{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T05:32:55Z","timestamp":1780378375767,"version":"3.54.1"},"reference-count":65,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANR-2012","award":["ANR-12-BSV5\u22120016\u221201"],"award-info":[{"award-number":["ANR-12-BSV5\u22120016\u221201"]}]},{"name":"H2020-MSCE-RISE-2015","award":["PANG, No. 690836"],"award-info":[{"award-number":["PANG, No. 690836"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Shear force exerted on uropathogenic Escherichia coli adhering to surfaces makes type-1 fimbriae stretch out like springs to catch on to mannosidic receptors. This mechanism is initiated by a disruption of the quaternary interactions between the lectin and the pilin of the two-domain FimH adhesin and transduces allosterically to the mannose-binding pocket of FimH to increase its affinity. Mannose-specific adhesion of 14 E. coli pathovars was measured under flow, using surface plasmon resonance detection on functionalized graphene-coated gold interfaces. Increasing the shear had important differential consequences on bacterial adhesion. Adherent-invasive E. coli, isolated from the feces and biopsies of Crohn\u2019s disease patients, consistently changed their adhesion behavior less under shear and displayed lower SPR signals, compared to E. coli opportunistically infecting the urinary tract, intestines or loci of knee and hip prostheses. We exemplified this further with the extreme behaviors of the reference strains UTI89 and LF82. Whereas their FimA major pilins have identical sequences, FimH of LF82 E. coli is marked by the Thr158Pro mutation. Positioned in the inter-domain region known to carry hot spots of mutations in E. coli pathotypes, residue 158 is indicated to play a structural role in the allosteric regulation of type-1 fimbriae-mediated bacterial adhesion.<\/jats:p>","DOI":"10.3390\/biology5020014","type":"journal-article","created":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T10:31:20Z","timestamp":1459506680000},"page":"14","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Differentiation of Crohn\u2019s Disease-Associated Isolates from Other Pathogenic Escherichia coli by Fimbrial Adhesion under Shear Force"],"prefix":"10.3390","volume":"5","author":[{"given":"Sabine","family":"Szunerits","sequence":"first","affiliation":[{"name":"Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Universit\u00e9 Lille 1, Avenue Poincar\u00e9-BP 60069, 59652 Villeneuve d\u2019Ascq, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleksandr","family":"Zagorodko","sequence":"additional","affiliation":[{"name":"Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Universit\u00e9 Lille 1, Avenue Poincar\u00e9-BP 60069, 59652 Villeneuve d\u2019Ascq, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Cogez","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR 8576 CNRS, Universit\u00e9 de Lille, 59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetiana","family":"Dumych","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR 8576 CNRS, Universit\u00e9 de Lille, 59000 Lille, France"},{"name":"Department of Cytology, Histology, Embryology, Danylo Halytsky Lviv National Medical University, Pekarska Str 69, 79010 Lviv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibaut","family":"Chalopin","sequence":"additional","affiliation":[{"name":"Chimie Et Interdisciplinarit\u00e9, Synth\u00e8se, Analyse, Mod\u00e9lisation (CEISAM), UMR 6230 CNRS, LUNAM Universit\u00e9, 44322 Nantes Cedex 3, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitri","family":"Alvarez Dorta","sequence":"additional","affiliation":[{"name":"Chimie Et Interdisciplinarit\u00e9, Synth\u00e8se, Analyse, Mod\u00e9lisation (CEISAM), UMR 6230 CNRS, LUNAM Universit\u00e9, 44322 Nantes Cedex 3, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Sivignon","sequence":"additional","affiliation":[{"name":"M2iSH, Microbes, Intestin, Inflammation and Susceptibility of the Host , UMR1071 Inserm\/Universit\u00e9 d\u2019Auvergne, USC INRA 2018, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Barnich","sequence":"additional","affiliation":[{"name":"M2iSH, Microbes, Intestin, Inflammation and Susceptibility of the Host , UMR1071 Inserm\/Universit\u00e9 d\u2019Auvergne, USC INRA 2018, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Harduin-Lepers","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR 8576 CNRS, Universit\u00e9 de Lille, 59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iban","family":"Larroulet","sequence":"additional","affiliation":[{"name":"SENSIA SL, Poligono Aranguren, 9, Apdo, Correos 171, 20180 Oiartzun, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aritz","family":"Yanguas Serrano","sequence":"additional","affiliation":[{"name":"SENSIA SL, Poligono Aranguren, 9, Apdo, Correos 171, 20180 Oiartzun, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aloysius","family":"Siriwardena","sequence":"additional","affiliation":[{"name":"Glycochimie des Antimicrobiens et des Agroressources, UMR 7378 CNRS, Universit\u00e9 de Picardie Jules Verne, 80000 Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaia","family":"Pesquera","sequence":"additional","affiliation":[{"name":"Graphenea S.A., Tolosa Hiribidea, 76, E-20018 Donostia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaia","family":"Zurutuza","sequence":"additional","affiliation":[{"name":"Graphenea S.A., Tolosa Hiribidea, 76, E-20018 Donostia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Gouin","sequence":"additional","affiliation":[{"name":"Chimie Et Interdisciplinarit\u00e9, Synth\u00e8se, Analyse, Mod\u00e9lisation (CEISAM), UMR 6230 CNRS, LUNAM Universit\u00e9, 44322 Nantes Cedex 3, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabah","family":"Boukherroub","sequence":"additional","affiliation":[{"name":"Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Universit\u00e9 Lille 1, Avenue Poincar\u00e9-BP 60069, 59652 Villeneuve d\u2019Ascq, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Bouckaert","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR 8576 CNRS, Universit\u00e9 de Lille, 59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/128_2008_13","article-title":"Structure, function, and assembly of type-1 fimbriae","volume":"288","author":"Knight","year":"2009","journal-title":"Top. Curr. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1073\/pnas.92.6.2081","article-title":"FimH adhesin of type-1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae","volume":"92","author":"Jones","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8922","DOI":"10.1073\/pnas.95.15.8922","article-title":"Pathogenic adaptation of Escherichia coli by natural variation of the FimH adhesin","volume":"95","author":"Sokurenko","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.cell.2010.03.038","article-title":"Structural basis for mechanical force regulation of the adhesin FimH via finger trap-like beta sheet twisting","volume":"141","author":"Aprikian","year":"2010","journal-title":"Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"23437","DOI":"10.1074\/jbc.M702037200","article-title":"Interdomain interaction in the FimH adhesin of Escherichia coli regulates the affinity to mannose","volume":"282","author":"Aprikian","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/j.1365-2958.2004.04415.x","article-title":"Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin","volume":"55","author":"Bouckaert","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1126\/science.285.5430.1061","article-title":"X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli","volume":"285","author":"Choudhury","year":"1999","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1046\/j.1365-2958.2002.02915.x","article-title":"Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection","volume":"44","author":"Hung","year":"2002","journal-title":"Mol. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Aprikian, P., Interlandi, G., Kidd, B.A., Le Trong, I., Tchesnokova, V., Yakovenko, O., Whitfield, M.J., Bullitt, E., Stenkamp, R.E., and Thomas, W.E. (2011). The bacterial fimbrial tip acts as a mechanical force sensor. PLoS. Biol., 9.","DOI":"10.1371\/journal.pbio.1000617"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/S0092-8674(02)00796-1","article-title":"Bacterial adhesion to target cells enhanced by shear force","volume":"109","author":"Thomas","year":"2002","journal-title":"Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1529\/biophysj.105.066548","article-title":"Catch-bond model derived from allostery explains force-activated bacterial adhesion","volume":"90","author":"Thomas","year":"2006","journal-title":"Biophys. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1083\/jcb.200609029","article-title":"For catch bonds, it all hinges on the interdomain region","volume":"174","author":"Thomas","year":"2006","journal-title":"J. Cell Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11596","DOI":"10.1074\/jbc.M707815200","article-title":"Fimh forms catch bonds that are enhanced by mechanical force due to allosteric regulation","volume":"283","author":"Yakovenko","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/j.str.2008.03.012","article-title":"Beyond induced-fit receptor-ligand interactions: Structural changes that can significantly extend bond lifetimes","volume":"16","author":"Nilsson","year":"2008","journal-title":"Structure"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Sauer, M.M., Jakob, R.P., Eras, J., Baday, S., Eris, D., Navarra, G., Berneche, S., Ernst, B., Maier, T., and Glockshuber, R. (2016). Catch-bond mechanism of the bacterial adhesin FimH. Nat. Commun.","DOI":"10.1038\/ncomms10738"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.chom.2008.09.005","article-title":"Catch bond mechanism of force-enhanced adhesion: Counter-intuitive, elusive but \u2026 widespread?","volume":"16","author":"Sokurenko","year":"2008","journal-title":"Cell Host Microbe"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1111\/j.1365-2958.2004.04226.x","article-title":"Shear-dependent \u2018stick-and-roll\u2019 adhesion of type-1 fimbriated Escherichia coli","volume":"53","author":"Thomas","year":"2004","journal-title":"Mol. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/CMR.19.1.127-141.2006","article-title":"Microbial adhesion in flow displacement systems","volume":"19","author":"Busscher","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.bpj.2010.11.078","article-title":"Shear stress increases the residence time of adhesion of Pseudomonas aeruginosa","volume":"100","author":"Lecuyer","year":"2011","journal-title":"Biophys. J."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Klinth, J.E., Castelain, M., Uhlin, B.E., and Axner, O. (2012). The influence of ph on the specific adhesion of p piliated Escherichia coli. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0038548"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7823","DOI":"10.1074\/jbc.M707804200","article-title":"Integrin-like allosteric properties of the catch bond-forming fimh adhesin of Escherichia coli","volume":"283","author":"Tchesnokova","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1021\/ac5037704","article-title":"Carbohydrates as new probes for the identification of closely related Escherichia coli strains using surface plasmon resonance imaging","volume":"87","author":"Bulard","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3390\/bios5020276","article-title":"Surface plasmon resonance (SPR) for the evaluation of shear-force-dependent bacterial adhesion","volume":"5","author":"Zagorodko","year":"2015","journal-title":"Biosensors"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5422","DOI":"10.1021\/am405541z","article-title":"Graphene-coated surface plasmon resonance interfaces for studying the interactions between bacteria and surfaces","volume":"6","author":"Subramanian","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3833","DOI":"10.1039\/c2nr31040f","article-title":"Graphene: A versatile nanoplatform for biomedical applications","volume":"4","author":"Zhang","year":"2012","journal-title":"Nanoscale"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s11468-014-9686-3","article-title":"Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces","volume":"9","author":"Penezic","year":"2014","journal-title":"Plasmonics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"11211","DOI":"10.1021\/ac502705n","article-title":"Highly sensitive detection of DNA hybridization on commercialized graphene coated surface plasmon resonance interfaces","volume":"86","author":"Zagorodko","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.bios.2013.06.026","article-title":"Lysozyme detection on aptamer functionalized graphene-coated SPR interfaces","volume":"50","author":"Subramanian","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4670","DOI":"10.1021\/nn200500h","article-title":"Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells","volume":"5","author":"Nayak","year":"2011","journal-title":"ACS Nano"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"8100","DOI":"10.1021\/nn202699t","article-title":"Graphene oxide: A nonspecific enhancer of cellular growth","volume":"5","author":"Ruiz","year":"2011","journal-title":"ACS Nano"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6587","DOI":"10.1021\/nn1018279","article-title":"Behaviors of nih-3t3 fibroblasts on graphene\/carbon nanotubes: Proliferation, focal adhesion, and gene transfection studies","volume":"4","author":"Ryoo","year":"2010","journal-title":"ACS Nano"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Dreux, N., Denizot, J., Martinez-Medina, M., Mellmann, A., Billig, M., Kisiela, D., Chattopadhyay, S., Sokurenko, E., Neut, C., and Gower-Rousseau, C. (2013). Point mutations in FimH adhesin of Crohn\u2019s disease-associated adherent-invasive Escherichia coli enhance intestinal inflammatory response. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003141"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1021\/acs.biomac.5b00413","article-title":"Glycopolymers as antiadhesives of E. coli strains inducing inflammatory bowel diseases","volume":"16","author":"Yan","year":"2015","journal-title":"Biomacromolecules"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4122","DOI":"10.1021\/cm4027896","article-title":"Carbohydrate coatings via aryldiazonium chemistry for surface biomimicry","volume":"25","author":"Jayasundara","year":"2013","journal-title":"Chem. Mater."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1039\/c3nr33826f","article-title":"Glycan-functionalized diamond nanoparticles as potent E. coli anti-adhesives","volume":"5","author":"Barras","year":"2013","journal-title":"Nanoscale"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"22439","DOI":"10.1073\/pnas.0902179106","article-title":"Positive selection identifies an in vivo role for FimH during urinary tract infection in addition to mannose binding","volume":"109","author":"Chen","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1089\/10665270050081478","article-title":"A greedy algorithm for aligning DNA sequences","volume":"7","author":"Zhang","year":"2000","journal-title":"J. Comput. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of COOT","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"ref_39","unstructured":"Delano, W.L. The PyMOL Molecular Graphics System, DeLano Scientific. Version 1.8."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13092","DOI":"10.1073\/pnas.230451697","article-title":"Measuring the forces involved in polyvalent adhesion of uropathogenic Escherichia coli to mannose-presenting surfaces","volume":"97","author":"Liang","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1002\/cmdc.200700063","article-title":"Mannosylated G(0) dendrimers with nanomolar affinities to Escherichia coli FimH","volume":"2","author":"Touaibia","year":"2007","journal-title":"Chem. Med. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1021\/acsami.5b08963","article-title":"Spontaneous protein adsorption on graphene oxide nanosheets allows efficient intracellular vaccine protein delivery","volume":"8","author":"Li","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5386","DOI":"10.1021\/am3013196","article-title":"Preparation of a responsive carbohydrate-coated biointerface based on graphene\/azido-terminated tetrathiafulvalene nanohybrid material","volume":"4","author":"Kaminska","year":"2012","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3407","DOI":"10.1021\/jz101360k","article-title":"Adsorption of aromatic and anti-aromatic systems on graphene through \u03c0\u2212\u03c0 stacking","volume":"1","author":"Hanke","year":"2010","journal-title":"J. Phys. Chem. Lett."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1021\/la802284j","article-title":"Interaction of substituted aromatic compounds with graphene","volume":"25","author":"Rochefort","year":"2008","journal-title":"Langmuir"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1099\/00221287-148-2-597","article-title":"Bacterial deposition in a parallel plate and a stagnation point flow chamber: Microbial adhesion mechanisms depend on the mass transport conditions","volume":"148","author":"Bakker","year":"2002","journal-title":"Microbiology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3122","DOI":"10.1099\/mic.0.2008\/018622-0","article-title":"Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces","volume":"154","author":"Boks","year":"2008","journal-title":"Microbiology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"894","DOI":"10.3390\/biology2030894","article-title":"Structural sampling of glycan interaction profiles reveals mucosal receptors for fimbrial adhesins of enterotoxigenic Escherichia coli","volume":"2","author":"Lonardi","year":"2013","journal-title":"Biology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ab.2007.08.017","article-title":"Extracting kinetic rate constants from surface plasmon resonance array systems","volume":"373","author":"Rich","year":"2008","journal-title":"Anal. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1128\/iai.54.3.613-620.1986","article-title":"Regulation of production of type-1 pili among urinary tract isolates of Escherichia coli","volume":"54","author":"Hultgren","year":"1986","journal-title":"Infect. Immun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5395","DOI":"10.1021\/jm400723n","article-title":"Thiazolylaminomannosides as potent anti-adhesives of type-1 piliated Escherichia coli isolated from Crohn's disease patients","volume":"56","author":"Brument","year":"2013","journal-title":"J. Med. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"11369","DOI":"10.1039\/C5OB01581B","article-title":"Inhibition profiles of mono- and polyvalent FimH antagonists against 10 different Escherichia coli strains","volume":"13","author":"Chalopin","year":"2015","journal-title":"Org. Biomol. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Sivignon, A., Yan, X., Alvarez Dorta, D., Bonnet, R., Bouckaert, J., Fleury, E., Bernard, J., Gouin, S.G., Darfeuille-Michaud, A., and Barnich, N. (2015). Development of heptylmannoside-based glycoconjugate antiadhesive compounds against adherent-invasive Escherichia coli bacteria associated with Crohn\u2019s disease. MBio.","DOI":"10.1128\/mBio.01298-15"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"16656","DOI":"10.1074\/jbc.M511496200","article-title":"Catch bond-mediated adhesion without a shear threshold\u2014Trimannose versus monomannose interactions with the fimh adhesin of Escherichia coli","volume":"281","author":"Nilsson","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1111\/j.1365-2958.2005.04985.x","article-title":"Clonal analysis reveals high rate of structural mutations in fimbrial adhesins of extraintestinal pathogenic Escherichia coli","volume":"59","author":"Weissman","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1016\/j.bpj.2013.03.059","article-title":"The shaft of the type-1 fimbriae regulates an external force to match the FimH catch bond","volume":"104","author":"Zakrisson","year":"2013","journal-title":"Biophys. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1111\/j.1365-2958.2006.05375.x","article-title":"The solution structure of the invasive tip complex from afa\/dr fibrils","volume":"62","author":"Cota","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3848","DOI":"10.1529\/biophysj.106.088989","article-title":"The mechanical properties of E. coli type-1 pili measured by atomic force microscopy techniques","volume":"91","author":"Miller","year":"2006","journal-title":"Biophys. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"15530","DOI":"10.1073\/pnas.1315203110","article-title":"Positively selected FimH residues enhance virulence during urinary tract infection by altering FimH conformation","volume":"110","author":"Schwartz","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/IAI.05169-11","article-title":"Type-1 fimbrial adhesin FimH elicits an immune response that enhances cell adhesion of Escherichia coli","volume":"79","author":"Tchesnokova","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Kisiela, D.I., Avagyan, H., Friend, D., Jalan, A., Gupta, S., Interlandi, G., Liu, Y., Tchesnokova, V., Rodriguez, V.B., and Sumida, J.P. (2015). Inhibition and reversal of microbial attachment by an antibody with parasteric activity against the FimH adhesin of uropathogenic E. coli. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004857"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1111\/j.1365-2958.2006.05352.x","article-title":"The affinity of the FimH fimbrial adhesin is receptor-driven and quasi-independent of escherichia coli pathotypes","volume":"61","author":"Bouckaert","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Wellens, A., Garofalo, C., Nguyen, H., van Gerven, N., Slattegard, R., Hernalsteens, J.P., Wyns, L., Oscarson, S., de Greve, H., and Hultgren, S. (2008). Intervening with urinary tract infections using anti-adhesives based on the crystal structure of the FimH-oligomannose-3 complex. PLoS ONE, 3.","DOI":"10.1371\/annotation\/ea59d179-0a71-4836-86f0-8d375f5df089"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1042\/BST0390349","article-title":"Glycosylation changes as important factors for the susceptibility to urinary tract infection","volume":"39","author":"Taganna","year":"2011","journal-title":"Biochem. Soc. Trans."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.1128\/AEM.72.4.3005-3010.2006","article-title":"Elevated shear stress protects Escherichia coli cells adhering to surfaces via catch bonds from detachment by soluble inhibitors","volume":"72","author":"Nilsson","year":"2006","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/5\/2\/14\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:21:40Z","timestamp":1760210500000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/5\/2\/14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,1]]},"references-count":65,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,6]]}},"alternative-id":["biology5020014"],"URL":"https:\/\/doi.org\/10.3390\/biology5020014","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,1]]}}}