{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T05:56:24Z","timestamp":1774504584426,"version":"3.50.1"},"reference-count":20,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,5]],"date-time":"2020-09-05T00:00:00Z","timestamp":1599264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovak Research and Development Agency, Ministry of Education, Science, Research and Sport of the Slovak Republic","award":["APVV-17-0017"],"award-info":[{"award-number":["APVV-17-0017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The dynamic development of venomics in recent years has resulted in a significant increase in publicly available proteomic data. The information contained therein is often used for comparisons between different datasets and to draw biological conclusions therefrom. In this article, we aimed to show the possible differences that can arise, in the final results of the proteomic experiment, while using different research workflows. We applied two software solutions (PeptideShaker and MaxQuant) to process data from shotgun LC-MS\/MS analysis of Naja ashei venom and collate it with the previous report concerning this species. We were able to provide new information regarding the protein composition of this venom but also present the qualitative and quantitative limitations of currently used proteomic methods. Moreover, we reported a rapid and straightforward technique for the separation of the fraction of proteins from the three-finger toxin family. Our results underline the necessary caution in the interpretation of data based on a comparative analysis of data derived from different studies.<\/jats:p>","DOI":"10.3390\/biom10091282","type":"journal-article","created":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T23:12:49Z","timestamp":1599433969000},"page":"1282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Different Research Approaches in Unraveling the Venom Proteome of Naja ashei"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2723-3691","authenticated-orcid":false,"given":"Konrad Kamil","family":"Hus","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0141ukasz","family":"Marczak","sequence":"additional","affiliation":[{"name":"Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12\/14, 61-704 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladim\u00edr","family":"Petrilla","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovakia"},{"name":"Zoological Department, Zoological Garden Ko\u0161ice, \u0160irok\u00e1 31, 040 06 Ko\u0161ice-Kave\u010dany, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Petrillov\u00e1","sequence":"additional","affiliation":[{"name":"Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Kosice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslav","family":"Leg\u00e1th","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"},{"name":"Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1907-9399","authenticated-orcid":false,"given":"Aleksandra","family":"Bocian","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powsta\u0144c\u00f3w Warszawy 6, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1080\/14789450.2018.1500904","article-title":"Snake venomics\u2014From low-resolution toxin-pattern recognition to toxin-resolved venom proteomes with absolute quantification","volume":"15","author":"Calvete","year":"2018","journal-title":"Expert Rev. 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