{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T12:26:45Z","timestamp":1780576005486,"version":"3.54.1"},"reference-count":49,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T00:00:00Z","timestamp":1589846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009647","name":"ministry of health","doi-asserted-by":"publisher","award":["C75E17000050001"],"award-info":[{"award-number":["C75E17000050001"]}],"id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The protein MucR from Brucella abortus has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros\/MucR family comprising proteins that control the expression of genes important for the successful interaction of \u03b1-proteobacteria with their eukaryotic hosts. Despite clear evidence of the role of MucR in repressing virulence genes, no study has been carried out so far demonstrating the direct interaction of this protein with the promoter of its target gene babR encoding a LuxR-like regulator repressing virB genes. In this study, we show for the first time the ability of MucR to bind the promoter of babR in electrophoretic mobility shift assays demonstrating a direct role of MucR in repressing this gene. Furthermore, we demonstrate that MucR can bind the virB gene promoter. Analyses by RT-qPCR showed no significant differences in the expression level of virB genes in Brucella abortus CC092 lacking MucR compared to the wild-type Brucella abortus strain, indicating that MucR binding to the virB promoter has little impact on virB gene expression in B. abortus 2308. The MucR modality to bind the two promoters analyzed supports our previous hypothesis that this is a histone-like protein never found before in Brucella.<\/jats:p>","DOI":"10.3390\/biom10050788","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T02:48:24Z","timestamp":1589942904000},"page":"788","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Different Impacts of MucR Binding to the babR and virB Promoters on Gene Expression in Brucella abortus 2308"],"prefix":"10.3390","volume":"10","author":[{"given":"Giorgia","family":"Borriello","sequence":"first","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d, via Vivaldi\u201443, 81100 Caserta, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubina","family":"Paradiso","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marita Georgia","family":"Riccardi","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Criscuolo","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4768-0165","authenticated-orcid":false,"given":"Gaetano","family":"Verde","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Biophysics (IGB) \u201cAdriano Buzzati-Traverso\u201d, Consiglio Nazionale delle Ricerche (CNR), 80134 Naples, Italy"},{"name":"Flomics Biotech, Carrer Dr. Aiguader 88, 08003 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosangela","family":"Marasco","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d, via Vivaldi\u201443, 81100 Caserta, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo Vincenzo","family":"Pedone","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d, via Vivaldi\u201443, 81100 Caserta, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Galiero","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Baglivo","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d, via Vivaldi\u201443, 81100 Caserta, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1056\/NEJMra050570","article-title":"Brucellosis","volume":"352","author":"Pappas","year":"2005","journal-title":"N. 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