{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T12:03:44Z","timestamp":1779365024605,"version":"3.53.0"},"reference-count":96,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T00:00:00Z","timestamp":1595203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Prion diseases are fatal, transmissible neurodegenerative disorders whose pathogenesis is driven by the misfolding, self-templating and cell-to-cell spread of the prion protein. Other neurodegenerative diseases such as Alzheimer\u2019s disease, Parkinson\u2019s disease, amyotrophic lateral sclerosis and Huntington\u2019s disease, share some of these prion-like features, with different aggregation-prone proteins. Consequently, researchers have begun to apply prion-specific techniques, like the prion organotypic slice culture assay (POSCA), to these disorders. In this review we explore the ways in which the prion phenomenon has been used in organotypic cultures to study neurodegenerative diseases from the perspective of protein aggregation and spreading, strain propagation, the role of glia in pathogenesis, and efficacy of drug treatments. We also present an overview of the advantages and disadvantages of this culture system compared to in vivo and in vitro models and provide suggestions for new directions.<\/jats:p>","DOI":"10.3390\/biom10071079","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T10:59:38Z","timestamp":1595242778000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["POSCAbilities: The Application of the Prion Organotypic Slice Culture Assay to Neurodegenerative Disease Research"],"prefix":"10.3390","volume":"10","author":[{"given":"Hailey","family":"Pineau","sequence":"first","affiliation":[{"name":"Centre for Prions and Protein Folding Diseases, University of Alberta, 204 Brain and Aging Research Building, 8710-112 St, Edmonton, AB T6G 2M8, Canada"},{"name":"Neuroscience and Mental Health Institute, University of Alberta, 2-132 Li Ka Shing, Edmonton, AB T6G 2E1, Canada"},{"name":"Division of Neurology, Department of Medicine, Faculty of Medicine &amp; Dentistry, University of Alberta, Clinical Sciences Building, 8440-112 St, Edmonton, AB T6G 2B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-8666","authenticated-orcid":false,"given":"Valerie","family":"Sim","sequence":"additional","affiliation":[{"name":"Centre for Prions and Protein Folding Diseases, University of Alberta, 204 Brain and Aging Research Building, 8710-112 St, Edmonton, AB T6G 2M8, Canada"},{"name":"Neuroscience and Mental Health Institute, University of Alberta, 2-132 Li Ka Shing, Edmonton, AB T6G 2E1, Canada"},{"name":"Division of Neurology, Department of Medicine, Faculty of Medicine &amp; Dentistry, University of Alberta, Clinical Sciences Building, 8440-112 St, Edmonton, AB T6G 2B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1126\/science.6801762","article-title":"Novel proteinaceous infectious particles cause scrapie","volume":"216","author":"Prusiner","year":"1982","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0065-3527(08)60038-X","article-title":"Molecular biology and genetics of neurodegenerative diseases caused by prions","volume":"41","author":"Prusiner","year":"1992","journal-title":"Adv. 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