{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:10:50Z","timestamp":1790716250562,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["20289\/Z16\/Z"],"award-info":[{"award-number":["20289\/Z16\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013266","name":"Central Laser Facility, Science and Technology Facilities Council","doi-asserted-by":"publisher","award":["17330005"],"award-info":[{"award-number":["17330005"]}],"id":[{"id":"10.13039\/100013266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Chlamydia pneumoniae is a Gram-negative bacterium responsible for a number of human respiratory diseases and linked to some chronic inflammatory diseases. The major outer membrane protein (MOMP) of Chlamydia is a conserved immunologically dominant protein located in the outer membrane, which, together with its surface exposure and abundance, has led to MOMP being the main focus for vaccine and antimicrobial studies in recent decades. MOMP has a major role in the chlamydial outer membrane complex through the formation of intermolecular disulphide bonds, although the exact interactions formed are currently unknown. Here, it is proposed that due to the large number of cysteines available for disulphide bonding, interactions occur between cysteine-rich pockets as opposed to individual residues. Such pockets were identified using a MOMP homology model with a supporting low-resolution (~4 \u00c5) crystal structure. The localisation of MOMP in the E. coli membrane was assessed using direct stochastic optical reconstruction microscopy (dSTORM), which showed a decrease in membrane clustering with cysteine-rich regions containing two mutations. These results indicate that disulphide bond formation was not disrupted by single mutants located in the cysteine-dense regions and was instead compensated by neighbouring cysteines within the pocket in support of this cysteine-rich pocket hypothesis.<\/jats:p>","DOI":"10.3390\/biology9100344","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T09:28:23Z","timestamp":1603186103000},"page":"344","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Super-Resolution Fluorescence Microscopy Reveals Clustering Behaviour of Chlamydia pneumoniae\u2019s Major Outer Membrane Protein"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0869-0459","authenticated-orcid":false,"given":"Amy E.","family":"Danson","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Reading, Berkshire RG6 6AS, UK"},{"name":"Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, UK"},{"name":"Research Complex at Harwell, Harwell Science and Innovation Campus, Oxfordshire OX11 0FA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1984-9456","authenticated-orcid":false,"given":"Alex","family":"McStea","sequence":"additional","affiliation":[{"name":"Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford OX11 0QX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9141-5217","authenticated-orcid":false,"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford OX11 0QX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Y.","family":"Pollitt","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Reading, Berkshire RG6 6AS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5496-6300","authenticated-orcid":false,"given":"Marisa L.","family":"Martin-Fernandez","sequence":"additional","affiliation":[{"name":"Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford OX11 0QX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7427-5467","authenticated-orcid":false,"given":"Isabel","family":"Moraes","sequence":"additional","affiliation":[{"name":"Research Complex at Harwell, Harwell Science and Innovation Campus, Oxfordshire OX11 0FA, UK"},{"name":"National Physical Laboratory, Teddington TW11 0LW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin A.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, UK"},{"name":"Research Complex at Harwell, Harwell Science and Innovation Campus, Oxfordshire OX11 0FA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila","family":"MacIntyre","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Reading, Berkshire RG6 6AS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9987-8539","authenticated-orcid":false,"given":"Kimberly A.","family":"Watson","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Reading, Berkshire RG6 6AS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1099\/00207713-39-1-88","article-title":"Chlamydia Pneumoniae Sp. 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