{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:01:52Z","timestamp":1787619712602,"version":"build-2736575974"},"reference-count":78,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:00:00Z","timestamp":1583107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Bone defects cause aesthetic and functional changes that affect the social, economic and especially the emotional life of human beings. This complication stimulates the scientific community to investigate strategies aimed at improving bone reconstruction processes using complementary therapies. Photobiomodulation therapy (PBMT) and the use of new biomaterials, including heterologous fibrin biopolymer (HFB), are included in this challenge. The objective of the present study was to evaluate the influence of photobiomodulation therapy on bone tibial reconstruction of rats with biomaterial consisting of lyophilized bovine bone matrix (BM) associated or not with heterologous fibrin biopolymer. Thirty male rats were randomly separated into three groups of 10 animals. In all animals, after the anesthetic procedure, a noncritical tibial defect of 2 mm was performed. The groups received the following treatments: Group 1: BM + PBMT, Group 2: BM + HFB and Group 3: BM + HFB + PBMT. The animals from Groups 1 and 3 were submitted to PBMT in the immediate postoperative period and every 48 h until the day of euthanasia that occurred at 14 and 42 days. Analyses by computed microtomography (\u00b5CT) and histomorphometry showed statistical difference in the percentage of bone formation between Groups 3 (BM + HB + PBMT) and 2 (BM + HFB) (26.4% \u00b1 1.03% and 20.0% \u00b1 1.87%, respectively) at 14 days and at 42 days (38.2% \u00b1 1.59% and 31.6% \u00b1 1.33%, respectively), and at 42 days there was presence of bone with mature characteristics and organized connective tissue. The \u00b5CT demonstrated BM particles filling the defect and the deposition of new bone in the superficial region, especially in the ruptured cortical. It was concluded that the association of PBMT with HFB and BM has the potential to assist in the process of reconstructing bone defects in the tibia of rats.<\/jats:p>","DOI":"10.3390\/biom10030383","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T07:35:30Z","timestamp":1583134530000},"page":"383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1226-3249","authenticated-orcid":false,"given":"Marcelie Priscila de Oliveira","family":"Rosso","sequence":"first","affiliation":[{"name":"Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Alameda Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75-Vila Universitaria, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Tiemi","family":"Oyadomari","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Alameda Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75-Vila Universitaria, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2858-4717","authenticated-orcid":false,"given":"Karina Torres","family":"Pomini","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Alameda Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75-Vila Universitaria, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0671-6531","authenticated-orcid":false,"given":"Bruna Botteon","family":"Della Coletta","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Alameda Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75-Vila Universitaria, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Vitor Tadashi Cosin","family":"Shindo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Alameda Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75-Vila Universitaria, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui Seabra","family":"Ferreira J\u00fanior","sequence":"additional","affiliation":[{"name":"Center for the Study of Venoms and Venomous Animals (CEVAP), S\u00e3o Paulo State University (Univ. Estadual Paulista, UNESP), Botucatu 18610-307, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedito","family":"Barraviera","sequence":"additional","affiliation":[{"name":"Center for the Study of Venoms and Venomous Animals (CEVAP), S\u00e3o Paulo State University (Univ. Estadual Paulista, UNESP), Botucatu 18610-307, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2564-3761","authenticated-orcid":false,"given":"Claudia Vilalva","family":"Cassaro","sequence":"additional","affiliation":[{"name":"Center for the Study of Venoms and Venomous Animals (CEVAP), S\u00e3o Paulo State University (Univ. Estadual Paulista, UNESP), Botucatu 18610-307, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Vieira","family":"Buchaim","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Avenue Hygino Muzzy Filho, 1001, Mar\u00edlia 17525-902, S\u00e3o Paulo, Brazil"},{"name":"Medical School, University Center of Adamantina (UniFAI), Nove de Julho Street, 730-Centro, Adamantina 17800-000, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8172-7911","authenticated-orcid":false,"given":"Daniel de Bortoli","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Avenue Hygino Muzzy Filho, 1001, Mar\u00edlia 17525-902, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5035-876X","authenticated-orcid":false,"given":"Sandra Maria","family":"Barbalho","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Avenue Hygino Muzzy Filho, 1001, Mar\u00edlia 17525-902, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murilo Priori","family":"Alcalde","sequence":"additional","affiliation":[{"name":"Department of Health Science, University of the Sacred Heart (USC), Bauru 17011-160, S\u00e3o Paulo, Brazil"},{"name":"Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Antonio Hungaro","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus Carlos","family":"Andreo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Alameda Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75-Vila Universitaria, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5881-2218","authenticated-orcid":false,"given":"Rog\u00e9rio Leone","family":"Buchaim","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of S\u00e3o Paulo (USP), Alameda Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75-Vila Universitaria, Bauru 17012-901, S\u00e3o Paulo, Brazil"},{"name":"Postgraduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Avenue Hygino Muzzy Filho, 1001, Mar\u00edlia 17525-902, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s41232-018-0065-x","article-title":"Periodontal Infectogenomics","volume":"38","author":"Kaur","year":"2018","journal-title":"Inflamm. 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