{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:20:15Z","timestamp":1782793215092,"version":"3.54.5"},"reference-count":30,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T00:00:00Z","timestamp":1569456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural   Science Foundation of China; Fundamental Research Funds for the Central   Universities","award":["51507081; KJQN201623"],"award-info":[{"award-number":["51507081; KJQN201623"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 \u03bcs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activity of Rhizoctonia solani. High-voltage pulse power was used to treat the bacteria solution with the pulsed electric field. Untreated Rhizoctonia solani were used as the control group. Transmission electron microscope images were used to analyze the cell wall damage. ANOVA was performed on the experimental results and the fitting degree of the model was good (F&gt;&gt;1). Response surface analysis was used to optimize the parameters based on chitin content and polygalacturonase activity. The optimal treatment conditions were obtained as a pulse voltage amplitude of 25 kV, processing time of 2.54 min, and a pulse width of 34.35 \u03bcs. On this basis, experiments were designed to verify the optimized conditions. The results demonstrated that, under the optimal processing conditions, the damage index of the cell wall of Rhizoctonia solani was 9.59% lower in chitin content and 83.05% lower in polygalacturonase activity compared with those of the control group. All indexes were significantly different (P &lt; 0.001), which is consistent with the parameter optimization results. The results provide a theoretical basis for the pulsed electric field assisted sterilization and reference for the design of plant protection machinery in the latter stage.<\/jats:p>","DOI":"10.3390\/biology8040073","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T03:03:15Z","timestamp":1569553395000},"page":"73","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9115-933X","authenticated-orcid":false,"given":"Xuebin","family":"Feng","sequence":"first","affiliation":[{"name":"College of engineering, Nanjing Agricultural University, Nanjing 210031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peijun","family":"He","sequence":"additional","affiliation":[{"name":"College of engineering, Nanjing Agricultural University, Nanjing 210031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoya","family":"Pan","sequence":"additional","affiliation":[{"name":"College of engineering, Nanjing Agricultural University, Nanjing 210031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Xu","sequence":"additional","affiliation":[{"name":"College of engineering, Nanjing Agricultural University, Nanjing 210031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoshan","family":"Xue","sequence":"additional","affiliation":[{"name":"College of engineering, Nanjing Agricultural University, Nanjing 210031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Yin","sequence":"additional","affiliation":[{"name":"College of engineering, Nanjing Agricultural University, Nanjing 210031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Qian","sequence":"additional","affiliation":[{"name":"College of engineering, Nanjing Agricultural University, Nanjing 210031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/S0261-2194(96)00045-2","article-title":"Development of a formulation of Pseudomonas fluorescens PfALR2 for management of rice sheath blight","volume":"15","author":"Rabindran","year":"1996","journal-title":"Crop Prot."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1007\/s11032-014-0173-5","article-title":"Fine-mapping of qSB-9 TQ, a gene conferring major quantitative resistance to rice sheath blight","volume":"34","author":"Zuo","year":"2014","journal-title":"Mol. 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