{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T05:19:44Z","timestamp":1787721584020,"version":"build-2784847793"},"reference-count":165,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005074","name":"Kuwait Institute for Scientific Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005074","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/041tgg678","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Citrullus colocynthis (L.) Schrad., a drought-tolerant member of the Cucurbitaceae family, has been used in traditional medicine for centuries and has recently attracted increasing interest because of its potential pharmacological, agricultural, and ecological applications. This review examines current information on the plant\u2019s distribution, ethnomedicinal uses, phytochemical composition, biological activities, applications in poultry, and ecological significance. Its bioactive constituents include cucurbitacins, flavonoids, phenolic compounds, alkaloids, glycosides, fatty acids, and tocopherols. These compounds have been associated with anticancer, antidiabetic, antimicrobial, antioxidant, anti-inflammatory, neuroprotective, hepatoprotective, and other biological activities, although most of the available evidence comes from in-vitro and preclinical studies. Initial findings also indicate that the plant may have value as a phytogenic feed additive in poultry and as a drought-tolerant species for soil stabilization, rangeland rehabilitation, and desertification control. However, the practical application of these findings is still constrained by differences in research methods, variation in phytochemical composition, limited clinical evidence, and the plant\u2019s narrow therapeutic window and dose-dependent toxicity. Consequently, its therapeutic effectiveness and suitability for routine agricultural use cannot yet be established with certainty. Future research should focus on phytochemical standardization, quality control, thorough toxicological assessment, mechanistic studies, and well-designed clinical and animal trials using standardized preparations. Collectively, the available evidence indicates that C. colocynthis is a promising source of bioactive compounds with potential pharmaceutical, veterinary, and ecological applications, although rigorous scientific validation is required before its safe and effective utilization can be recommended.<\/jats:p>","DOI":"10.3390\/biology15171448","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:20:02Z","timestamp":1787563202000},"page":"1448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Neglected Desert Vine to Promising Bio-Resource: Phytochemistry, Biological Activities, Poultry Applications, and Ecological Significance of Citrullus colocynthis (L.) Schrad."],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1696-562X","authenticated-orcid":false,"given":"Hanan","family":"Al-Khalaifah","sequence":"first","affiliation":[{"name":"Food Security Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Kuwait City 13109, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahani","family":"Al-Surrayai","sequence":"additional","affiliation":[{"name":"Food Security Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Kuwait City 13109, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0865-1993","authenticated-orcid":false,"given":"Sheikh","family":"Shreaz","sequence":"additional","affiliation":[{"name":"Food Security Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Kuwait City 13109, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Alzamel","sequence":"additional","affiliation":[{"name":"Food Security Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box 24885, Kuwait City 13109, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Li, Q.-Y., Munawar, M., Saeed, M., Shen, J.-Q., Khan, M.S., Noreen, S., Alagawany, M., Naveed, M., Madni, A., and Li, C.-X. (2022). Citrullus colocynthis (L.) Schrad (Bitter Apple Fruit): Promising Traditional Uses, Pharmacological Effects, Aspects, and Potential Applications. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.791049"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Procter, M., Kundu, B., Sudalaimuthuasari, N., AlMaskari, R.S., Saeed, E.E., Hazzouri, K.M., and Amiri, K.M.A. (2022). Microbiome of Citrullus colocynthis (L.) Schrad. Reveals a Potential Association with Non-Photosynthetic Cyanobacteria. 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