{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T12:18:28Z","timestamp":1787746708583,"version":"build-2784847793"},"reference-count":35,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Fund for Stable Support to Agricultural Sci-Tech Renovation","award":["xjnkywdzc-2026001-13-08"],"award-info":[{"award-number":["xjnkywdzc-2026001-13-08"]}]},{"name":"Project of Fund for Stable Support to Agricultural Sci-Tech Renovation","award":["xjnkywdzc-2026001-15"],"award-info":[{"award-number":["xjnkywdzc-2026001-15"]}]},{"award":["xjnkywdzc-2026001-13-08"],"award-info":[{"award-number":["xjnkywdzc-2026001-13-08"]}],"id":[{"id":"https:\/\/ror.org\/023cbka75","id-type":"ROR","asserted-by":"publisher"}]},{"award":["xjnkywdzc-2026001-15"],"award-info":[{"award-number":["xjnkywdzc-2026001-15"]}],"id":[{"id":"https:\/\/ror.org\/023cbka75","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Xinjiang Uygur Autonomous Region Tianchi Talent-Young Doctor for Yahui Deng"},{"name":"project of Xinjiang Research Institute of Agriculture in Arid Areas","award":["Hqny-2025002"],"award-info":[{"award-number":["Hqny-2025002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>G. barbadense represents an indispensable germplasm resource for high-quality textile fiber and disease resistance; nevertheless, systematic information regarding its ZRT\/IRT-like protein (ZIP) gene family remains limited. Here, a total of 46 GbZIP genes were identified across the G. barbadense genome. Comprehensive bioinformatic investigations revealed uneven chromosomal distribution and confirmed that segmental\/whole-genome duplications, supplemented by localized tandem duplications, drove family expansion. Members clustered within the same phylogenetic clades shared conserved motif organization and gene architecture, while promoter regions harbored abundant cis-acting elements associated with phytohormone and stress signaling. Transcriptome profiling indicated distinct expression patterns across vegetative\/reproductive tissues, fiber and ovule developmental stages, and diverse abiotic stress conditions (cold, hot, drought, and salt). Quantitative Real-Time PCR (qRT-PCR) further validated that several GbZIP candidates exhibited temporal expression variations upon exposure to cadmium toxicity, V. dahliae infection, and combined Cd-V. dahliae stress. Specifically, GbZIP13, GbZIP18, GbZIP27, and GbZIP36 displayed prominent broad-spectrum responses to all three stress conditions, whereas GbZIP16, GbZIP29, and GbZIP30 showed stress-specific regulatory divergence. Overall, this study aims to systematically analyze the evolutionary characteristics and expression patterns of the GbZIP family, and to specifically evaluate the response differences under Cd stress, V. dahliae stress, and combined stress, in order to identify potential key candidate genes.<\/jats:p>","DOI":"10.3390\/biology15171455","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:59:23Z","timestamp":1787741963000},"page":"1455","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Characterization of the ZIP Transporter Family in Sea Island Cotton (Gossypium barbadense L.) and Expression Profiling Under Heavy Metal and Pathogen Stresses"],"prefix":"10.3390","volume":"15","author":[{"given":"Yahui","family":"Deng","sequence":"first","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5218-1048","authenticated-orcid":false,"given":"Nan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jidi","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caixia","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingfang","family":"Ran","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaohua","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alifu","family":"Aierxi","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wumaierjiang","family":"Kuerban","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Kong","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0868-700X","authenticated-orcid":false,"given":"Weiran","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production, National Cotton Engineering Technology Research Center, Cotton Research Institute of Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jare.2023.02.002","article-title":"Genome and haplotype provide insights into the population differentiation and 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