{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T04:04:58Z","timestamp":1786421098351,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"King Saud University, Riyadh, Saudi Arabia","award":["RSP\/2020\/45"],"award-info":[{"award-number":["RSP\/2020\/45"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Detoxification is one of the main vital tasks performed by the liver. The purpose of this study was to investigate whether mustard in its normal or nanoparticles could confer a protective\/therapeutic effect against TAA-induced acute liver failure in experimental animal models. Mustard ethanolic extract was analyzed by HPLC\/MS. To induce liver failure, male rats were injected with 350 mg\/kg bw TAA IP, then treated orally with a dose of 100 mg\/kg for 15 d of mustard extract and its nanoform before and following induction. The levels of serum liver functions, total cholesterol (TCHo), total glyceride (TG), total bilirubin (TBIL), hepatic malonaldhyde (MDA) and nitric oxide (NO),glutathione (GSH), sodium oxide dismutase (SOD), as well as tumor necrosis factor (TNF-\u03b1,) and interleukin 6 (IL-6), were estimated. DNA genotoxicity and hepatic pathology, and immunohistologic (IHC) changes were assayed. The antioxidant content of Phenolic acids, flavonoids in mustard ethanolic extract substantially decreased the levels of ALT, AST, ALP and rehabilitated the histopathological alterations. In addition, nanoforms of mustard ethanol extract have notably increased the levels of GSH, SOD and significantly reduced the levels of MDA. The expression levels of TNF-\u03b1 and IL-6 in serum and tissue were markedly downregulated. DNA genotoxicity was significantly reversed. Mustard introduced a protective and medicinal effect against TAA in both its forms.<\/jats:p>","DOI":"10.3390\/biom10121650","type":"journal-article","created":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T09:17:58Z","timestamp":1607505478000},"page":"1650","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Brassica juncea L. (Mustard) Extract Silver NanoParticles and Knocking off Oxidative Stress, ProInflammatory Cytokine and Reverse DNA Genotoxicity"],"prefix":"10.3390","volume":"10","author":[{"given":"Sohair Aly","family":"Hassan","sequence":"first","affiliation":[{"name":"Therapeutic Chemistry Department, Pharmaceutical Industries Division, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Mohamed El","family":"Hagrassi","sequence":"additional","affiliation":[{"name":"Phytochemistry and Plant Systematics Department, Pharmaceutical Industries Division, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4965-5804","authenticated-orcid":false,"given":"Olfat","family":"Hammam","sequence":"additional","affiliation":[{"name":"Pathology Department, Theodor Bilharz Research Institute, P.O. Box 30, El Warraq, Giza Governorate 12411, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8816-5309","authenticated-orcid":false,"given":"Abdelmohsen M.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Therapeutic Chemistry Department, Pharmaceutical Industries Division, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6083-5913","authenticated-orcid":false,"given":"Essam","family":"Ezzeldin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3655-8490","authenticated-orcid":false,"given":"Wessam Magdi","family":"Aziz","sequence":"additional","affiliation":[{"name":"Therapeutic Chemistry Department, Pharmaceutical Industries Division, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s40264-018-0743-2","article-title":"Drug-induced liver injury: Highlights of the recent literature","volume":"42","author":"Real","year":"2019","journal-title":"Drug Saf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1007\/s00210-018-1540-2","article-title":"Glibenclamide protects against thioacetamide-induced hepatic damage i Wistar rat: Investigation on NLRP3, MMP-2, and stellate cell activation","volume":"391","author":"Dwivedi","year":"2018","journal-title":"Naunyn Schmiedeberg Arch. 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