{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T09:11:12Z","timestamp":1788772272909,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:00:00Z","timestamp":1788566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2401001"],"award-info":[{"award-number":["2023YFD2401001"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key R & D Program of Shandong Province, China","award":["2025LZGC040"],"award-info":[{"award-number":["2025LZGC040"]}]},{"name":"Key R & D Program of Shandong Province, China","award":["2024CXPT071-3"],"award-info":[{"award-number":["2024CXPT071-3"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["2023TD50"],"award-info":[{"award-number":["2023TD50"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods","award":["BRESG-JB202513"],"award-info":[{"award-number":["BRESG-JB202513"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>The euryhaline shrimp Exopalaemon carinicauda is a valuable model for studying osmoregulation, yet the developmental establishment and spatial localization of its key ion-transport sites remain unclear. This study investigated the spatiotemporal expression of Na+\/K+-ATPase (EcNKA), cytoplasmic carbonic anhydrase (EcCAc), and glycosylphosphatidylinositol-linked carbonic anhydrase (EcCAg) during early ontogeny and their subcellular responses to salinity and alkalinity stress in juvenile gills of E. carinicauda. Quantitative real-time PCR was used to detect the changes in mRNA expression of the three genes at different developmental stages, and immunohistochemistry was employed to determine the spatial localization of the corresponding three proteins. EcNKA and EcCAg mRNA expression peaked at zoea I, while EcCAc peaked at zoea III\u2013IV. Immunostaining demonstrated a wide distribution of the three proteins across multiple tissues in early larvae, with a later restriction primarily to the gill and intestine. In gills, the EcNKA protein localized to septal cells, whereas EcCAc and EcCAg were located in pillar cells with distinct membrane polarities. Under low-salinity and high-alkalinity stress, transcriptional changes and protein-level responses of EcCAc and EcCAg were discordant. In addition, the protein location of EcCAc exhibited a translocation from the apical to the basolateral membrane, while EcCAg showed apical concentration under stress. These findings provide new insights into the ontogenetic establishment and subcellular plasticity of key ion-transport sites in E. carinicauda and help us to understand how euryhaline shrimp integrate developmental programming and saline\u2013alkaline stress responses at both the mRNA and protein levels.<\/jats:p>","DOI":"10.3390\/biology15171554","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T08:07:06Z","timestamp":1788768426000},"page":"1554","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ontogeny and Stress-Induced Plasticity of Osmoregulatory Machinery in Exopalaemon carinicauda: Distinct Spatiotemporal Localization of Na+\/K+-ATPase and Carbonic Anhydrase Isoforms"],"prefix":"10.3390","volume":"15","author":[{"given":"Qianqian","family":"Ge","sequence":"first","affiliation":[{"name":"Laoshan National Laboratory, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2292-6528","authenticated-orcid":false,"given":"Jiajia","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Laoshan National Laboratory, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9547-8191","authenticated-orcid":false,"given":"Jitao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s13131-020-1582-4","article-title":"Effects of acute salinity stress on the survival and prophenoloxidase system of Exopalaemon carinicauda","volume":"39","author":"Ge","year":"2020","journal-title":"Acta Oceanol. Sin."},{"key":"ref_2","first-page":"101340","article-title":"Integrated analysis of transcriptome and metabolome reveals chronic low salinity stress responses in the muscle of Exopalaemon carinicauda","volume":"52","author":"Wang","year":"2024","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s12192-019-00987-z","article-title":"Characterization, functional analysis, and expression levels of three carbonic anhydrases in response to pH and saline-alkaline stresses in the ridgetail white prawn Exopalaemon carinicauda","volume":"24","author":"Ge","year":"2019","journal-title":"Cell Stress Chaperones"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tang, S., Wang, J., Yan, K., Yu, Z., and Li, J. (2025). Genome-wide identification of solute carrier family 12 and functional characterization of its role in saline\u2013alkaline stress acclimation in the ridgetail white shrimp Exopalaemon carinicauda. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26178339"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1007\/s00360-012-0665-8","article-title":"Evolution of osmoregulatory patterns and gill ion transport mechanisms in the decapod Crustacea: A review","volume":"182","author":"McNamara","year":"2012","journal-title":"J. Comp. Physiol. Part B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10750-017-3232-1","article-title":"Homeostatic responses of crustaceans to salinity changes","volume":"799","author":"Thabet","year":"2017","journal-title":"Hydrobiologia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"111038","DOI":"10.1016\/j.cbpb.2024.111038","article-title":"Kinetic properties of gill (Na+, K+)-ATPase in the Pacific whiteleg shrimp Penaeus vannamei (Decapoda, Penaeidae)","volume":"275","author":"Moraes","year":"2025","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1021\/acs.biochem.6b01243","article-title":"Modulation of the Na+, K+-ATPase by magnesium ions","volume":"56","author":"Apell","year":"2017","journal-title":"Biochemistry"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s00227-021-03882-3","article-title":"Osmotic and ionic regulation, and kinetic characteristics of a posterior gill (Na+, K+)-ATPase from the blue crab Callinectes danae on acclimation to salinity challenge","volume":"168","author":"Leone","year":"2021","journal-title":"Mar. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S1095-6433(03)00064-3","article-title":"Na+\/K+-ATPase in gills of aquatic crustacea","volume":"135","author":"Lucu","year":"2003","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101577","DOI":"10.1016\/j.aqrep.2023.101577","article-title":"Effects of high-salinity on the expression of aquaporins and ion transport-related genes in Chinese shrimp (Fenneropenaeus chinensis)","volume":"30","author":"Deng","year":"2023","journal-title":"Aquac. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s00441-015-2351-0","article-title":"Immunolocalization and expression of Na+\/K+-ATPase in embryos, early larval stages and adults of the freshwater shrimp Palaemonetes argentinus (Decapoda, Caridea, Palaemonidae)","volume":"364","author":"Ituarte","year":"2016","journal-title":"Cell Tissue Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1242\/jeb.204.5.991","article-title":"Environmentally mediated carbonic anhydrase induction in the gills of euryhaline crustaceans","volume":"204","author":"Henry","year":"2001","journal-title":"J. Exp. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.aquaculture.2015.04.038","article-title":"Cloning and expression analysis of two carbonic anhydrase genes in white shrimp Litopenaeus vannamei, induced by pH and salinity stresses","volume":"448","author":"Liu","year":"2015","journal-title":"Aquaculture"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.gene.2015.03.074","article-title":"Analysis, characterisation and expression of gill-expressed carbonic anhydrase genes in the freshwater crayfish Cherax quadricarinatus","volume":"564","author":"Ali","year":"2015","journal-title":"Gene"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.gene.2015.10.049","article-title":"Molecular cloning and sequence analysis of two carbonic anhydrase in the swimming crab Portunus trituberculatus and its expression in response to salinity and pH stress","volume":"576","author":"Pan","year":"2016","journal-title":"Gene"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.fsi.2014.10.043","article-title":"The roles of Na+\/K+-ATPase \u03b1-subunit gene from the ridgetail white prawn Exopalaemon carinicauda in response to salinity stresses","volume":"42","author":"Li","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1080\/07924259.1998.9652630","article-title":"Ontogeny of osmoregulation in crustaceans: A review","volume":"33","author":"Charmantier","year":"1998","journal-title":"Invertebr. Reprod. Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"169","DOI":"10.3354\/meps329169","article-title":"Osmoregulation and immunolocalization of Na+\/K+-ATPase during the ontogeny of the mitten crab Eriocheir sinensis (Decapoda, Grapsoidea)","volume":"329","author":"Cieluch","year":"2007","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0022-0981(88)90158-X","article-title":"Relationship between osmoregulation, Na+, K+-ATPase activity and carbonic-anhydrase activity in larvae and postlarva of Homarus gammarus (L) (crustacea, decapoda)","volume":"115","author":"Thuet","year":"1988","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/0300-9629(91)90495-X","article-title":"Na+-K+ ATPase and carbonic-anhydrase activities in larvae, postlarva and adults of the shrimp Penaeus japonicus (decapoda, penaeidea)","volume":"100","author":"Bouaricha","year":"1991","journal-title":"Comp. Biochem. Physiol. Part A Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"114902","DOI":"10.1016\/j.ecoenv.2023.114902","article-title":"Effect of high alkalinity on shrimp gills: Histopathological alternations and cell specific responses","volume":"256","author":"Ge","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_23","first-page":"315","article-title":"Single-cell atlas of Exopalaemon carinicauda gills provides insights into pillar cell-mediated responses to low-salinity stress in crustaceans","volume":"47","author":"Ge","year":"2026","journal-title":"Zool. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.ijbiomac.2019.02.098","article-title":"Cloning and characterisation of Na+\/K+-ATPase and carbonic anhydrase from oriental river prawn Macrobrachium nipponense","volume":"129","author":"Huang","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4623","DOI":"10.1242\/jeb.01333","article-title":"Ion-motive ATPases and active, transbranchial NaCl uptake in the red freshwater crab, Dilocarcinus pagei (Decapoda, Trichodactylidae)","volume":"207","author":"Weihrauch","year":"2004","journal-title":"J. Exp. Biol."},{"key":"ref_26","first-page":"1003","article-title":"Cloning, expression and stability analysis of the reference gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Exopalaemon carinicauda","volume":"24","author":"Xue","year":"2017","journal-title":"J. Fish. Sci. China"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.cbpb.2015.12.007","article-title":"Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae): Deciphering the role of the Na+\/K+-ATPase","volume":"196","author":"Pham","year":"2016","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"29","DOI":"10.2307\/1542034","article-title":"Ontogeny of osmoregulatory structures in the shrimp Penaeus japonicus (crustacea, decapoda)","volume":"186","author":"Bouaricha","year":"1994","journal-title":"Biol. Bull."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1177\/002215540104900809","article-title":"Immunolocalization of Na+, K+-ATPase in the branchial cavity during the early development of the European lobster Homarus gammarus (Crustacea, Decapoda)","volume":"49","author":"Lignot","year":"2001","journal-title":"J. Histochem. Cytochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00441-013-1622-x","article-title":"Adaptation to freshwater in the palaemonid shrimp Macrobrachium amazonicum: Comparative ontogeny of osmoregulatory organs","volume":"353","author":"Boulo","year":"2013","journal-title":"Cell Tissue Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/BF02367901","article-title":"Physiological and biochemical-changes during the larval development of a brachyuran crab reared under constant conditions in the laboratory","volume":"43","author":"Anger","year":"1989","journal-title":"Helgol. Meeresunters."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0034-5687(85)90089-1","article-title":"Facilitation of CO2 excretion by carbonic-anhydrase located on the surface of the basal membrane of crab gill epithelium","volume":"62","author":"Burnett","year":"1985","journal-title":"Respir. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.1444-2906.2008.01550.x","article-title":"Carbonic anhydrase and Na+\/K+-ATPase activities at different molting stages of the giant freshwater prawn Macrobrachium rosenbergii","volume":"74","author":"Jasmani","year":"2008","journal-title":"Fish. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1242\/jeb.00759","article-title":"Ontogeny of osmoregulatory structures and functions in the green crab Carcinus maenas (Crustacea, Decapoda)","volume":"207","author":"Cieluch","year":"2004","journal-title":"J. Exp. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1086\/432856","article-title":"Osmoregulation, immunolocalization of Na+\/K+-ATPase, and ultrastructure of branchial epithelia in the developing brown shrimp, Crangon crangon (Decapoda, Caridea)","volume":"78","author":"Cieluch","year":"2005","journal-title":"Physiol. Biochem. Zool."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2320","DOI":"10.1242\/jeb.005041","article-title":"Salinity-stimulated changes in expression and activity of two carbonic anhydrase isoforms in the blue crab Callinectes sapidus","volume":"210","author":"Serrano","year":"2007","journal-title":"J. Exp. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Henry, R.P., Lucu, C., Onken, H., and Weihrauch, D. (2012). Multiple functions of the crustacean gill: Osmotic\/ionic regulation, acid-base balance, ammonia excretion, and bioaccumulation of toxic metals. Front. Physiol., 3.","DOI":"10.3389\/fphys.2012.00431"},{"key":"ref_38","first-page":"414","article-title":"Immunocytochemical localization of V-H+-ATPase, Na+\/K+-ATPase, and carbonic anhydrase in gill lamellae of adult freshwater euryhaline shrimp Macrobrachium acanthurus (Decapoda, Palaemonidae)","volume":"323","author":"Maraschi","year":"2015","journal-title":"J. Exp. Zool. Part A Ecol. Integr. Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.cbpa.2007.05.008","article-title":"A structure-function analysis of ion transport in crustacean gills and excretory organs","volume":"151","author":"Freire","year":"2008","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1242\/jeb.00907","article-title":"The mechanism of sodium chloride uptake in hyperregulating aquatic animals","volume":"207","author":"Kirschner","year":"2004","journal-title":"J. Exp. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1111\/j.1748-1716.2010.02186.x","article-title":"Freshwater fish gill ion transport: August krogh to morpholinos and microprobes","volume":"202","author":"Evans","year":"2011","journal-title":"Acta Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"R1033","DOI":"10.1152\/ajpregu.00516.2007","article-title":"Regulation of ion transport by pH and HCO3\u2212 in isolated gills of the crab Neohelice (Chasmagnathus) granulata","volume":"294","author":"Tresguerres","year":"2008","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/sj.ki.5002020","article-title":"The role of carbonic anhydrases in renal physiology","volume":"71","author":"Purkerson","year":"2007","journal-title":"Kidney Int."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/BF00492602","article-title":"Identification and immunocytochemical localization of 2 different carbonic-anhydrase isoenzymes in teleostean fish erythrocytes and gill epithelia","volume":"89","author":"Rahim","year":"1988","journal-title":"Histochemistry"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"110507","DOI":"10.1016\/j.cbpb.2020.110507","article-title":"Osmotic and ionic regulation, and modulation by protein kinases, FXYD2 peptide and ATP of gill (Na+, K+)-ATPase activity, in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae)","volume":"250","author":"Leone","year":"2020","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.abb.2010.09.017","article-title":"Regulatory phosphorylation of FXYD2 by PKC and cross interactions between FXYD2, plasmalemmal CA-ATPase and Na+, K+-ATPase","volume":"505","author":"Cortes","year":"2011","journal-title":"Arch. Biochem. Biophys."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/17\/1554\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T08:45:49Z","timestamp":1788770749000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/17\/1554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,5]]},"references-count":46,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["biology15171554"],"URL":"https:\/\/doi.org\/10.3390\/biology15171554","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,5]]}}}