2564-9","article-title":"Dating the monocot-dicot divergence and the origin of core eudicots using whole chloroplast genomes","volume":"58","author":"Chaw","year":"2004","journal-title":"J. Mol. Evol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"136652","DOI":"10.1016\/j.ijbiomac.2024.136652","article-title":"MYB transcription factors in plants: A comprehensive review of their discovery, structure, classification, functional diversity and regulatory mechanism","volume":"282","author":"Wu","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1093\/jxb\/eraa259","article-title":"PLATZ2 negatively regulates salt tolerance in Arabidopsis seedlings by directly suppressing the expression of the CBL4\/SOS3 and CBL10\/SCaBP8 genes","volume":"71","author":"Liu","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Gu, F., Ren, Y., Manzoor, M.A., Wang, T., Huang, R., Chen, N., Song, C., and Zhang, Y. (2024). Plant AT-rich protein and zinc-binding protein (PLATZ) family in Dendrobium huoshanense: Identification, evolution and expression analysis. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-06009-0"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Jiang, M., and Chu, Z. (2018). Comparative analysis of plant MKK gene family reveals novel expansion mechanism of the members and sheds new light on functional conservation. BMC Genom., 19.","DOI":"10.1186\/s12864-018-4793-8"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/nature10625","article-title":"The Medicago genome provides insight into the evolution of rhizobial symbioses","volume":"480","author":"Young","year":"2011","journal-title":"Nature"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1111\/tpj.12089","article-title":"Gene family evolution in green plants with emphasis on the origination and evolution of Arabidopsis thaliana genes","volume":"73","author":"Guo","year":"2013","journal-title":"Plant J."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Yu, Z., Zheng, C., Albert, V.A., and Sankoff, D. (2020). Excision Dominates Pseudogenization During Fractionation After Whole Genome Duplication and in Gene Loss After Speciation in Plants. Front. Genet., 11.","DOI":"10.3389\/fgene.2020.603056"},{"key":"ref_73","first-page":"277","article-title":"Intron-mediated regulation of gene expression","volume":"326","author":"Rose","year":"2008","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_74","first-page":"109","article-title":"Identification and validation of promoters and cis-acting regulatory elements","volume":"217\u2013218","author":"Finer","year":"2014","journal-title":"Plant Sci."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1007\/s00299-013-1418-1","article-title":"ABA signaling in stress-response and seed development","volume":"32","author":"Nakashima","year":"2013","journal-title":"Plant Cell Rep."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1111\/nph.18159","article-title":"Auxin response factors are keys to the many auxin doors","volume":"235","author":"Boubekeur","year":"2022","journal-title":"New Phytol."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Ali, S., and Moon, Y.S. (2026). Molecular mechanisms of plant stress tolerance: From stress perception to phytohormonal crosstalk and transcriptional regulation. Curr. Issues Mol. Biol., 48.","DOI":"10.3390\/cimb48050474"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Wang, M., Zhang, S., and Ding, F. (2020). Melatonin mitigates chilling-induced oxidative stress and photosynthesis inhibition in tomato plants. Antioxidants, 9.","DOI":"10.3390\/antiox9030218"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e70097","DOI":"10.1111\/jpi.70097","article-title":"Exogenous melatonin enhances the cold resistance of pepper (Capsicum annuum L. var. cerasiforme Bailey) by Promoting the synthesis of long-chain alkanes in the epicuticular wax","volume":"77","author":"Wu","year":"2025","journal-title":"J. Pineal Res."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Jia, X., Pang, X., Xu, M., Wang, C., Wei, H., Liu, J., Zhang, H., Li, D., Xu, X., and Zhao, T. (2025). The overexpression of SlPLATZ17 can increase the tolerance of tomatoes to drought and salt stress. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26051836"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/nph.18372","article-title":"Histone acetyltransferase TaHAG1 interacts with TaPLATZ5 to activate TaPAD4 expression and positively contributes to powdery mildew resistance in wheat","volume":"236","author":"Song","year":"2022","journal-title":"New Phytol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1104\/pp.113.214049","article-title":"EBE, an AP2\/ERF transcription factor highly expressed in proliferating cells, affects shoot architecture in Arabidopsis","volume":"162","author":"Mehrnia","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"R130","DOI":"10.1186\/gb-2008-9-8-r130","article-title":"Global transcriptome analysis reveals circadian regulation of key pathways in plant growth and development","volume":"9","author":"Covington","year":"2008","journal-title":"Genome Biol."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/15\/1318\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T08:41:41Z","timestamp":1786005701000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/15\/1318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,6]]},"references-count":83,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["biology15151318"],"URL":"https:\/\/doi.org\/10.3390\/biology15151318","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,6]]}}}