{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:59:47Z","timestamp":1792789187588,"version":"4.5.0"},"reference-count":50,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanxi Provincial Modern Agricultural Industrial Technology System Construction Project","award":["2024CYJSTX10-04"],"award-info":[{"award-number":["2024CYJSTX10-04"]}]},{"name":"Shanxi Provincial Modern Agricultural Industrial Technology System Construction Project","award":["2025CYJSTX10-4"],"award-info":[{"award-number":["2025CYJSTX10-4"]}]},{"name":"Shanxi Province Modern Agricultural Industrial Technology System","award":["2026CYJSTX18-18"],"award-info":[{"award-number":["2026CYJSTX18-18"]}]},{"name":"Shanxi Agricultural University","award":["CXGC2026060"],"award-info":[{"award-number":["CXGC2026060"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"crossref"}]},{"award":["CXGC2026060"],"award-info":[{"award-number":["CXGC2026060"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Grain fat content contributes to nutritional quality in foxtail millet, but its genetic basis remains incompletely understood. We evaluated a 256-line recombinant inbred population derived from Jingu21 and Chuang29 in two environments. Among the individual environments, qFC5 was detected only in Sanya (2022_SY; LOD 5.86; phenotypic variance explained: 9.24%), exceeding the LOD threshold of 3.58. Across-environment mapping using best linear unbiased estimates identified an overlapping chromosome 5 region (LOD: 5.99; phenotypic variance explained: 7.67%), exceeding the threshold of 4.14 from 1000 permutations. Among 248 annotated genes in the 1.80 Mb Sanya interval, Arabidopsis and rice homology, functional annotation and expression information supported prioritization of Seita.5G394800.1 (SiMGT2), a predicted MGT\/MRS2 magnesium transporter. Six SiMGT family members were identified. In the parental panicle dataset, SiMGT2 had a higher mean expression in Jingu21 at three sampling stages; only the middle-stage difference remained significant after correction across 18 comparisons. In 534 phenotyped accessions, H1 had a higher grain fat content than H2, but not H3; these comparisons were not corrected for population structure or kinship. The results prioritize SiMGT2 as a candidate associated with grain fat content, with environment-dependent QTL support. Causality and the proposed connection with magnesium homeostasis require functional validation.<\/jats:p>","DOI":"10.3390\/biology15181631","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:06:37Z","timestamp":1789549597000},"page":"1631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["QTL-Guided Prioritization of SiMGT2 as a Candidate Gene Associated with Grain Fat Content in Foxtail Millet"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6155-5562","authenticated-orcid":false,"given":"Wei","family":"Zhou","sequence":"first","affiliation":[{"name":"Shanxi Institute for Functional Food, HouJi Laboratory of Shanxi Province, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Hongyuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanxi Center for Inspection and Testing, Shanxi Institute of Standardization and Metrology Technology, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Hui","family":"Zhi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Resources and Breeding, Key Laboratory Grain Crop Genetic Resources Evaluation and Utilization, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Cheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Institute for Functional Food, HouJi Laboratory of Shanxi Province, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Honggang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Institute for Functional Food, HouJi Laboratory of Shanxi Province, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Minghui","family":"Guo","sequence":"additional","affiliation":[{"name":"Shanxi Institute for Functional Food, HouJi Laboratory of Shanxi Province, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.tplants.2020.12.003","article-title":"Foxtail millet: A new model for C4 plants","volume":"26","author":"Peng","year":"2021","journal-title":"Trends Plant Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"16","DOI":"10.15302\/J-FASE-2014011","article-title":"Initiation of Setaria as a model plant","volume":"1","author":"Diao","year":"2014","journal-title":"Front. Agric. Sci. Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1104\/pp.108.129627","article-title":"Foxtail millet: A sequence-driven grass model system","volume":"149","author":"Doust","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.gaost.2022.06.005","article-title":"Influences of four processing methods on main nutritional components of foxtail millet: A review","volume":"5","author":"Yang","year":"2022","journal-title":"Grain Oil Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e11186","DOI":"10.1016\/j.heliyon.2022.e11186","article-title":"Physical, functional, nutritional and antioxidant properties of foxtail millet in Bangladesh","volume":"8","author":"Abedin","year":"2022","journal-title":"Heliyon"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e111","DOI":"10.1002\/efd2.111","article-title":"Nutritional exploration of foxtail millet (Setaria italica) in addressing food security and its utilization trends in food system","volume":"4","author":"Kalsi","year":"2023","journal-title":"eFood"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Garutti, M., Nevola, G., Mazzeo, R., Cucciniello, L., Totaro, F., Bertuzzi, C.A., Caccialanza, R., Pedrazzoli, P., and Puglisi, F. (2022). The impact of cereal grain composition on the health and disease outcomes. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.888974"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Shahidi, F., and Hossain, A. (2022). Role of lipids in food flavor generation. Molecules, 27.","DOI":"10.3390\/molecules27155014"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1038\/ng.2673","article-title":"A haplotype map of genomic variations and genome-wide association studies of agronomic traits in foxtail millet (Setaria italica)","volume":"45","author":"Jia","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5020","DOI":"10.1038\/s41598-019-41602-6","article-title":"Genome-wide association study of major agronomic traits in foxtail millet (Setaria italica L.) using ddRAD sequencing","volume":"9","author":"Jaiswal","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1038\/s41588-023-01423-w","article-title":"A graph-based genome and pan-genome variation of the model plant Setaria","volume":"55","author":"He","year":"2023","journal-title":"Nat. Genet."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s00122-020-03714-w","article-title":"QTL mapping for foxtail millet plant height in multi-environment using an ultra-high density bin map","volume":"134","author":"He","year":"2021","journal-title":"Theor. Appl. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Wei, W., Li, S., Li, P., Yu, K., Fan, G., Wang, Y., Zhao, F., Zhang, X., Feng, X., and Shi, G. (2023). QTL analysis of important agronomic traits and metabolites in foxtail millet (Setaria italica) by RIL population and widely targeted metabolome. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1035906"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhang, L., Ma, K., Zhao, X., Li, Z., Zhang, X., Li, W., Meng, R., Lu, B., and Yuan, X. (2023). Development of a comprehensive quality evaluation system for foxtail millet from different ecological regions. Foods, 12.","DOI":"10.3390\/foods12132545"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ma, K., Zhao, X., Lu, B., Wang, Y., Yue, Z., Zhang, L., Diao, X., and Yuan, X. (2024). Effect of ecological factors on nutritional quality of foxtail millet (Setaria italica L.). Agronomy, 14.","DOI":"10.3390\/agronomy14020387"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"23076","DOI":"10.3390\/ijms160923076","article-title":"Critical issues in the study of magnesium transport systems and magnesium deficiency symptoms in plants","volume":"16","author":"Kobayashi","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kleczkowski, L.A., and Igamberdiev, A.U. (2021). Magnesium signaling in plants. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031159"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.1105\/tpc.010352","article-title":"A novel family of magnesium transport genes in Arabidopsis","volume":"13","author":"Li","year":"2001","journal-title":"Plant Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1093\/pcp\/pct112","article-title":"Expression and functional analysis of the CorA-MRS2-ALR-type magnesium transporter family in rice","volume":"54","author":"Saito","year":"2013","journal-title":"Plant Cell Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1469-8137.2010.03619.x","article-title":"Magnesium transporters, MGT2\/MRS2-1 and MGT3\/MRS2-5, are important for magnesium partitioning within Arabidopsis thaliana mesophyll vacuoles","volume":"190","author":"Conn","year":"2011","journal-title":"New Phytol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.plantsci.2005.08.018","article-title":"A putative magnesium transporter AtMRS2-11 is localized to the plant chloroplast envelope membrane system","volume":"170","author":"Drummond","year":"2006","journal-title":"Plant Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Chen, Y., Zhang, W., Zhao, F., Liu, G., Zhao, D., Xu, J., Wang, X., Zong, X., Zhang, J., and Ji, X. (2025). Genome-Wide Identification, Phylogeny and Expression Analysis of the Magnesium Release Gene Family in Wheat (Triticum aestivum L.). Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47110882"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.plaphy.2019.01.017","article-title":"Molecular identification of the magnesium transport gene family in Brassica napus","volume":"136","author":"Zhang","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Liu, W., Khan, S., Tong, M., Hu, H., Yin, L., and Huang, J. (2023). Identification and Expression of the CorA\/MRS2\/ALR Type Magnesium Transporters in Tomato. Plants, 12.","DOI":"10.3390\/plants12132512"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhou, W., Zhi, H., Liu, B., He, Q., Sun, Y., Wang, L., Xie, H., Gao, M., Yang, Q., and Wang, H. (J. Integr. Agric., 2026). QTL dissection of yield-related traits in foxtail millet using cross-ecotype RIL, J. Integr. Agric., in press.","DOI":"10.1016\/j.jia.2026.05.006"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"D412","DOI":"10.1093\/nar\/gkaa913","article-title":"Pfam: The protein families database in 2021","volume":"49","author":"Mistry","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkr367","article-title":"HMMER web server: Interactive sequence similarity searching","volume":"39","author":"Finn","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1002\/dvg.22877","article-title":"The Arabidopsis information resource: Making and mining the \u201cgold standard\u201d annotated reference plant genome","volume":"53","author":"Berardini","year":"2015","journal-title":"Genesis"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"D1178","DOI":"10.1093\/nar\/gkr944","article-title":"Phytozome: A comparative platform for green plant genomics","volume":"40","author":"Goodstein","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Hallgren, J., Tsirigos, K.D., Pedersen, M.D., Almagro Armenteros, J.J., Marcatili, P., Nielsen, H., Krogh, A., and Winther, O. (2022). DeepTMHMM predicts alpha and beta transmembrane proteins using deep neural networks. bioRxiv.","DOI":"10.1101\/2022.04.08.487609"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2016). Data analysis. ggplot2: Elegant Graphics for Data Analysis, Springer.","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4","DOI":"10.32614\/RJ-2015-001","article-title":"Peptides: A package for data mining of antimicrobial peptides","volume":"7","author":"Osorio","year":"2015","journal-title":"R J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1093\/bioinformatics\/11.6.681","article-title":"SOPMA: Significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments","volume":"11","author":"Geourjon","year":"1995","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"W585","DOI":"10.1093\/nar\/gkm259","article-title":"WoLF PSORT: Protein localization predictor","volume":"35","author":"Horton","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3997","DOI":"10.1093\/bioinformatics\/btv494","article-title":"msa: An R package for multiple sequence alignment","volume":"31","author":"Bodenhofer","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1101\/gr.073585.107","article-title":"EnsemblCompara GeneTrees: Complete, duplication-aware phylogenetic trees in vertebrates","volume":"19","author":"Vilella","year":"2009","journal-title":"Genome Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1093\/bioinformatics\/8.3.275","article-title":"The rapid generation of mutation data matrices from protein sequences","volume":"8","author":"Jones","year":"1992","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1093\/molbev\/msx281","article-title":"UFBoot2: Improving the ultrafast bootstrap approximation","volume":"35","author":"Hoang","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W39","DOI":"10.1093\/nar\/gkv416","article-title":"The MEME suite","volume":"43","author":"Bailey","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1093\/bioinformatics\/btu817","article-title":"GSDS 2.0: An upgraded gene feature visualization server","volume":"31","author":"Hu","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e49","DOI":"10.1093\/nar\/gkr1293","article-title":"MCScanX: A toolkit for detection and evolutionary analysis of gene synteny and collinearity","volume":"40","author":"Wang","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S1672-0229(10)60008-3","article-title":"KaKs_Calculator 2.0: A toolkit incorporating gamma-series methods and sliding window strategies","volume":"8","author":"Wang","year":"2010","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1093\/oxfordjournals.molbev.a026236","article-title":"Estimating synonymous and nonsynonymous substitution rates under realistic evolutionary models","volume":"17","author":"Yang","year":"2000","journal-title":"Mol. Biol. Evol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1007\/s00425-018-2849-2","article-title":"Genome-wide identification, classification, and expression of phytocyanins in Populus trichocarpa","volume":"247","author":"Luo","year":"2018","journal-title":"Planta"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.molp.2023.12.017","article-title":"A complete reference genome assembly for foxtail millet and Setaria-db, a comprehensive database for Setaria","volume":"17","author":"He","year":"2024","journal-title":"Mol. Plant"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1093\/pcp\/pcw064","article-title":"Identification, and functional and expression analyses of the CorA\/MRS2\/MGT-type magnesium transporter family in maize","volume":"57","author":"Li","year":"2016","journal-title":"Plant Cell Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"108082","DOI":"10.1016\/j.fcr.2021.108082","article-title":"Effect of magnesium fertilization on seed yield, seed quality, carbon assimilation and nutrient uptake of rapeseed plants","volume":"264","author":"Geng","year":"2021","journal-title":"Field Crops Res."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/18\/1631\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:21:14Z","timestamp":1789705274000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/18\/1631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":50,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["biology15181631"],"URL":"https:\/\/doi.org\/10.3390\/biology15181631","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]}}}