{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T20:54:59Z","timestamp":1768424099779,"version":"3.49.0"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013209","name":"Hellenic Foundation for Research and Innovation","doi-asserted-by":"publisher","award":["886"],"award-info":[{"award-number":["886"]}],"id":[{"id":"10.13039\/501100013209","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>3-mercaptopyruvate sulfurtransferase (3-MST) is an enzyme capable of synthesizing hydrogen sulfide (H2S) and polysulfides. In spite of its ubiquitous presence in mammalian cells, very few studies have investigated its contribution to homeostasis and disease development, thus the role of 3-MST remains largely unexplored. Here, we present a clustered, regularly interspaced, short palindromic repeats (CRISPR)\/CRISPR\u2013associated protein-9 (Cas9) induced 3-mst mutant zebrafish line, which will allow the study of 3-MST\u2019s role in several biological processes. The 3-mst zebrafish orthologue was identified using a bioinformatic approach and verified by its ability to produce H2S in the presence of 3-mercaptopyruvate (3-MP). Its expression pattern was analyzed during zebrafish early development, indicating predominantly an expression in the heart and central nervous system. As expected, no detectable levels of 3-Mst protein were observed in homozygous mutant larvae. In line with this, H2S levels were reduced in 3-mst\u2212\/\u2212 zebrafish. Although the mutants showed no obvious morphological deficiencies, they exhibited increased lethality under oxidative stress conditions. The elevated levels of reactive oxygen species, detected following 3-mst deletion, are likely to drive this phenotype. In line with the increased ROS, we observed accelerated fin regenerative capacity in 3-mst deficient zebrafish. Overall, we provide evidence for the expression of 3-mst in zebrafish, confirm its important role in redox homeostasis and indicate the enzyme\u2019s possible involvement in the regeneration processes.<\/jats:p>","DOI":"10.3390\/biom10020317","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T10:49:16Z","timestamp":1582282156000},"page":"317","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Generation and Characterization of a CRISPR\/Cas9\u2014Induced 3-mst Deficient Zebrafish"],"prefix":"10.3390","volume":"10","author":[{"given":"Antonia","family":"Katsouda","sequence":"first","affiliation":[{"name":"Center for Clinical, Experimental Surgery &amp; Translational Research, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece"},{"name":"Laboratory of Pharmacology, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Peleli","sequence":"additional","affiliation":[{"name":"Center for Clinical, Experimental Surgery &amp; Translational Research, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece"},{"name":"Laboratory of Pharmacology, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6933-9625","authenticated-orcid":false,"given":"Antonia","family":"Asimakopoulou","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4253-5930","authenticated-orcid":false,"given":"Andreas","family":"Papapetropoulos","sequence":"additional","affiliation":[{"name":"Center for Clinical, Experimental Surgery &amp; Translational Research, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece"},{"name":"Laboratory of Pharmacology, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2579-7848","authenticated-orcid":false,"given":"Dimitris","family":"Beis","sequence":"additional","affiliation":[{"name":"Center for Clinical, Experimental Surgery &amp; Translational Research, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.bcp.2017.09.010","article-title":"A timeline of hydrogen sulfide (H2S) research: From environmental toxin to biological mediator","volume":"149","author":"Szabo","year":"2018","journal-title":"Biochem. 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