{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:10:23Z","timestamp":1790637023410,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017M3A9D8048414"],"award-info":[{"award-number":["2017M3A9D8048414"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Bovine submaxillary mucin (BSM) is a gel-forming glycoprotein polymer, and Ser\/Thr-linked glycans (O-glycans) are important in regulating BSM\u2019s viscoelasticity and polymerization. However, details of O-glycosylation have not been reported. This study investigates the structural and quantitative characteristics of O-glycans and identifies O-glycosylation sites in BSM using liquid chromatography\u2013tandem mass spectrometry. The O-glycans (consisting of di- to octa-saccharides) and their quantities (%) relative to total O-glycans (100%; 1.1 pmol per 1 \u03bcg of BSM) were identified with 14 major (&gt;1.0%), 12 minor (0.1%\u20131.0%), and eight trace (&lt;0.1%) O-glycans, which were characterized based on their constituents (sialylation (14 O-glycans; 81.9%, sum of relative quantities of each glycan), non-sialylation (20; 18.1%), fucosylation (20; 17.5%), and terminal-galactosylation (6; 3.6%)) and six core structure types [Gal-GalNAc, Gal-(GlcNAc)GalNAc, GlcNAc-GalNAc, GlcNAc-(GlcNAc)GalNAc, and GalNAc-GalNAc]. O-glycosylation sites were identified using O-glycopeptides (bold underlined; 56SGETRTSVI, 259SHSSSGRSRTI, 272GSPSSVSSAEQI, 307RPSYGAL, 625QTLGPL, 728TMTTRTSVVV, and 1080RPEDNTAVA) obtained from proteolytic BSM; these sites are in the four domains of BSM. The gel-forming mucins share common domain structures and glycosylation patterns; these results could provide useful information on mucin-type O-glycans. This is the first study to characterize O-glycans and identify O-glycosylation sites in BSM.<\/jats:p>","DOI":"10.3390\/biom10040636","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T05:48:52Z","timestamp":1587448132000},"page":"636","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Structural and Quantitative Characterization of Mucin-Type O-Glycans and the Identification of O-Glycosylation Sites in Bovine Submaxillary Mucin"],"prefix":"10.3390","volume":"10","author":[{"given":"Jihye","family":"Kim","sequence":"first","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changsoo","family":"Ryu","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jongkwan","family":"Ha","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junmyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghwi","family":"Kim","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minkyoo","family":"Ji","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Park","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaeryong","family":"Lee","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Environmental &amp; Health Chemistry, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha","family":"Kim","sequence":"additional","affiliation":[{"name":"Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1146\/annurev.ph.57.030195.003223","article-title":"Mucin biophysics","volume":"57","author":"Bansil","year":"1995","journal-title":"Annu. 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