{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:26:31Z","timestamp":1790835991957,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T00:00:00Z","timestamp":1584921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004497","name":"Onderzoeksraad, KU Leuven","doi-asserted-by":"publisher","award":["C12\/16\/023"],"award-info":[{"award-number":["C12\/16\/023"]}],"id":[{"id":"10.13039\/501100004497","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004497","name":"Onderzoeksraad, KU Leuven","doi-asserted-by":"publisher","award":["C14\/19\/095"],"award-info":[{"award-number":["C14\/19\/095"]}],"id":[{"id":"10.13039\/501100004497","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BE 6338\/1-1"],"award-info":[{"award-number":["BE 6338\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004727","name":"VIB","doi-asserted-by":"publisher","award":["not applicable"],"award-info":[{"award-number":["not applicable"]}],"id":[{"id":"10.13039\/501100004727","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fondation Berthe Fouassier\/Fondation de France","award":["not applicable"],"award-info":[{"award-number":["not applicable"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Vascular development is an orchestrated process of vessel formation from pre-existing vessels via sprouting and intussusceptive angiogenesis as well as vascular remodeling to generate the mature vasculature. Bone morphogenetic protein (BMP) signaling via intracellular SMAD1 and SMAD5 effectors regulates sprouting angiogenesis in the early mouse embryo, but its role in other processes of vascular development and in other vascular beds remains incompletely understood. Here, we investigate the function of SMAD1\/5 during early postnatal retinal vascular development using inducible, endothelium-specific deletion of Smad1 and Smad5. We observe the formation of arterial-venous malformations in areas with high blood flow, and fewer and less functional tip cells at the angiogenic front. The vascular plexus region is remarkably hyperdense and this is associated with reduced vessel regression and aberrant vascular loop formation. Taken together, our results highlight important functions of SMAD1\/5 during vessel formation and remodeling in the early postnatal retina.<\/jats:p>","DOI":"10.3390\/biom10030488","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T13:04:04Z","timestamp":1585055044000},"page":"488","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["BMP-SMAD1\/5 Signaling Regulates Retinal Vascular Development"],"prefix":"10.3390","volume":"10","author":[{"given":"Andreas","family":"Benn","sequence":"first","affiliation":[{"name":"Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, 3000 Leuven, Belgium"},{"name":"VIB-KU Leuven Center for Brain &amp; Disease Research, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6794-1513","authenticated-orcid":false,"given":"Florian","family":"Alonso","sequence":"additional","affiliation":[{"name":"Centre de Recherche Cardio-Thoracique de Bordeaux (INSERM U0145), Universit\u00e9 de Bordeaux, 33607 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo","family":"Mangelschots","sequence":"additional","affiliation":[{"name":"Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"G\u00e9not","sequence":"additional","affiliation":[{"name":"Centre de Recherche Cardio-Thoracique de Bordeaux (INSERM U0145), Universit\u00e9 de Bordeaux, 33607 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marleen","family":"Lox","sequence":"additional","affiliation":[{"name":"Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8583-1721","authenticated-orcid":false,"given":"An","family":"Zwijsen","sequence":"additional","affiliation":[{"name":"Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7264","DOI":"10.1038\/ncomms8264","article-title":"Alk1 and Alk5 inhibition by Nrp1 controls vascular sprouting downstream of Notch","volume":"6","author":"Aspalter","year":"2015","journal-title":"Nat. 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