{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T16:49:25Z","timestamp":1789404565847,"version":"build-2803163510"},"reference-count":49,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T00:00:00Z","timestamp":1599004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673317, 81602979, 81530091"],"award-info":[{"award-number":["81673317, 81602979, 81530091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018621","name":"Program for Changjiang Scholars and Innovative Research Team in University","doi-asserted-by":"publisher","award":["IRT_17R68"],"award-info":[{"award-number":["IRT_17R68"]}],"id":[{"id":"10.13039\/501100018621","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Open Funding Project of State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University","award":["MMLKF17-09"],"award-info":[{"award-number":["MMLKF17-09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Proansamycin X, a hypothetical earliest macrocyclic precursor in the biosynthesis of rifamycin, had never been isolated and identified. According to bioinformatics analysis, it was proposed that RifT (a putative NADH-dependent dehydrogenase) may be a candidate target responsible for the dehydrogenation of proansamycin X. In this study, the mutant strain Amycolatopsis mediterranei S699 \u0394rifT was constructed by deleting the rifT gene. From this strain, eleven 8-deoxy-rifamycin derivatives (1\u201311) and seven known analogues (12\u201318) were isolated. Their structures were elucidated by extensive analysis of 1D and 2D NMR spectroscopic data and high-resolution ESI mass spectra. Compound 1 is a novel amide N-glycoside of seco-rifamycin. Compounds 2 and 3 feature conserved 11,12-seco-rifamycin W skeleton. The diverse post-modifications in the polyketide chain led to the production of 4\u201311. Compounds 2, 3, 5, 6, 13 and 15 exhibited antibacterial activity against Staphylococcus aureus (MIC (minimal inhibitory concentration) values of 10, 20, 20, 20, 40 and 20 \u03bcg\/mL, respectively). Compounds 14, 15, 16, 17 and 18 showed potent antiproliferative activity against KG1 cells with IC50 (half maximal inhibitory concentration) values of 14.91, 44.78, 2.16, 18.67 and 8.07 \u03bcM, respectively.<\/jats:p>","DOI":"10.3390\/biom10091265","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T09:29:28Z","timestamp":1599038968000},"page":"1265","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["8-Deoxy-Rifamycin Derivatives from Amycolatopsis mediterranei S699 \u0394rifT Strain"],"prefix":"10.3390","volume":"10","author":[{"given":"Feng","family":"Ye","sequence":"first","affiliation":[{"name":"Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3738-2060","authenticated-orcid":false,"given":"Yanrong","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengliang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiying","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoxin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3881-0135","authenticated-orcid":false,"given":"Yuemao","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan 250012, China"},{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"ref_1","first-page":"231","article-title":"Chemistry of the ansamycin antibiotics","volume":"Volume 33","author":"Rinehart","year":"1976","journal-title":"Fortschritte der Chemie Organischer Naturstoffe"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/BFb0048448","article-title":"Ansamycins chemistry, biosynthesis and biological activity","volume":"Volume 72","author":"Wehrli","year":"1977","journal-title":"Topics in Current Chemistry"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1159\/000220462","article-title":"Rifampicin: A new orally active rifamycin","volume":"11","author":"Maggi","year":"1966","journal-title":"Chemotherapy"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1248\/cpb.52.1","article-title":"Recent developments in the maytansinoid antitumor agents","volume":"52","author":"Cassady","year":"2004","journal-title":"Chem. 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