{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:01:32Z","timestamp":1791187292458,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T00:00:00Z","timestamp":1364342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>The most familiar icy environments, seasonal lake and stream ice, have received little microbiological study. Bacteria and Eukarya dominated the microbial assemblage within the seasonal ice of Miquelon Lake, a shallow saline lake in Alberta, Canada. The bacterial assemblages were moderately diverse and did not vary with either ice depth or time. The closest relatives of the bacterial sequences from the ice included Actinobacteria, Bacteroidetes, Proteobacteria, Verrucomicrobia, and Cyanobacteria. The eukaryotic assemblages were less conserved and had very low diversity. Green algae relatives dominated the eukaryotic gene sequences; however, a copepod and cercozoan were also identified, possibly indicating the presence of complete microbial loop. The persistence of a chlorophyll a peak at 25\u201330 cm below the ice surface, despite ice migration and brine flushing, indicated possible biological activity within the ice. This is the first study of the composition, diversity, and stability of seasonal lake ice.<\/jats:p>","DOI":"10.3390\/biology2020514","type":"journal-article","created":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T13:02:08Z","timestamp":1364389328000},"page":"514-532","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Composition, Diversity, and Stability of Microbial Assemblages in Seasonal Lake Ice, Miquelon Lake, Central Alberta"],"prefix":"10.3390","volume":"2","author":[{"given":"Anna","family":"Bramucci","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, Edmonton, AB T6G2E9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukkyun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, Edmonton, AB T6G2E9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Beckers","sequence":"additional","affiliation":[{"name":"Department of Earth and Atmospheric Sciences, University of Alberta, Edmonton, AB T6G2E9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Haas","sequence":"additional","affiliation":[{"name":"Department of Earth and Atmospheric Sciences, University of Alberta, Edmonton, AB T6G2E9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Lanoil","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, Edmonton, AB T6G2E9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1111\/j.1462-2920.2005.00781.x","article-title":"Recent advances in sea-ice microbiology","volume":"7","author":"Mock","year":"2005","journal-title":"Environ. 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