{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:51:20Z","timestamp":1791474680049,"version":"4.3.4"},"reference-count":36,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T00:00:00Z","timestamp":1587600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008666","name":"Fundaci\u00f3 la Marat\u00f3 de TV3","doi-asserted-by":"publisher","award":["201536-10"],"award-info":[{"award-number":["201536-10"]}],"id":[{"id":"10.13039\/100008666","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012513","name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red Enfermedades Cardiovasculares","doi-asserted-by":"publisher","award":["CIBERCV"],"award-info":[{"award-number":["CIBERCV"]}],"id":[{"id":"10.13039\/501100012513","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Previous studies demonstrated a reduction in myocardial scar size in heterozygous Cx43+\/- mice subjected to permanent coronary occlusion. However, patients presenting with ST segment elevation myocardial infarction often undergo rapid coronary revascularization leading to prompt restoration of coronary flow. Therefore, we aimed to assess changes in scar size and left ventricular remodeling following transient myocardial ischemia (45 min) followed by 14 days of reperfusion using Cx43fl\/fl (controls) and Cx43Cre-ER(T)\/fl inducible knock-out (Cx43 content: 50%) mice treated with vehicle or 4-hydroxytamoxifen (4-OHT) to induce a Cre-ER(T)-mediated global deletion of the Cx43 floxed allele. The scar area (picrosirius red), measured 14 days after transient coronary occlusion, was similarly reduced in both vehicle and 4-OHT-treated Cx43Cre-ER(T)\/fl mice, compared to Cx43fl\/fl animals, having normal Cx43 levels (15.78% \u00b1 3.42% and 16.54% \u00b1 2.31% vs. 25.40% \u00b1 3.14% and 22.43% \u00b1 3.88% in vehicle and 4-OHT-treated mice, respectively, p = 0.027). Left ventricular dilatation was significantly attenuated in both Cx43-deficient groups (p = 0.037 for left ventricular end-diastolic diameter). These protective effects were correlated with an attenuated enhancement in pro-transforming growth factor beta 1 (TGF\u03b21) expression after reperfusion. In conclusion, our data demonstrate that Cx43 deficiency induces a protective effect on scar formation after transient coronary occlusion in mice, an effect associated with reduced left ventricular remodeling and attenuated enhancement in pro-TGF\u03b21 expression.<\/jats:p>","DOI":"10.3390\/biom10040651","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T10:46:22Z","timestamp":1587638782000},"page":"651","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Connexin 43 Deficiency Is Associated with Reduced Myocardial Scar Size and Attenuated TGF\u03b21 Signaling after Transient Coronary Occlusion in Conditional Knock-Out Mice"],"prefix":"10.3390","volume":"10","author":[{"given":"Laura","family":"Valls-Lacalle","sequence":"first","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Consegal","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisol","family":"Ruiz-Meana","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bego\u00f1a","family":"Benito","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4736-5010","authenticated-orcid":false,"given":"Javier","family":"Inserte","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignasi","family":"Barba","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Ferreira-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2930-8773","authenticated-orcid":false,"given":"Antonio","family":"Rodr\u00edguez-Sinovas","sequence":"additional","affiliation":[{"name":"Cardiovascular Diseases Research Group, Department of Cardiology, Vall d\u2019Hebron University Hospital and Research Institute, Departament de Medicina, Universitat Aut\u00f2noma de Barcelona, Pg. Vall d\u2019Hebron 119-129, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s00441-014-2020-8","article-title":"Connexins in the heart","volume":"360","author":"Lambiase","year":"2014","journal-title":"Cell Tissue Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.bbamem.2005.04.001","article-title":"Structural organization of gap junction channels","volume":"1711","author":"Sosinsky","year":"2005","journal-title":"Biochimica et Biophysica Acta (BBA) Biomembranes"},{"key":"ref_3","first-page":"404","article-title":"Role of the intercalated disc in cardiac propagation and arrhythmogenesis","volume":"5","author":"Saffitz","year":"2014","journal-title":"Front. 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