{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:56:02Z","timestamp":1792788962419,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Earmarked Fund for China Agriculture Research System","award":["CARS-44-KXJ7"],"award-info":[{"award-number":["CARS-44-KXJ7"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"crossref"}]},{"award":["CARS-44-KXJ7"],"award-info":[{"award-number":["CARS-44-KXJ7"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Project of NINGBO Leading Medical & Health Discipline","award":["2026-Z13"],"award-info":[{"award-number":["2026-Z13"]}]},{"name":"Master Supervisor Team Fund of Fujian Agriculture and Forestry University","id":[{"id":"https:\/\/ror.org\/04kx2sy84","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/04kx2sy84","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Special Fund for Scientific and Technological Innovation of Fujian Agriculture and Forestry University","award":["KFb22060XA"],"award-info":[{"award-number":["KFb22060XA"]}],"id":[{"id":"https:\/\/ror.org\/04kx2sy84","id-type":"ROR","asserted-by":"crossref"}]},{"award":["KFb22060XA"],"award-info":[{"award-number":["KFb22060XA"]}],"id":[{"id":"https:\/\/ror.org\/04kx2sy84","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Breast cancer remains a major clinical challenge because of its molecular heterogeneity, therapeutic resistance, and disease recurrence. Melittin, the principal bioactive peptide of honeybee venom, exhibits antitumor activity in multiple experimental models; however, its early proteome-wide effects in breast cancer cells remain incompletely characterized. In this study, E0771 murine mammary carcinoma cells were exposed to melittin for 15 min. Colony formation and CCK-8 assays using 0\u20138 \u03bcg\/mL melittin showed concentration-dependent reductions in clonogenic capacity and cell viability. For DIA-based quantitative proteomic profiling, E0771 cells were treated with 4 \u03bcg\/mL melittin for 15 min. Among 9748 quantified protein entries, 339 proteins met the exploratory criteria for candidate differential abundance, including 114 up-regulated and 225 down-regulated candidates. GO and KEGG analyses associated these candidate proteins with cell junctions, cytoskeletal organization, protein kinase-related functions, metabolic processes, and cell-death-associated pathways. A STRING-derived association network containing 132 nodes and 216 edges further prioritized CASP8, RPS27A, ANXA5, and PIK3CA as relatively high-connectivity candidates. Collectively, these findings describe early proteomic responses to short-term melittin exposure and provide candidate proteins and biological processes for subsequent mechanistic validation rather than establishing causal molecular mechanisms.<\/jats:p>","DOI":"10.3390\/biology15191727","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:38:16Z","timestamp":1790847496000},"page":"1727","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DIA Quantitative Proteomic Analysis Reveals Early Proteomic Responses to Honeybee-Derived Melittin in Murine Breast Cancer Cells"],"prefix":"10.3390","volume":"15","author":[{"given":"Junling","family":"Zhu","sequence":"first","affiliation":[{"name":"Ningbo No. 2 Hospital, Wenzhou Medical University, Ningbo 315000, 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