{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:15:09Z","timestamp":1788527709847,"version":"build-2803163510"},"reference-count":75,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Canavanine (CAN) is a nonproteinogenic amino acid, synthesised by many legumes (e.g., alfalfa, hairy vetch) and acts as a strong inhibitor of root growth. Thus, CAN may be considered as an allelopathic compound, influencing natural and agroecosystems. CAN is a structural analogue of arginine (Arg), so its toxicity results from its primary mode of action due to interference with Arg-dependent processes, especially incorporation into proteins instead of Arg. The aim of the work was to investigate the impact of CAN (10, 50 \u00b5M) treatment (24 or 72 h) on Arg catabolism and content of polyamines (PAs) in the roots of tomato (Solanum lycopersicum L.) seedlings. In the roots of plants cultured in the presence of CAN for 72 h, whosegrowth was completely inhibited, arginase activity and transcript levels of ARG1 and ARG2 were elevated, while ornithine (the product of Arg catabolism by arginase) content was low. CAN application resulted also in decreased total PAs level and increased relative contribution of spermine in the roots of tomato seedlings. CAN-induced alterations in PAs content in the roots were accompanied by changes in transcript levels of genes related to PAs synthesis (SPMS, SPDS) and PAs catabolism (PAO1).<\/jats:p>","DOI":"10.3390\/biology15171541","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:20:46Z","timestamp":1788524446000},"page":"1541","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alterations in Arginase Activity and Polyamine Metabolism Contribute to the Mode of Action of Canavanine in Tomato Roots"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8532-5151","authenticated-orcid":false,"given":"Pawel","family":"Staszek","sequence":"first","affiliation":[{"name":"Department of Botany and Plant Physiology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1485-2901","authenticated-orcid":false,"given":"Katarzyna","family":"Ciacka","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Physiology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0077-3743","authenticated-orcid":false,"given":"Agnieszka","family":"Gniazdowska","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Physiology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0305-1978(77)90007-2","article-title":"Nitrogen Allocation for L-Canavanine Synthesis and Its Relationship to Chemical Defense of the Seed","volume":"5","author":"Rosenthal","year":"1977","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1016\/S0031-9422(00)89853-2","article-title":"Canavanine in Canavalia paraguayensis, C. gladiata and Dioclea paraguayensis","volume":"11","author":"Bordas","year":"1972","journal-title":"Phytochemistry"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1007\/s11101-017-9536-y","article-title":"L-Canavanine: How Does a Simple Non-Protein Amino Acid Inhibit Cellular Function in a Diverse Living System?","volume":"16","author":"Staszek","year":"2017","journal-title":"Phytochem. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1076\/1388-0209(200001)3811-BFT001","article-title":"The Natural Abundance Of L-Canavanine, An Active Anticancer Agent, in Alfalfa, Medicago sativa (L.)","volume":"38","author":"Rosenthal","year":"2000","journal-title":"Pharm. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1007\/BF00981932","article-title":"Metabolism and Exudation of Canavanine during Development of Alfalfa (Medicago sativa L. Cv. Verko)","volume":"18","author":"Miersch","year":"1992","journal-title":"J. Chem. Ecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e23994","DOI":"10.4161\/psb.23994","article-title":"Inhibition of Tomato (Solanum lycopersicum L.) Root Growth by Cyanamide Is Not Always Accompanied with Enhancement of ROS Production","volume":"8","author":"Soltys","year":"2013","journal-title":"Plant Signal. Behav."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s00425-011-1429-5","article-title":"Cyanamide Mode of Action during Inhibition of Onion (Allium cepa L.) Root Growth Involves Disturbances in Cell Division and Cytoskeleton Formation","volume":"234","author":"Soltys","year":"2011","journal-title":"Planta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.jplph.2014.01.004","article-title":"Phytotoxic Cyanamide Affects Maize (Zea mays) Root Growth and Root Tip Function: From Structure to Gene Expression","volume":"171","author":"Soltys","year":"2014","journal-title":"J. Plant Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10527","DOI":"10.1038\/srep10527","article-title":"Cyanamide Is Biosynthesized from L-Canavanine in Plants","volume":"5","author":"Kamo","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1007\/s11627-019-09985-3","article-title":"Evaluation of Canavanine as an Allelochemical in Etiolated Seedlings of Vicia villosa Roth: Protoplast Co-Culture Method with Digital Image Analysis","volume":"55","author":"Sasamoto","year":"2019","journal-title":"In Vitro Cell. Dev. Biol.\u2014Plant"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mardani-Korrani, H., Nakayasu, M., Yamazaki, S., Aoki, Y., Kaida, R., Motobayashi, T., Kobayashi, M., Ohkama-Ohtsu, N., Oikawa, Y., and Sugiyama, A. (2021). L-Canavanine, a Root Exudate From Hairy Vetch (Vicia villosa) Drastically Affecting the Soil Microbial Community and Metabolite Pathways. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.701796"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ignacio, M., Rodr\u00edguez-Robles, D., Zambelli, A., Nocito, F.F., and Araniti, F. (2025). Unveiling the Multifaceted Roles of Root Exudates: Chemical Interactions, Allelopathy, and Agricultural Applications. Agronomy, 15.","DOI":"10.3390\/agronomy15040845"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1111\/j.1742-4658.2009.06866.x","article-title":"Amino Acid Discrimination by Arginyl-TRNA Synthetases as Revealed by an Examination of Natural Specificity Variants","volume":"276","author":"Igloi","year":"2009","journal-title":"FEBS J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s007260170017","article-title":"L-Canavanine: A Higher Plant Insecticidal Allelochemical","volume":"21","author":"Rosenthal","year":"2001","journal-title":"Amino Acids"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0300-9629(75)90409-0","article-title":"Non-Protein Amino Acid-Insect Interactions\u2014I. Growth Effects and Symptomology of l-Canavanine Consumption by Tobacco Hornworm, Manduca sexta (L.)","volume":"51","author":"Dahlman","year":"1975","journal-title":"Comp. Biochem. Physiol. A Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0022-1910(76)90035-4","article-title":"Further Studies of the Effect of L-Canavanine on the Tobacco Hornworm, Manduca sexta","volume":"22","author":"Dahlman","year":"1976","journal-title":"J. Insect Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Devambez, I., Ali Agha, M., Mitri, C., Bockaert, J., Parmentier, M.-L., Marion-Poll, F., Grau, Y., and Soustelle, L. (2013). G\u03b1o Is Required for L-Canavanine Detection in Drosophila. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0063484"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1097\/CAD.0b013e32833e0334","article-title":"V Canavanine Augments Proapoptotic Effects of Arginine Deprivation in Cultured Human Cancer Cells","volume":"22","author":"Vynnytska","year":"2011","journal-title":"Anticancer Drugs"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1023\/A:1022435202066","article-title":"L-Canavanine as a Radiosensitization Agent for Human Pancreatic Cancer Cells","volume":"244","author":"Bence","year":"2003","journal-title":"Mol. Cell. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e1542","DOI":"10.7717\/peerj.1542","article-title":"L-Canavanine Potentiates the Cytotoxicity of Doxorubicin and Cisplatin in Arginine Deprived Human Cancer Cells","volume":"4","author":"Nurcahyanti","year":"2016","journal-title":"PeerJ"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1104\/pp.108.117432","article-title":"Differential Regulation of Root Arginine Catabolism and Polyamine Metabolism in Clubroot-Susceptible and Partially Resistant Arabidopsis Genotypes","volume":"146","author":"Jubault","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Winter, G., Todd, C.D., Trovato, M., Forlani, G., and Funck, D. (2015). Physiological Implications of Arginine Metabolism in Plants. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00534"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s00425-020-03411-4","article-title":"Peroxynitrite Induced Signaling Pathways in Plant Response to Non-Proteinogenic Amino Acids","volume":"252","author":"Staszek","year":"2020","journal-title":"Planta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1104\/pp.108.121459","article-title":"Arginase-Negative Mutants of Arabidopsis Exhibit Increased Nitric Oxide Signaling in Root Development","volume":"147","author":"Flores","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"45998","DOI":"10.1074\/jbc.M407151200","article-title":"Regulation of Plant Arginase by Wounding, Jasmonate, and the Phytotoxin Coronatine","volume":"279","author":"Chen","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.plaphy.2016.03.005","article-title":"Toxicity of Canavanine in Tomato (Solanum lycopersicum L.) Roots Is Due to Alterations in RNS, ROS and Auxin Levels","volume":"103","author":"Krasuska","year":"2016","journal-title":"Plant Physiol. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Krasuska, U., Andrzejczak, O., Staszek, P., Bogatek, R., and Gniazdowska, A. (2016). Canavanine Alters ROS\/RNS Level and Leads to Post-Translational Modification of Proteins in Roots of Tomato Seedlings. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00840"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Staszek, P., Krasuska, U., Otulak-Kozie\u0142, K., Fettke, J., and Gniazdowska, A. (2019). Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.01077"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"113190","DOI":"10.1016\/j.plantsci.2026.113190","article-title":"Current Directions in Plant Nitric Oxide Research","volume":"369","author":"Kolbert","year":"2026","journal-title":"Plant Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s00425-016-2553-z","article-title":"Modification of the Endogenous NO Level Influences Apple Embryos Dormancy by Alterations of Nitrated and Biotinylated Protein Patterns","volume":"244","author":"Krasuska","year":"2016","journal-title":"Planta"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, W., Paschalidis, K., Feng, J.C., Song, J., and Liu, J.H. (2019). Polyamine Catabolism in Plants: A Universal Process with Diverse Functions. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00561"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Hern\u00e1ndez, A.I., Scalschi, L., Vicedo, B., Marcos-Barbero, E.L., Morcuende, R., and Cama\u00f1es, G. (2022). Putrescine: A Key Metabolite Involved in Plant Development, Tolerance and Resistance Responses to Stress. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23062971"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1104\/pp.118.1.323","article-title":"Expression of Ornithine Decarboxylase Is Transiently Increased by Pollination, 2,4-Dichlorophenoxyacetic Acid, and Gibberellic Acid in Tomato Ovaries","volume":"118","author":"Carbonell","year":"1998","journal-title":"Plant Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1046\/j.1365-313X.2001.01100.x","article-title":"Arabidopsis Polyamine Biosynthesis: Absence of Ornithine Decarboxylase and the Mechanism of Arginine Decarboxylase Activity","volume":"27","author":"Hanfrey","year":"2001","journal-title":"Plant J."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chen, D., Shao, Q., Yin, L., Younis, A., and Zheng, B. (2019). Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.01945"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Zhu, M., Chen, G., Wu, J., Wang, J., Wang, Y., Guo, S., and Shu, S. (2023). Identification of Cucumber S-Adenosylmethionine Decarboxylase Genes and Functional Analysis of CsSAMDC3 in Salt Tolerance. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1076153"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.plaphy.2021.05.018","article-title":"Overexpression of the White Clover TrSAMDC1 Gene Enhanced Salt and Drought Resistance in Arabidopsis thaliana","volume":"165","author":"Jia","year":"2021","journal-title":"Plant Physiol. Biochem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1046\/j.1365-313X.1996.09020147.x","article-title":"Potato Plants Expressing Antisense and Sense S-Adenosylmethionine Decarboxylase (SAMDC) Transgenes Show Altered Levels of Polyamines and Ethylene: Antisense Plants Display Abnormal Phenotypes","volume":"9","author":"Kumar","year":"1996","journal-title":"Plant J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"317","DOI":"10.4161\/psb.2.4.4116","article-title":"ROS and Phytohormones in Plant-Plant Allelopathic Interaction","volume":"2","author":"Bogatek","year":"2007","journal-title":"Plant Signal. Behav."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/978-3-319-10079-1_8","article-title":"Allelopathic Compounds as Oxidative Stress Agents: Yes or NO","volume":"Volume 23","author":"Gupta","year":"2015","journal-title":"Reactive Oxygen and Nitrogen Species Signaling and Communication in Plants, Signaling and Communication in Plants"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1007\/s00709-021-01729-8","article-title":"Plant Cell Responses to Allelopathy: From Oxidative Stress to Programmed Cell Death","volume":"259","author":"Koce","year":"2022","journal-title":"Protoplasma"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Staszek, P., Krasuska, U., Wal, A., Zak, J., and Gniazdowska, A. (2022). NO and Metabolic Reprogramming under Phytotoxicity Stress. Nitric Oxide in Plant Biology: An Ancient Molecule with Emerging Roles, Elsevier.","DOI":"10.1016\/B978-0-12-818797-5.00022-4"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1016\/j.sjbs.2021.02.052","article-title":"Exogenously Applied Spermidine Confers Protection against Cinnamic Acid-Mediated Oxidative Stress in Pisum sativum","volume":"28","author":"Kapoor","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Shao, J., Huang, K., Batool, M., Idrees, F., Afzal, R., Haroon, M., Noushahi, H.A., Wu, W., Hu, Q., and Lu, X. (2022). Versatile Roles of Polyamines in Improving Abiotic Stress Tolerance of Plants. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1003155"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Liu, J.H., Wang, W., Wu, H., Gong, X., and Moriguchi, T. (2015). Polyamines Function in Stress Tolerance: From Synthesis to Regulation. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00827"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1111\/ppa.12537","article-title":"Arginase Activity in Arabidopsis thaliana Infected with Heterodera schachtii","volume":"65","author":"Labudda","year":"2016","journal-title":"Plant Pathol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"A.3A.1","DOI":"10.1002\/0471141755.pha03as38","article-title":"Assays for Determination of Protein Concentration","volume":"38","author":"Olson","year":"2007","journal-title":"Curr. Protoc. Pharmacol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.niox.2016.11.003","article-title":"Nitric Oxide-Polyamines Cross-Talk during Dormancy Release and Germination of Apple Embryos","volume":"68","author":"Krasuska","year":"2017","journal-title":"Nitric Oxide"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/gkp045","article-title":"Amplification Efficiency: Linking Baseline and Bias in the Analysis of Quantitative PCR Data","volume":"37","author":"Ruijter","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"R19","DOI":"10.1186\/gb-2007-8-2-r19","article-title":"QBase Relative Quantification Framework and Software for Management and Automated Analysis of Real-Time Quantitative PCR Data","volume":"8","author":"Hellemans","year":"2007","journal-title":"Genome Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1104\/pp.107.4.1097","article-title":"Essential Role of Urease in Germination of Nitrogen-Limited Arabidopsis thaliana Seeds","volume":"107","author":"Zonia","year":"1995","journal-title":"Plant Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1006\/bbrc.1994.1029","article-title":"Comparison of Substrate and Inhibitor Specificity of Arginase and Nitricm Oxide (NO) Synthase for Arginine Analogs and Related Compounds in Murine and Rat Macrophages","volume":"198","author":"Bajor","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1042\/bj1530469","article-title":"The Heterogeneity of Arginases in Rat Tissues","volume":"153","author":"Herzfeld","year":"1976","journal-title":"Biochem. J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.plaphy.2020.10.002","article-title":"What We Know about Plant Arginases?","volume":"156","author":"Siddappa","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.1021\/bi002318+","article-title":"Mechanistic and Metabolic Inferences from the Binding of Substrate Analogues and Products to Arginase","volume":"40","author":"Cox","year":"2001","journal-title":"Biochemistry"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1042\/bj1750449","article-title":"Purification and Properties of Arginase from Human Liver and Erythrocytes","volume":"175","author":"Colombo","year":"1978","journal-title":"Biochem. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1161\/CIRCRESAHA.107.157727","article-title":"Inducible NO Synthase-Dependent S-Nitrosylation and Activation of Arginase1 Contribute to Age-Related Endothelial Dysfunction","volume":"101","author":"Santhanam","year":"2007","journal-title":"Circ. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3934","DOI":"10.1016\/j.bmcl.2005.05.088","article-title":"L-Arginine Analogs as Alternate Substrates for Nitric Oxide Synthase","volume":"15","author":"Luzzi","year":"2005","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1006\/bbrc.1994.1909","article-title":"Inhibition of Rat Liver Arginase by an Intermediate in NO Biosynthesis, NG-Hydroxy-L-Arginine: Implications for the Regulation of Nitric Oxide Biosynthesis by Arginase","volume":"202","author":"Daghigh","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1104\/pp.73.4.965","article-title":"L-Arginine and L-Canavanine Metabolism in Jack Bean, Canavalia Ensiformis (L.) DC. and Soybean, Glycine Max (L.) Merr","volume":"73","author":"Downum","year":"1983","journal-title":"Plant Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Majumdar, R., Barchi, B., Turlapati, S.A., Gagne, M., Minocha, R., Long, S., and Minocha, S.C. (2016). Glutamate, Ornithine, Arginine, Proline, and Polyamine Metabolic Interactions: The Pathway Is Regulated at the Post-Transcriptional Level. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00078"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1111\/j.1469-8137.2009.02823.x","article-title":"The Importance of Cytosolic Glutamine Synthetase in Nitrogen Assimilation and Recycling","volume":"182","author":"Bernard","year":"2009","journal-title":"New Phytol."},{"key":"ref_63","first-page":"365","article-title":"Involvement of Polyamines in Plant Growth and Senescence","volume":"3","author":"Altman","year":"1981","journal-title":"Adv. Polyam. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0176-1617(89)80001-X","article-title":"Daucus carota Cell Cultures: Polyamines and Effect of Polyamine Biosynthesis Inhibitors in the Preembryogenic Phase and Different Embryo Stages","volume":"134","author":"Mengoli","year":"1989","journal-title":"J. Plant Physiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/S0176-1617(97)80237-4","article-title":"Polyamines, Auxins and Organogenesis in Leafy Spurge (Euphorbia esula L.)","volume":"151","author":"Davis","year":"1997","journal-title":"J. Plant Physiol."},{"key":"ref_66","first-page":"565","article-title":"Polyamine Metabolism in the Roots of Phaseolus vulgaris. Interaction of the Inhibitors of Polyamine Biosynthesis with Putrescine in Growth and Polyamine Biosynthesis","volume":"28","year":"1987","journal-title":"Plant Cell Physiol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Hashem, A.M., Moore, S., Chen, S., Hu, C., Zhao, Q., Elesawi, I.E., Feng, Y., Topping, J.F., Liu, J., and Lindsey, K. (2021). Putrescine Depletion Affects Arabidopsis Root Meristem Size by Modulating Auxin and Cytokinin Signaling and ROS Accumulation. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22084094"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Rangel, D., Ch\u00e1vez-Mart\u00ednez, A.I., Rodr\u00edguez-Hern\u00e1ndez, A.A., Maruri-L\u00f3pez, I., Urano, K., Shinozaki, K., and Jim\u00e9nez-Bremont, J.F. (2016). Simultaneous Silencing of Two Arginine Decarboxylase Genes Alters Development in Arabidopsis. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00300"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s11738-000-0002-9","article-title":"Changes in Free Polyamine Level and Di- and Polyamine Oxidase Activity in Cucumber Roots under Allelochemical Stress Conditions","volume":"22","author":"Politycka","year":"2000","journal-title":"Acta Physiol. Plant."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1146\/annurev-arplant-070623-110056","article-title":"Polyamines: Their Role in Plant Development and Stress","volume":"75","year":"2024","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Minocha, R., Majumdar, R., and Minocha, S.C. (2014). Polyamines and Abiotic Stress in Plants: A Complex Relationship. Front. Plant Sci., 5.","DOI":"10.3389\/fpls.2014.00175"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"101253","DOI":"10.1016\/j.stress.2026.101253","article-title":"Polyamine Defense Shield of Plants against Biotic and Abiotic Stress","volume":"20","author":"Nawaz","year":"2026","journal-title":"Plant Stress"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jplph.2009.07.002","article-title":"Cinnamic Acid-Inhibited Ribulose-1,5-Bisphosphate Carboxylase Activity Is Mediated through Decreased Spermine and Changes in the Ratio of Polyamines in Cowpea","volume":"167","author":"Huang","year":"2010","journal-title":"J. Plant Physiol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/0048-3575(91)90136-A","article-title":"Is Polyamine Biosynthesis a Possible Site of Action of Cinmethylin and Artemisinin?","volume":"39","author":"DiTomaso","year":"1991","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.niox.2016.10.008","article-title":"Meta-Tyrosine Induces Modification of Reactive Nitrogen Species Level, Protein Nitration and Nitrosoglutathione Reductase in Tomato Roots","volume":"68","author":"Krasuska","year":"2017","journal-title":"Nitric Oxide"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/17\/1541\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:29:04Z","timestamp":1788524944000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/17\/1541"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":75,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["biology15171541"],"URL":"https:\/\/doi.org\/10.3390\/biology15171541","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]}}}