{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:29:05Z","timestamp":1786699745019,"version":"build-2736575974"},"reference-count":66,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003624","name":"Ministry of Agriculture, Food and Rural Affairs","doi-asserted-by":"publisher","award":["RS-2024-00403514"],"award-info":[{"award-number":["RS-2024-00403514"]}],"id":[{"id":"10.13039\/501100003624","id-type":"DOI","asserted-by":"publisher"}]},{"award":["RS-2024-00403514"],"award-info":[{"award-number":["RS-2024-00403514"]}],"id":[{"id":"https:\/\/ror.org\/009g8rq41","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>As LEDs are increasingly used in mushroom production, understanding the blue-light response characteristics of the pathogenic fungus Trichoderma harzianum is important for mushroom cultivation management. In this study, T. harzianum was cultured under blue LED conditions (460 nm), with darkness as the control, and its growth, morphology, and transcriptomic responses were compared. Blue LED exposure reduced colony diameter by 21.6% and 25.2% on days 1 and 2 of cultivation, respectively, and induced sporulation on day 3. RNA-seq Trinity gene-level features were quantified, and 23,086 and 34,839 genes showed higher and lower expression under blue LED illumination, respectively. Gene Ontology (GO) analysis of candidates selected using the criteria TPM \u2265 15 and |log2FC| \u2265 1 showed that the candidates with increased expression blue LED illumination were associated with transport and xenobiotic transport, whereas the candidates with decreased expression were associated with filamentous growth, cell wall polysaccharide biosynthetic process, and UDP-glucosyltransferase activity. Among the highly responsive candidates selected using the criteria TPM \u2265 15 and |log2FC| \u2265 3, carbohydrate-active enzyme (CA-Zyme)-related genes were identified, including glycosyltransferase family (GT) 8, GT35, glycoside hydrolase family (GH) 16, GH18, GH76, and multicopper oxidase. In contrast, the candidates with decreased expression included GT2, GT20, GT35, carbohydrate esterase family 5, and GMC oxidoreductase. RT-qPCR analysis of the selected candidate genes supported the RNA-seq-based expression trends. In addition, in the RNA-seq dataset, the expression levels of the blue light response-related genes BLR-1, BLR-2, and ENV1 increased by 2.8-, 1.9-, and 4.2-fold, respectively. These findings provide phenotypic and molecular candidates for future functional studies of the blue-light response in T. harzianum.<\/jats:p>","DOI":"10.3390\/biology15161390","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:08:29Z","timestamp":1786694909000},"page":"1390","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Blue LED-Associated Morphological Changes and Gene Expression Responses in Trichoderma harzianum"],"prefix":"10.3390","volume":"15","author":[{"given":"Dong-Ryeol","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Seo","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3313-6532","authenticated-orcid":false,"given":"Youn Jin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6654-7939","authenticated-orcid":false,"given":"Myoung-Jun","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/s41579-018-0109-x","article-title":"Light sensing and responses in fungi","volume":"17","author":"Yu","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e00149-22","DOI":"10.1128\/mmbr.00149-22","article-title":"How fungi see the world: Fungal photoreceptors and their role in the regulation of fungal biology","volume":"89","author":"Corrochano","year":"2025","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Bayram, \u00d6.S., and Bayram, \u00d6. (2023). An anatomy of fungal eye: Fungal photoreceptors and signalling mechanisms. J. Fungi, 9.","DOI":"10.3390\/jof9050591"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"R1134","DOI":"10.1016\/j.cub.2025.10.041","article-title":"Light sensing in fungi","volume":"35","author":"Corrochano","year":"2025","journal-title":"Curr. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e00142-13","DOI":"10.1128\/mBio.00142-13","article-title":"The fungal pathogen Aspergillus fumigatus regulates growth, metabolism, and stress resistance in response to light","volume":"4","author":"Fuller","year":"2013","journal-title":"mBio"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1111\/jam.12381","article-title":"Effects of light on secondary metabolism and fungal development of Fusarium graminearum","volume":"116","author":"Kim","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Yu, Z., Streng, C., Seibeld, R.F., Igbalajobi, O.A., Leister, K., Ingelfinger, J., and Fischer, R. (2021). Genome-wide analyses of light-regulated genes in Aspergillus nidulans reveal a complex interplay between different photoreceptors and novel photoreceptor functions. PLoS Genet., 17.","DOI":"10.1371\/journal.pgen.1009845"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.1099\/mic.0.27346-0","article-title":"BLR-1 and BLR-2, key regulatory elements of photoconidiation and mycelial growth in Trichoderma atroviride","volume":"150","author":"Bibbins","year":"2004","journal-title":"Microbiology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.fgb.2010.02.001","article-title":"Crucial factors of the light perception machinery and their impact on growth and cellulase gene transcription in Trichoderma reesei","volume":"47","author":"Castellanos","year":"2010","journal-title":"Fungal Genet. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Yao, X., Guo, H., Zhang, K., Zhao, M., Ruan, J., and Chen, J. (2023). Trichoderma and its role in biological control of plant fungal and nematode disease. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1160551"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Allaga, H., Zhumakayev, A., B\u00fcchner, R., Kocsube, S., Sz\u0171cs, A., V\u00e1gv\u00f6lgyi, C., Kredics, L., and Hatvani, L. (2021). Members of the Trichoderma harzianum species complex with mushroom pathogenic potential. Agronomy, 11.","DOI":"10.3390\/agronomy11122434"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.3390\/microbiolres14030088","article-title":"Trichoderma species problematic to the commercial production of pleurotus in Italy: Characterization, identification, and methods of control","volume":"14","author":"Lombardi","year":"2023","journal-title":"Microbiol. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"\u0160a\u0161i\u0107 Zori\u0107, L., Janju\u0161evi\u0107, L., Djisalov, M., Kne\u017ei\u0107, T., Vunduk, J., Milenkovi\u0107, I., and Gadjanski, I. (2023). Molecular approaches for detection of Trichoderma green mold disease in edible mushroom production. Biology, 12.","DOI":"10.3390\/biology12020299"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1080\/15572536.2003.11833257","article-title":"Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus","volume":"94","author":"Samuels","year":"2002","journal-title":"Mycologia"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"104119","DOI":"10.1016\/j.tifs.2023.104119","article-title":"Effect of light on quality of preharvest and postharvest edible mushrooms and its action mechanism: A review","volume":"139","author":"Feng","year":"2023","journal-title":"Trends Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"De Bonis, M., Locatelli, S., Sambo, P., Zanin, G., Pecchia, J.A., and Nicoletto, C. (2024). Effect of different LED light wavelengths on production and quality of Pleurotus ostreatus grown on different commercial substrates. Horticulturae, 10.","DOI":"10.3390\/horticulturae10040349"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Moreno-Ruiz, D., Fuchs, A., Missbach, K., Schuhmacher, R., and Zeilinger, S. (2020). Influence of different light regimes on the mycoparasitic activity and 6-pentyl-\u03b1-pyrone biosynthesis in two strains of Trichoderma atroviride. Pathogens, 9.","DOI":"10.3390\/pathogens9100860"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Esquivel-Naranjo, E.U., Mancilla-Diaz, H., Marquez-Mazlin, R., Alizadeh, H., Kandula, D., Hampton, J., and Mendoza-Mendoza, A. (2024). Light regulates secreted metabolite production and antagonistic activity in Trichoderma. J. Fungi, 11.","DOI":"10.3390\/jof11010009"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.fgb.2010.04.010","article-title":"Trichoderma in the light of day\u2013physiology and development","volume":"47","author":"Schmoll","year":"2010","journal-title":"Fungal Genet. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.fgb.2010.12.009","article-title":"New insights into the mechanism of light modulated signaling by heterotrimeric G-proteins: ENVOY acts on gna1 and gna3 and adjusts cAMP levels in Trichoderma reesei (Hypocrea jecorina)","volume":"48","author":"Tisch","year":"2011","journal-title":"Fungal Genet. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6241","DOI":"10.1111\/1462-2920.15748","article-title":"Comprehensive analysis of the regulatory network of blue-light-regulated conidiation and hydrophobin production in Trichoderma guizhouense","volume":"23","author":"Li","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1002\/mlf2.12089","article-title":"The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense","volume":"2","author":"Li","year":"2023","journal-title":"mLife"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/s41579-022-00796-9","article-title":"Architecture of the dynamic fungal cell wall","volume":"21","author":"Gow","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e01145-22","DOI":"10.1128\/mbio.01145-22","article-title":"Modern biophysics redefines our understanding of fungal cell wall structure, complexity, and dynamics","volume":"13","author":"Wang","year":"2022","journal-title":"mBio"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.tim.2025.05.001","article-title":"Fungal cell walls: The rising importance of carbohydrate-active enzymes","volume":"33","author":"Yao","year":"2025","journal-title":"Trends Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s00253-025-13512-3","article-title":"Cell walls of filamentous fungi\u2013challenges and opportunities for biotechnology","volume":"109","author":"Ost","year":"2025","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"D571","DOI":"10.1093\/nar\/gkab1045","article-title":"The carbohydrate-active enzyme database: Functions and literature","volume":"50","author":"Drula","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"230","DOI":"10.2174\/1389202911314040002","article-title":"Regulation of cellulase and hemicellulase gene expression in fungi","volume":"14","author":"Amore","year":"2013","journal-title":"Curr. Genom."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Huang, X., Zhang, R., Qiu, Y., Wu, H., Xiang, Q., Yu, X., Zhao, K., Zhang, X., Chen, Q., and Penttinen, P. (2020). RNA-seq profiling showed divergent carbohydrate-active enzymes (CAZymes) expression patterns in Lentinula edodes at brown film formation stage under blue light induction. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01044"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1038\/s41598-023-28938-w","article-title":"MAPkinases regulate secondary metabolism, sexual development and light dependent cellulase regulation in Trichoderma reesei","volume":"13","author":"Schalamun","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1093\/oxfordjournals.molbev.a026092","article-title":"Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerse II subunit","volume":"16","author":"Liu","year":"1999","journal-title":"Mol. Bio. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: Improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Bio. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"msae263","DOI":"10.1093\/molbev\/msae263","article-title":"Molecular Evolutionary Genetics Analysis version 12 for adaptive and green computing","volume":"41","author":"Kumar","year":"2024","journal-title":"Mol. Bio. Evol."},{"key":"ref_34","first-page":"269","article-title":"Estimation of evolutionary distance between nucleotide sequences","volume":"1","author":"Tajima","year":"1984","journal-title":"Mol. Bio. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","article-title":"Confidence limits on phylogenies: An approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_36","first-page":"100291","article-title":"Effect of light spectra on cereal fungal pathogens, a review","volume":"43","author":"Balducci","year":"2022","journal-title":"Fungal Bio. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e2500966122","DOI":"10.1073\/pnas.2500966122","article-title":"Light controls gene functions through alternative splicing in fungi","volume":"122","author":"Li","year":"2025","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1099\/mic.0.052688-0","article-title":"Trichoderma: Sensing the environment for survival and dispersal","volume":"158","year":"2012","journal-title":"Microbiology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e71358","DOI":"10.7554\/eLife.71358","article-title":"Circadian oscillations in Trichoderma atroviride and the role of core clock components in secondary metabolism, development, and mycoparasitism against the phytopathogen Botrytis cinerea","volume":"11","author":"Spanner","year":"2022","journal-title":"eLife"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1080\/00275514.2000.12061150","article-title":"Interactions between the pathogen Trichoderma harzianum Th2 and Agaricus bisporus in mushroom compost","volume":"92","author":"Mamoun","year":"2000","journal-title":"Mycologia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s12600-024-01168-0","article-title":"Efficacy of disinfectants and heat treatments against green mould in casing soil and button mushroom (Agaricus bisporus) yield","volume":"52","year":"2024","journal-title":"Phytoparasitica"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Pola-S\u00e1nchez, E., Villalobos-Escobedo, J.M., Carreras-Villase\u00f1or, N., Mart\u00ednez-Hern\u00e1ndez, P., Beltr\u00e1n-Hern\u00e1ndez, E.B., Esquivel-Naranjo, E.U., and Herrera-Estrella, A. (2021). A global analysis of photoreceptor-mediated transcriptional changes reveals the intricate relationship between central metabolism and DNA repair in the filamentous fungus Trichoderma atroviride. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.724676"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Zhang, J., Wang, F., Liu, M., Fu, M., and Dong, C. (2022). Dynamic genome-wide transcription profiling and direct target genes of CmWC-1 reveal hierarchical light signal transduction in Cordyceps militaris. J. Fungi, 8.","DOI":"10.3390\/jof8060624"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhu, Y., Jia, C., Wang, C., Zhang, S., Yuan, X., Yang, J., Hsiang, T., Qi, B., Peng, Z., and Li, Y. (2024). Yield increment and transcriptome response caused by blue light treatment in Hericium coralloides. BMC Genom., 25.","DOI":"10.1186\/s12864-024-11108-1"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Song, L., Shrivastava, N., Gai, Y., Li, D., Cai, W., Shen, Y., Lin, F.C., Liu, J., and Wang, H. (2023). Role of the blue light receptor gene Icwc-1 in mycelium growth and fruiting body formation of Isaria cicadae. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1038034"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1002\/bies.20441","article-title":"The structure and synthesis of the fungal cell wall","volume":"28","author":"Bowman","year":"2006","journal-title":"BioEssays"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Garcia-Rubio, R., de Oliveira, H.C., Rivera, J., and Trevijano-Contador, N. (2020). The fungal cell wall: Candida, Cryptococcus, and Aspergillus species. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02993"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"RP100569","DOI":"10.7554\/eLife.100569.3","article-title":"\u03b2-1, 6-Glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall","volume":"13","author":"Bekirian","year":"2024","journal-title":"eLife"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Patel, P.K., and Free, S.J. (2019). The genetics and biochemistry of cell wall structure and synthesis in Neurospora crassa, a model filamentous fungus. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02294"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100140","DOI":"10.1016\/j.tcsw.2024.100140","article-title":"Characterization of the Neurospora crassa GH72 family of Laminarin\/Lichenin transferases and their roles in cell wall biogenesis","volume":"13","author":"Chatrath","year":"2025","journal-title":"Cell Surf."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1111\/cmi.12615","article-title":"Strengthening the fungal cell wall through chitin\u2013glucan cross-links: Effects on morphogenesis and cell integrity","volume":"18","author":"Arroyo","year":"2016","journal-title":"Cell. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1039\/D4CB00024B","article-title":"Carbohydrate-active enzyme (CAZyme) discovery and engineering via (Ultra) high-throughput screening","volume":"5","author":"Wardman","year":"2024","journal-title":"RSC Chem. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"102457","DOI":"10.1016\/j.cbpa.2024.102457","article-title":"Advances in the understanding and exploitation of carbohydrate-active enzymes","volume":"80","author":"Bains","year":"2024","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ygeno.2017.09.012","article-title":"Comparative transcriptomics of Pleurotus eryngii reveals blue-light regulation of carbohydrate-active enzymes (CAZymes) expression at primordium differentiated into fruiting body stage","volume":"110","author":"Xie","year":"2018","journal-title":"Genomics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13568-020-0951-x","article-title":"Transcriptomic profiling sheds light on the blue-light and red-light response of oyster mushroom (Pleurotus ostreatus)","volume":"10","author":"Wang","year":"2020","journal-title":"AMB Express"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Suriani Ribeiro, M., Graciano de Paula, R., Raquel Voltan, A., de Castro, R.G., Carraro, C.B., Jos\u00e9 de Assis, L., Stecca Steindorf, A., Henrique Goldman, G., Nascimento Silva, R., and Jos\u00e9 Ulhoa, C. (2019). Endo-\u03b2-1, 3-glucanase (GH16 family) from Trichoderma harzianum participates in cell wall biogenesis but is not essential for antagonism against plant pathogens. Biomolecules, 9.","DOI":"10.3390\/biom9120781"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/mmi.13256","article-title":"The N-acetylglucosamine catabolic gene cluster in Trichoderma reesei is controlled by the Ndt80-like transcription factor RON1","volume":"99","author":"Kappel","year":"2016","journal-title":"Mol. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.ijbiomac.2022.03.037","article-title":"The transcription factor Ron1 is required for chitin metabolism, asexual development and pathogenicity in Beauveria bassiana, an entomopathogenic fungus","volume":"206","author":"Qiu","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Virgilio, S., Cupertino, F.B., Ambrosio, D.L., and Bertolini, M.C. (2017). Regulation of the reserve carbohydrate metabolism by alkaline pH and calcium in Neurospora crassa reveals a possible cross-regulation of both signaling pathways. BMC Genom., 18.","DOI":"10.1186\/s12864-017-3832-1"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.funbio.2023.01.006","article-title":"A secondary function of trehalose-6-phosphate synthase is required for resistance to oxidative and desiccation stress in Fusarium verticillioides","volume":"127","author":"McMillan","year":"2023","journal-title":"Fungal Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"654251","DOI":"10.1155\/2012\/654251","article-title":"Protein glycosylation in Aspergillus fumigatus is essential for cell wall synthesis and serves as a promising model of multicellular eukaryotic development","volume":"2012","author":"Jin","year":"2012","journal-title":"Int. J. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2477","DOI":"10.1007\/s00253-018-8784-0","article-title":"Multiplicity of enzymatic functions in the CAZy AA3 family","volume":"102","author":"Laurent","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3909","DOI":"10.1099\/mic.0.2007\/007302-0","article-title":"Enhanced responsiveness and sensitivity to blue light by blr-2 overexpression in Trichoderma atroviride","volume":"153","year":"2007","journal-title":"Microbiology"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s40643-021-00461-8","article-title":"From induction to secretion: A complicated route for cellulase production in Trichoderma reesei","volume":"8","author":"Yan","year":"2021","journal-title":"Bioresour. Bioprocess."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Schalamun, M., Molin, E.M., and Schmoll, M. (2023). RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei. BMC genom., 24.","DOI":"10.1186\/s12864-023-09467-2"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Tisch, D., and Schmoll, M. (2013). Targets of light signalling in Trichoderma reesei. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-657"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/16\/1390\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:37:52Z","timestamp":1786696672000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/16\/1390"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":66,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["biology15161390"],"URL":"https:\/\/doi.org\/10.3390\/biology15161390","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}