{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:18:13Z","timestamp":1787890693287,"version":"build-2784847793"},"reference-count":56,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fujian Provincial Forestry Science and Technology Project","award":["ZMGG-0809"],"award-info":[{"award-number":["ZMGG-0809"]}]},{"name":"2026 Science and Technology Innovation Special Fund of Fujian Province","award":["KFB23047"],"award-info":[{"award-number":["KFB23047"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Phoebe bournei is an economically and ecologically important woody species native to China. As a core component of colchicine-triggered polyploid breeding, the tubulin genes (TUA and TUB) have been identified and functionally analyzed in many plants, but not yet in P. bournei. Here, tubulin family members in P. bournei were identified through sequence alignment and subsequently characterized using comprehensive bioinformatic analyses. In particular, a total of six PbTUA and ten PbTUB members were identified and grouped into two and five subfamilies, respectively, according to phylogenetic relationships. Most tubulin proteins were small (414\u2013522 aa) with predicted stability. Furthermore, 36 collinear gene pairs were identified, suggesting a possible contribution to the evolutionary expansion of this family. For different tissues, the expression levels of most tubulin genes were generally lower in leaves but higher in roots. Besides, treatment with 1.0% colchicine inhibited the expression of all 15 tubulin genes except PbTUB6. These results provide preliminary insights into tubulin genes associated with polyploid induction and supply candidate genes for future functional studies toward polyploid germplasm creation of P. bournei.<\/jats:p>","DOI":"10.3390\/biology15171456","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:04:32Z","timestamp":1787756672000},"page":"1456","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Identification and Colchicine-Responsive Expression Profiling of the Tubulins (TUA and TUB) Gene Family in Phoebe bournei"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9622-2171","authenticated-orcid":false,"given":"Xiaowen","family":"Li","sequence":"first","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Shen","sequence":"additional","affiliation":[{"name":"Fujian Province Yunxiao County Forestry Bureau, Zhangzhou 363300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeping","family":"Jian","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxin","family":"Mei","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shipin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8129-2081","authenticated-orcid":false,"given":"Haichao","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.foreco.2012.06.044","article-title":"Evaluation of the effects of forest management strategies on carbon sequestration in evergreen broad-leaved (Phoebe bournei) plantation forests using FORECAST ecosystem model","volume":"300","author":"Wang","year":"2013","journal-title":"For. Ecol. Manag."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100410","DOI":"10.1016\/j.xplc.2022.100410","article-title":"The chromosome-scale genome of Phoebe bournei reveals contrasting fates of terpene synthase (TPS)-a and TPS-b subfamilies","volume":"3","author":"Han","year":"2022","journal-title":"Plant Commun."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wang, D., Li, J., and Tang, T. (2022). Determining the Optimal Density of Phoebe bournei Plantations Based on Dynamic Programming under Close-to-Nature Management Measures. Sustainability, 14.","DOI":"10.3390\/su14020847"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Li, X., Liu, L., Sun, S., Li, Y., Jia, L., Ye, S., Yu, Y., Dossa, K., and Luan, Y. (2022). Leaf-transcriptome profiles of phoebe bournei provide insights into temporal drought stress responses. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1010314"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4057","DOI":"10.1016\/j.cub.2022.07.019","article-title":"Polyploidy increases storage but decreases structural stability in Arabidopsis thaliana","volume":"32","author":"Pacey","year":"2022","journal-title":"Curr. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"123050","DOI":"10.1016\/j.indcrop.2026.123050","article-title":"A novel and tissue-culture-free polyploidization method based on adventitious bud formation of Populus","volume":"242","author":"Wang","year":"2026","journal-title":"Ind. Crops Prod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s11240-024-02953-9","article-title":"Generation and characterization of a tetraploid Populus davidiana","volume":"160","author":"Wang","year":"2025","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"121976","DOI":"10.1016\/j.indcrop.2025.121976","article-title":"Efficient creation and phenotypic differentiation mechanism of autotetraploid soybean","volume":"236","author":"Zhai","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"113031","DOI":"10.1016\/j.scienta.2024.113031","article-title":"Elevating morphology and essential oil in cumin genotypes through polyploidy induction","volume":"329","author":"Mortazavian","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_10","first-page":"4","article-title":"Breeding polyploid Populus: Progress and perspective","volume":"2","author":"Kang","year":"2022","journal-title":"For. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"121338","DOI":"10.1016\/j.indcrop.2025.121338","article-title":"Colchicine-induced chromosome doubling in Cinnamomum camphora and its effect on morphological and terpenoid storage structures","volume":"233","author":"Chen","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s13007-025-01426-0","article-title":"Efficient induction of tetraploids via adventitious bud regeneration and subsequent phenotypic variation in Acacia melanoxylon","volume":"21","author":"Jiang","year":"2025","journal-title":"Plant Methods"},{"key":"ref_13","first-page":"119","article-title":"Pollen chromosome doubling induced by colchicine treatment and creation of hybrid triploids in Populus canescens","volume":"42","author":"Zhou","year":"2020","journal-title":"J. Beijing For. Univ."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bian, G., Yi, Y., Song, Z., Huang, Y., Mao, Q., Qin, J., and Shang, X. (2025). Colchicine-Induced Polyploidization Influences the Morphological, Physiological, and Biochemical Characteristics of Cyclocarya paliurus. Plants, 14.","DOI":"10.3390\/plants14172778"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zhang, R., Rao, S., Wang, Y., Qin, Y., Qin, K., and Chen, J. (2024). Chromosome Doubling Enhances Biomass and Carotenoid Content in Lycium chinense. Plants, 13.","DOI":"10.3390\/plants13030439"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wang, L., Zheng, P., Ge, H., Zhao, X., Kou, Y., Yang, S., Yu, X., and Jia, R. (2024). Colchicine-Induced Tetraploidy in Protocorms of Aerides rosea Lodd. ex Lindl. and Paxton. and Its Identification. Plants, 13.","DOI":"10.3390\/plants13243535"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bharati, R., and Severov\u00e1, L. (2025). Artificial polyploidy as a tool for improving growth and stress resilience in tree species. Front. For. Glob. Change, 8.","DOI":"10.3389\/ffgc.2025.1569384"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s11240-010-9786-5","article-title":"Mitotic chromosome doubling of plant tissues in vitro","volume":"104","author":"Dhooghe","year":"2011","journal-title":"Plant Cell Tissue Organ Cult. (PCTOC)"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00709-011-0339-5","article-title":"Microtubules and the tax payer","volume":"249","author":"Nick","year":"2012","journal-title":"Protoplasma"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"19258","DOI":"10.1038\/srep19258","article-title":"Characterization and putative post-translational regulation of \u03b1- and \u03b2-tubulin gene families in Salix arbutifolia","volume":"6","author":"Rao","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Mao, J., Jia, C., Ling, J., and Chen, Y. (2025). Genome-wide identification, characterization and expression analysis of tubulin gene family in Populus deltoides. BMC Plant Biol., 25.","DOI":"10.1186\/s12870-025-06228-z"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Pais, E., and Schou, K.B. (2026). Microtubule-Associated Proteins: From Dynamic Regulation of Microtubules to Cellular Architecture. Cells, 15.","DOI":"10.3390\/cells15141289"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Yang, K., Li, H., Jiang, N., Wang, L., Liu, H., Yang, Y., and Wuyun, T. (2026). Evolutionary Dynamics of the Tubulin Gene Family Across Plants and Identification of PaTUA1 as a Candidate Gene Associated with Apricot Kernel Development. Horticulturae, 12.","DOI":"10.3390\/horticulturae12070837"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"eads3653","DOI":"10.1126\/sciadv.ads3653","article-title":"Phase separation of the poplar microtubule-associated protein PagPCaP1a aids microtubule depolymerization in response to high salt","volume":"11","author":"Lian","year":"2025","journal-title":"Sci. Adv."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3747","DOI":"10.1016\/j.cub.2024.07.020","article-title":"An Arabidopsis Kinesin-14D motor is associated with midzone microtubules for spindle morphogenesis","volume":"34","author":"Guo","year":"2024","journal-title":"Curr. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1016\/j.devcel.2024.08.001","article-title":"The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway","volume":"59","author":"Saville","year":"2024","journal-title":"Dev. Cell"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Maekawa, K., van Gessel, N., Reski, R., and Kozgunova, E. (2025). CRISPR\/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1653765"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e2316583121","DOI":"10.1073\/pnas.2316583121","article-title":"A coadapted KNL1 and spindle assembly checkpoint axis orchestrates precise mitosis in Arabidopsis","volume":"121","author":"Deng","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"kiaf461","DOI":"10.1093\/plphys\/kiaf461","article-title":"Functional framework of the kinetochore and spindle assembly checkpoint in Arabidopsis","volume":"199","author":"Magyar","year":"2025","journal-title":"Plant Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.devcel.2017.09.017","article-title":"The Spindle Assembly Checkpoint in Arabidopsis Is Rapidly Shut Off during Severe Stress","volume":"43","author":"Komaki","year":"2017","journal-title":"Dev. Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"eadq4033","DOI":"10.1126\/sciadv.adq4033","article-title":"Arabidopsis KNL1 recruits type one protein phosphatase to kinetochores to silence the spindle assembly checkpoint","volume":"11","author":"He","year":"2025","journal-title":"Sci. Adv."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3103\/S0095452719010067","article-title":"Analysis of \u03b1-Tubulin Gene Expression During Cold Acclimation of Winter and Spring Soft Wheat","volume":"53","author":"Buy","year":"2019","journal-title":"Cytol. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Gavazzi, F., Pigna, G., Braglia, L., Gian\u00ec, S., Breviario, D., and Morello, L. (2017). Evolutionary characterization and transcript profiling of \u03b2-tubulin genes in flax (Linum usitatissimum L.) during plant development. BMC Plant Biol., 17.","DOI":"10.1186\/s12870-017-1186-0"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s42397-021-00097-1","article-title":"Identification and expression analysis of Tubulin gene family in upland cotton","volume":"4","author":"Chen","year":"2021","journal-title":"J. Cotton Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","article-title":"TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data","volume":"13","author":"Chen","year":"2020","journal-title":"Mol. Plant"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"msae263","DOI":"10.1093\/molbev\/msae263","article-title":"MEGA12: Molecular Evolutionary Genetic Analysis Version 12 for Adaptive and Green Computing","volume":"41","author":"Kumar","year":"2024","journal-title":"Mol. Biol. Evol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Li, S., Cao, P., Wang, C., Guo, J., Zang, Y., Wu, K., Ran, F., Liu, L., Wang, D., and Min, Y. (2021). Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family Member FtsZ2-1 during Various Stress. Plants, 10.","DOI":"10.3390\/plants10040668"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/cm.21099","article-title":"Dissecting the cellular functions of plant microtubules using mutant tubulins","volume":"70","author":"Hashimoto","year":"2013","journal-title":"Cytoskeleton"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1146\/annurev.arplant.043008.092122","article-title":"Bias in plant gene content following different sorts of duplication: Tandem, whole-genome, segmental, or by transposition","volume":"60","author":"Freeling","year":"2009","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Singh, B., Yun, S., Gil, Y., and Park, M.-H. (2025). The Role of Colchicine in Plant Breeding. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26146743"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"173","DOI":"10.2174\/1874070702115010173","article-title":"Colchicine Induced Mutation in Nigella sativa Plant for the Assessment of Morpho-Physiological and Biochemical Parameter Vis-A-Vis In Vitro Anti-Inflammatory Activity","volume":"15","author":"Gupta","year":"2021","journal-title":"Open Biotechnol. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.13057\/biodiv\/d240310","article-title":"Effect of colchicine mutagen on phenotype and genotype of Vigna unguiculata var. sesquipedalis the 7th generation","volume":"24","author":"Fathurrahman","year":"2023","journal-title":"Biodiversitas"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1016\/j.cj.2024.09.011","article-title":"Vitamin and zeatin treatments promote colchicine-induced haploid chromosome doubling in maize","volume":"12","author":"Chen","year":"2024","journal-title":"Crop J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9963","DOI":"10.1038\/s41467-024-54036-0","article-title":"Soluble \u03b1\u03b2-tubulins reversibly sequester TTC5 to regulate tubulin mRNA decay","volume":"15","author":"Batiuk","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/nrg2689","article-title":"The evolution of gene duplications: Classifying and distinguishing between models","volume":"11","author":"Innan","year":"2010","journal-title":"Nat. Rev. Genet."},{"key":"ref_46","first-page":"539","article-title":"The small genome of Arabidopsis contains at least six expressed alpha-tubulin genes","volume":"4","author":"Kopczak","year":"1992","journal-title":"Plant Cell"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0022-2836(92)90683-B","article-title":"Alpha-tubulin gene family of maize (Zea mays L.). Evidence for two ancient alpha-tubulin genes in plants","volume":"227","author":"Villemur","year":"1992","journal-title":"J. Mol. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Ren, Y., Song, Q., Shan, S., Wang, J., Ma, S., Song, Y., Ma, L., Zhang, G., and Niu, N. (2022). Genome-Wide Identification and Expression Analysis of TUA and TUB Genes in Wheat (Triticum aestivum L.) during Its Development. Plants, 11.","DOI":"10.3390\/plants11243495"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1104\/pp.107.107086","article-title":"Differential expansion and expression of alpha- and beta-tubulin gene families in Populus","volume":"145","author":"Oakley","year":"2007","journal-title":"Plant Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2294","DOI":"10.1104\/pp.16.00523","article-title":"Evolution of Gene Duplication in Plants","volume":"171","author":"Panchy","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Nielsen, M.G., Gadagkar, S.R., and Gutzwiller, L. (2010). Tubulin evolution in insects: Gene duplication and subfunctionalization provide specialized isoforms in a functionally constrained gene family. BMC Evol. Biol., 10.","DOI":"10.1186\/1471-2148-10-113"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Girardini, K.N., Olthof, A.M., and Kanadia, R.N. (2023). Introns: The \u201cdark matter\u201d of the eukaryotic genome. Front. Genet., 14.","DOI":"10.3389\/fgene.2023.1150212"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1046\/j.0960-7412.2001.01192.x","article-title":"A long leader intron of the Ostub16 rice beta-tubulin gene is required for high-level gene expression and can autonomously promote transcription both in vivo and in vitro","volume":"29","author":"Morello","year":"2002","journal-title":"Plant J. Cell Mol. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"He, Y., Fan, L., Wang, R., Han, S., Sun, G., Yu, F., Yang, Q., Yang, H., and Zhang, G. (2024). Evolutionary Characterization of tubulin Gene Family in the Desert Biomass Willow (Salix psammophila) and Expression of the \u03b2-tubulin Gene SpsTUB10 during Different Stresses. Forests, 15.","DOI":"10.3390\/f15040696"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Blume, R.Y., Rabokon, A.M., Pydiura, M., Yemets, A.I., Pirko, Y.V., and Blume, Y.B. (2024). Genome-wide identification and evolution of the tubulin gene family in Camelina sativa. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10503-y"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1093\/plphys\/kiae329","article-title":"Tubulin participates in establishing protoxylem vessel reinforcement patterns and hydraulic conductivity in maize","volume":"196","author":"Huang","year":"2024","journal-title":"Plant Physiol."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/17\/1456\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:14:25Z","timestamp":1787890465000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/17\/1456"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,26]]},"references-count":56,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["biology15171456"],"URL":"https:\/\/doi.org\/10.3390\/biology15171456","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,26]]}}}