{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:10:04Z","timestamp":1792523404734,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Biologia Computacional, No. 3381\/2013"],"award-info":[{"award-number":["Biologia Computacional, No. 3381\/2013"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["Not available."],"award-info":[{"award-number":["Not available."]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Mild cognitive impairment (MCI) and Alzheimer\u2019s Disease (AD) are complex diseases with their molecular architecture not elucidated. APOE, Amyloid Beta Precursor Protein (APP), and Presenilin-1 (PSEN1) are well-known genes associated with both MCI and AD. Recently, epigenetic alterations and dysregulated regulatory elements, such as microRNAs (miRNAs), have been reported associated with neurodegeneration. In this study, differential expression analysis (DEA) was performed for genes and miRNAs based on microarray and RNA-Seq data. Global gene profile of healthy individuals, early and late mild cognitive impairment (EMCI and LMCI, respectively), and AD was obtained from ADNI Cohort. miRNA global profile of healthy individuals and AD patients was extracted from public RNA-Seq data. DEA performed with limma package on ADNI Cohort data highlighted eight differential expressed (DE) genes (AGER, LINC00483, MMP19, CATSPER1, ARFGAP1, GPER1, PHLPP2, TRPM2) (false discovery rate (FDR) p-value &lt; 0.05) between EMCI and LMCI patients. Previous molecular studies showed associations between these genes with dementia and neurological-related pathways. Five dysregulated miRNAs were identified by DEA performed with RNA-Seq data and edgeR (FDR p-value &lt; 0.002). All reported miRNAs in AD interact with the aforementioned genes. Our integrative transcriptomic analysis was able to identify a set of miRNA\u2013gene interactions that may be involved in cognitive and neurodegeneration processes.<\/jats:p>","DOI":"10.3390\/biology9090251","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T09:17:08Z","timestamp":1598606228000},"page":"251","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Differential Expression and miRNA\u2013Gene Interactions in Early and Late Mild Cognitive Impairment"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0598-5115","authenticated-orcid":false,"given":"Leonardo Miranda","family":"Brito","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Humana e M\u00e9dica, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Gen\u00e9tica e Biologia Molecular, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7001-1483","authenticated-orcid":false,"given":"\u00c2ndrea","family":"Ribeiro-dos-Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Humana e M\u00e9dica, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Gen\u00e9tica e Biologia Molecular, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4180-9925","authenticated-orcid":false,"given":"Amanda Ferreira","family":"Vidal","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Humana e M\u00e9dica, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Gen\u00e9tica e Biologia Molecular, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9199-9419","authenticated-orcid":false,"given":"Gilderlanio Santana","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Humana e M\u00e9dica, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Gen\u00e9tica e Biologia Molecular, Instituto de Ci\u00eancas Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0074-7742(09)00409-7","article-title":"Alzheimer\u2019s disease genetics current status and future perspectives","volume":"84","author":"Bertram","year":"2009","journal-title":"Int. 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