{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:15:58Z","timestamp":1788347758257,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1A2B4004618, 2017R1A5A2015391"],"award-info":[{"award-number":["2017R1A2B4004618, 2017R1A5A2015391"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The precise regulation of osteoclast differentiation and function is crucial for the maintenance of healthy bone. Despite several reports of collagenase expression in bone tissues, the precise isoform expression as well as the role in osteoclasts are still unclear. In the present report, the expression of matrix metalloprotease (MMP)8 and MMP13 was confirmed in mouse bone marrow macrophage osteoclast precursors. The mRNA and protein expressions of both collagenases were significantly reduced by receptor activator of nuclear factor \u03baB ligand (RANKL) stimulation. Notably, either inhibition of MMP expression by siRNA or treatment of cells with collagenase inhibitor Ro 32-3555 significantly augmented osteoclast fusion and resorption activity without affecting the osteoclast number. The inhibition of collagenase by Ro 32-3555 increased the expression of osteoclast fusion genes, Atp6v0d2 and Dcstamp, without affecting nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) protein expression. The enhanced osteoclast fusion by collagenase inhibition appears to be mediated through an extracellular signal regulated kinase (ERK)-dependent pathway. Collectively, these data provide novel information on the regulation of osteoclast fusion process.<\/jats:p>","DOI":"10.3390\/biom10050705","type":"journal-article","created":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T06:41:20Z","timestamp":1588660880000},"page":"705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Endogenous Collagenases Regulate Osteoclast Fusion"],"prefix":"10.3390","volume":"10","author":[{"given":"Hyo Jeong","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9049-6908","authenticated-orcid":false,"given":"Youngkyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, Korea"},{"name":"Institute for Hard Tissue and Bio-tooth Regeneration (IHBR), School of Dentistry, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/nature01658","article-title":"Osteoclast differentiation and activation","volume":"423","author":"Boyle","year":"2003","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1126\/science.289.5484.1504","article-title":"Bone resorption by osteoclasts","volume":"289","author":"Teitelbaum","year":"2000","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1126\/science.289.5484.1508","article-title":"Therapeutic approaches to bone diseases","volume":"289","author":"Rodan","year":"2000","journal-title":"Science"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1002\/1529-0131(200011)43:11<2523::AID-ANR20>3.0.CO;2-Z","article-title":"Expression of osteoclast differentiation factor in rheumatoid arthritis","volume":"43","author":"Shigeyama","year":"2000","journal-title":"Arthritis Rheumatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.1600-0757.2010.00347.x","article-title":"Mechanisms and control of pathologic bone loss in periodontitis","volume":"53","author":"Bartold","year":"2010","journal-title":"Periodontol 2000"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0006-291X(03)00695-8","article-title":"Signal transduction by receptor activator of nuclear factor kB in osteoclasts","volume":"305","author":"Lee","year":"2003","journal-title":"Biochem. 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