{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T16:09:03Z","timestamp":1789834143764,"version":"4.0.1"},"reference-count":101,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:00:00Z","timestamp":1614643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wilhelm Doerenkamp-stiftung with the Natvantage grant","award":["No 1027085801"],"award-info":[{"award-number":["No 1027085801"]}]},{"name":"Federal Ministry for Economic Affairs and Energy for the central innovation program","award":["Psoritop"],"award-info":[{"award-number":["Psoritop"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation of keratinocytes and expression of pro-inflammatory cytokines in the epidermis. New biological drugs were developed for the systemic treatment of moderate to severe psoriasis. However, products for the topical treatment of mild psoriasis are still required. Here, we examined the effect of natural compounds on psoriasis-like keratinocytes in vitro and ex vivo. Psoriasis-like keratinocytes were generated by treating human primary keratinocytes with the psoriasis-associated cytokines IL-17A, TNF-\u03b1 and IL-22. Initially, 10 botanical extracts from Ayurvedic Medicine, Traditional Chinese Medicine, Northern American traditional medicine and Occidental Monastic Medicine were investigated using BrdU assays and IL-6 and IL-8 ELISAs. Curcuma amada, Humulus lupulus and Hypericum perforatum turned out to be the most effective plant extracts. In vitro, the plant extracts inhibited the expression of anti-microbial peptides (\u03b2-defensin 2), the hyperproliferation marker keratin 17, the glucose transporter 1 and downregulated the nuclear translocation of NF-\u03baB and pSTAT3. In an ex vivo psoriasis model, Humulus lupulus displayed the most prominent anti-proliferative and anti-inflammatory effect. In conclusion, among the plant extracts investigated, Humulus lupulus showed the most promising anti-psoriatic effect. It is an interesting candidate for topical psoriasis treatment that should be further studied in clinical trials.<\/jats:p>","DOI":"10.3390\/biom11030371","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T05:17:16Z","timestamp":1614662236000},"page":"371","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["The Effect of Herbal Medicinal Products on Psoriasis-Like Keratinocytes"],"prefix":"10.3390","volume":"11","author":[{"given":"Fabian","family":"Gendrisch","sequence":"first","affiliation":[{"name":"Research Center skinitial, Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Haarhaus","sequence":"additional","affiliation":[{"name":"Research Center skinitial, Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Krieger","sequence":"additional","affiliation":[{"name":"Research Center skinitial, Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl-Werner","family":"Quirin","sequence":"additional","affiliation":[{"name":"Flavex Naturextrakte GmbH, 66780 Rehlingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph M.","family":"Schempp","sequence":"additional","affiliation":[{"name":"Research Center skinitial, Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0093-560X","authenticated-orcid":false,"given":"Ute","family":"W\u00f6lfle","sequence":"additional","affiliation":[{"name":"Research Center skinitial, Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1038\/nrd2996","article-title":"Psoriasis market","volume":"8","author":"Melnikova","year":"2009","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s00281-019-00766-z","article-title":"Resolution of plaque-type psoriasis: What is left behind (and reinitiates the disease)","volume":"41","author":"Benezeder","year":"2019","journal-title":"Semin. Immunopathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/j.1365-2133.2007.07999.x","article-title":"On the role of the epidermal differentiation complex in ichthyosis vulgaris, atopic dermatitis and psoriasis","volume":"157","author":"Hoffjan","year":"2007","journal-title":"Br. J. Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2168","DOI":"10.1016\/j.jid.2017.05.015","article-title":"Nrf2 Promotes Keratinocyte Proliferation in Psoriasis through Up-Regulation of Keratin 6, Keratin 16, and Keratin 17","volume":"137","author":"Yang","year":"2017","journal-title":"J. Investig. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-019-13756-4","article-title":"Neutrophil extracellular trap-associated RNA and LL37 enable self-amplifying inflammation in psoriasis","volume":"11","author":"Herster","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2340\/00015555-2808","article-title":"Highlighting Interleukin-36 Signalling in Plaque Psoriasis and Pustular Psoriasis","volume":"98","author":"Furue","year":"2018","journal-title":"Acta Derm. Venereol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.it.2012.11.005","article-title":"The IL-23\/T17 pathogenic axis in psoriasis is amplified by keratinocyte responses","volume":"34","author":"Lowes","year":"2013","journal-title":"Trends Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1684\/ejd.2020.3770","article-title":"Anti-psoriatic properties of paeoniflorin: Suppression of the NF-kappaB pathway and Keratin 17","volume":"30","author":"Bai","year":"2020","journal-title":"Eur. J. Dermatol. EJD"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1078\/0944-7113-00032","article-title":"In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins","volume":"8","author":"Sampson","year":"2001","journal-title":"Phytomedicine"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1055\/s-2008-1074533","article-title":"Madecassoside Isolated fromCentella asiaticaHerbs Facilitates Burn Wound Healing in Mice","volume":"74","author":"Liu","year":"2008","journal-title":"Planta Medica"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.biopha.2018.02.115","article-title":"Centella asiatica ameliorates diabetes-induced stress in rat tissues via influences on antioxidants and inflammatory cytokines","volume":"101","author":"Masola","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1002\/ptr.5110","article-title":"Centella asiaticain Dermatology: An Overview","volume":"28","author":"Bylka","year":"2014","journal-title":"Phytother. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Vishnupriya, P., and Padma, V.V. (2017). A Review on the Antioxidant and Therapeutic Potential of Bacopa monnieri. React. Oxyg. Species, 3.","DOI":"10.20455\/ros.2017.817"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/615384","article-title":"Bacopa monnierias an Antioxidant Therapy to Reduce Oxidative Stress in the Aging Brain","volume":"2015","author":"Simpson","year":"2015","journal-title":"Evidence-Based Complement. Altern. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s10616-018-0274-9","article-title":"Induction of apoptosis by in vitro and in vivo plant extracts derived from Menyanthes trifoliata L. in human cancer cells","volume":"71","author":"Kowalczyk","year":"2019","journal-title":"Cytotechnology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2019\/9165784","article-title":"An Evaluation of the DNA-Protective Effects of Extracts from Menyanthes trifoliata L. Plants Derived from In Vitro Culture Associated with Redox Balance and Other Biological Activities","volume":"2019","author":"Kowalczyk","year":"2019","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1080\/10286020.2018.1460363","article-title":"Anti-inflammatory lupane triterpenoids from Menyanthes trifoliata","volume":"21","author":"Zhu","year":"2018","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"105840","DOI":"10.1016\/j.intimp.2019.105840","article-title":"Therapeutic effects of gentiopicroside on adjuvant-induced arthritis by inhibiting inflammation and oxidative stress in rats","volume":"76","author":"Xie","year":"2019","journal-title":"Int. Immunopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gendrisch, F., Nov\u00e1\u010dkov\u00e1, A., Sochorov\u00e1, M., Haarhaus, B., V\u00e1vrov\u00e1, K., Schempp, C.M., and W\u00f6lfle, U. (2020). Gentiana lutea Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes. Molecules, 25.","DOI":"10.3390\/molecules25081832"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"104559","DOI":"10.1016\/j.phrs.2019.104559","article-title":"Gentiopicroside activates the bile acid receptor Gpbar1 (TGR5) to repress NF-kappaB pathway and ameliorate diabetic nephropathy","volume":"151","author":"Xiao","year":"2020","journal-title":"Pharmacol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"731","DOI":"10.21037\/atm.2019.11.126","article-title":"Gentiopicroside (GENT) protects against sepsis induced by lipopolysaccharide (LPS) through the NF-\u03baB signaling pathway","volume":"7","author":"Wang","year":"2019","journal-title":"Ann. Transl. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s12986-017-0180-8","article-title":"Potential therapeutic targets of Guggulsterone in cancer","volume":"14","author":"Bhat","year":"2017","journal-title":"Nutr. Metab."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"294","DOI":"10.2174\/1381612822666151112153117","article-title":"Guggulsterone for Chemoprevention of Cancer","volume":"22","author":"Shishodia","year":"2015","journal-title":"Curr. Pharm. Des."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"686","DOI":"10.3389\/fphar.2018.00686","article-title":"Googling the Guggul (Commiphora and Boswellia) for Prevention of Chronic Diseases","volume":"9","author":"Kunnumakkara","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_25","first-page":"248","article-title":"Hypolipidemic Effects of a Proprietary Commiphora Mukul Gum Resin Extract and Medium-Chain Triglyceride Preparation (GU-MCT810)","volume":"18","author":"Ramachandran","year":"2013","journal-title":"J. Evid. Based Integr. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/jdv.12204","article-title":"Beer and beer compounds: Physiological effects on skin health","volume":"28","author":"Chen","year":"2013","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1021\/np800740m","article-title":"Hop (Humulus lupulus)-Derived Bitter Acids as Multipotent Bioactive Compounds","volume":"72","author":"Cattoor","year":"2009","journal-title":"J. Nat. Prod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1089\/ars.2005.7.1728","article-title":"Evaluation for Safety of Antioxidant Chemopreventive Agents","volume":"7","author":"Kawanishi","year":"2005","journal-title":"Antioxid. Redox Signal."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Weber, N., Biehler, K., Schwabe, K., Haarhaus, B., Quirin, K.-W., Frank, U., Schempp, C.M., and W\u00f6lfle, U. (2019). Hop Extract Acts as an Antioxidant with Antimicrobial Effects against Propionibacterium acnes and Staphylococcus aureus. Molecules, 24.","DOI":"10.3390\/molecules24020223"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1055\/s-0033-1351019","article-title":"Topical Application of St. John\u2019s Wort (Hypericum perforatum)","volume":"80","author":"Seelinger","year":"2013","journal-title":"Planta Med."},{"key":"ref_31","unstructured":"Sevastre-Berghian, A.C., Toma, V.A., Sevastre, B., Hanganu, D., Vlase, L., Benedec, D., Oniga, I., Baldea, I., Olteanu, D., and Moldovan, R. (2019). Characterization and biological effects of Hypericum extracts on experimentally-induced\u2014Anxiety, oxidative stress and inflammation in rats. J. Physiol. Pharmacol. Off. J. Pol. Physiol. Soc., 69."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"You, M.-K., Kim, H.-J., Kook, J.H., and Kim, H.-A. (2018). St. John\u2019s Wort Regulates Proliferation and Apoptosis in MCF-7 Human Breast Cancer Cells by Inhibiting AMPK\/mTOR and Activating the Mitochondrial Pathway. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19040966"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"33942","DOI":"10.1074\/jbc.M801844200","article-title":"Specific TRPC6 Channel Activation, a Novel Approach to Stimulate Keratinocyte Differentiation","volume":"283","author":"Essin","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Leuner, K., Kraus, M., Woelfle, U., Beschmann, H., Harteneck, C., Boehncke, W.-H., Schempp, C.M., and M\u00fcller, W.E. (2011). Reduced TRPC Channel Expression in Psoriatic Keratinocytes Is Associated with Impaired Differentiation and Enhanced Proliferation. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0014716"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1515\/jcim-2015-0005","article-title":"Inhibition of AKT signaling by supercritical CO2 extract of mango ginger (Curcuma amada Roxb.) in human glioblastoma cells","volume":"12","author":"Ramachandran","year":"2015","journal-title":"J. Complement. Integr. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.fitote.2017.08.006","article-title":"Labdane diterpenoids from Curcuma amada rhizomes collected in Myanmar and their antiproliferative activities","volume":"122","author":"Win","year":"2017","journal-title":"Fitoterapia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1002\/ptr.2137","article-title":"Phytochemical, pharmacological and ethnobotanical studies in mango ginger (Curcuma amada Roxb.; Zingiberaceae)","volume":"21","author":"Jatoi","year":"2007","journal-title":"Phytotherapy Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1002\/ptr.5360","article-title":"Therapeutic Effect of Supercritical CO2 Extracts of Curcuma Species with Cancer Drugs in Rhabdomyosarcoma Cell Lines","volume":"29","author":"Ramachandran","year":"2015","journal-title":"Phytotherapy Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"14192","DOI":"10.1074\/jbc.M601074200","article-title":"Alkylamides from Echinacea Are a New Class of Cannabinomimetics","volume":"281","author":"Raduner","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1080\/13880209.2016.1265989","article-title":"Studies on phytochemical, antioxidant, anti-inflammatory, hypoglycaemic and antiproliferative activities of Echinacea purpurea and Echinacea angustifolia extracts","volume":"55","author":"Aarland","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.dci.2017.03.002","article-title":"Echinacea pupurea extracts promote murine dendritic cell maturation by activation of JNK, p38 MAPK and NF-\u03baB pathways","volume":"73","author":"Li","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"ref_42","unstructured":"Nath, S., and Pathak, B. (2021, January 11). Phytochemical and Pharmacological Characteristics of Wrightiatinctoria: A Review. Available online: \/paper\/Phytochemical-and-Pharmacological-Characteristics-%3A-Nath-Pathak\/9f0ce9270365efb25ed698eeeff06c4e920ee67a."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"36","DOI":"10.4103\/0973-7847.125528","article-title":"A review on phytochemical, pharmacological, and pharmacognostical profile of Wrightia tinctoria: Adulterant of kurchi","volume":"8","author":"Srivastava","year":"2014","journal-title":"Pharmacogn. Rev."},{"key":"ref_44","first-page":"1","article-title":"Pharmacological Evaluation of Wrightia Tinctoria\u2014A Review","volume":"5","author":"Babu","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_45","unstructured":"Rao, B., Rajeswari, D., Devarakonda, R., and Battu, H. (2019). Phytochemical and Pharmacological Studies on Wrightia Tinctoria. World J. Pharm. Pharm. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e449","DOI":"10.1111\/j.1365-4632.2012.05611.x","article-title":"Anthralin\/dithranol in dermatology","volume":"53","author":"Sehgal","year":"2014","journal-title":"Int. J. Dermatol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1038\/nri2423","article-title":"New regulators of NF-\u03baB in inflammation","volume":"8","author":"Ghosh","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.foodchem.2011.08.054","article-title":"Determination of anti-tubercular agent in mango ginger (Curcuma amada Roxb.) by reverse phase HPLC-PDA-MS","volume":"131","author":"Singh","year":"2012","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0092-8674(75)80001-8","article-title":"Seria cultivation of strains of human epidemal keratinocytes: The formation keratinizin colonies from single cell is","volume":"6","author":"Rheinwatd","year":"1975","journal-title":"Cell"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-15892-7","article-title":"The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function","volume":"7","author":"Pfaff","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1093\/intimm\/dxw011","article-title":"IL-17A plays a central role in the expression of psoriasis signature genes through the induction of I\u03baB-\u03b6 in keratinocytes","volume":"28","author":"Muromoto","year":"2016","journal-title":"Int. Immunol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1046\/j.1523-1747.1998.00347.x","article-title":"Interleukin-17 and Interferon-\u03b3 Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes","volume":"111","author":"Teunissen","year":"1998","journal-title":"J. Investig. Dermatol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s00109-009-0457-0","article-title":"IL-22 and IL-20 are key mediators of the epidermal alterations in psoriasis while IL-17 and IFN-\u03b3 are not","volume":"87","author":"Wolk","year":"2009","journal-title":"J. Mol. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1365-2249.1994.tb06244.x","article-title":"Elevated tumour necrosis factor-alpha (TNF-\u03b1) biological activity in psoriatic skin lesions","volume":"96","author":"Ettehadi","year":"2008","journal-title":"Clin. Exp. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1111\/j.0022-202X.2005.23935.x","article-title":"High Expression Levels of Keratinocyte Antimicrobial Proteins in Psoriasis Compared with Atopic Dermatitis","volume":"125","author":"Zeeuwen","year":"2005","journal-title":"J. Investig. Dermatol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/j.jaci.2016.06.038","article-title":"\u03b2-Defensin 2 is a responsive biomarker of IL-17A\u2013driven skin pathology in patients with psoriasis","volume":"139","author":"Kolbinger","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Zanet, J., Freije, A., Ruiz, M., Coulon, V., Sanz, J.R., Chiesa, J., and Gandarillas, A. (2010). A Mitosis Block Links Active Cell Cycle with Human Epidermal Differentiation and Results in Endoreplication. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0015701"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/s41591-018-0003-0","article-title":"Differential glucose requirement in skin homeostasis and injury identifies a therapeutic target for psoriasis","volume":"24","author":"Zhang","year":"2018","journal-title":"Nat. Med."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1111\/ijd.14037","article-title":"Glucose transporter-1 (GLUT-1) expression in psoriasis: Correlation with disease severity","volume":"57","author":"Hodeib","year":"2018","journal-title":"Int. J. Dermatol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/s41591-018-0027-5","article-title":"Targeting metabolism to treat psoriasis","volume":"24","author":"Hiebert","year":"2018","journal-title":"Nat. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/1523-1747.ep12477880","article-title":"Increased IL-6 Production by Monocytes and Keratinocytes in Patients with Psoriasis","volume":"97","author":"Neuner","year":"1991","journal-title":"J. Investig. Dermatol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/1523-1747.ep12478128","article-title":"Upper Keratinocytes of Psoriatic Skin Lesions Express High Levels of NAP-1\/IL-8 mRNA In Situ","volume":"97","author":"Gillitzer","year":"1991","journal-title":"J. Investig. Dermatol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Mathes, S.H., Ruffner, H., and Graf-Hausner, U. (2014). The use of skin models in drug development. Adv. Drug Deliv. Rev., 81\u2013102.","DOI":"10.1016\/j.addr.2013.12.006"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jdermsci.2015.07.009","article-title":"Effects of adalimumab, etanercept and ustekinumab on the expression of psoriasin (S100A7) in psoriatic skin","volume":"80","author":"Trovato","year":"2015","journal-title":"J. Dermatol. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Zhang, X., Yin, M., and Zhang, L.-J. (2019). Keratin 6, 16 and 17\u2014Critical Barrier Alarmin Molecules in Skin Wounds and Psoriasis. Cells, 8.","DOI":"10.3390\/cells8080807"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1002\/ptr.6032","article-title":"Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris","volume":"32","author":"Lee","year":"2018","journal-title":"Phytother. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/ddg.12615","article-title":"Importance of basic therapy in psoriasis","volume":"13","author":"Augustin","year":"2015","journal-title":"J. Dtsch. Dermatol. Ges."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ejphar.2017.07.040","article-title":"Imiquimod-induced psoriasis-like inflammation in differentiated Human keratinocytes: Its evaluation using curcumin","volume":"813","author":"Varma","year":"2017","journal-title":"Eur. J. Pharmacol."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Sun, J., Zhao, Y., and Hu, J. (2013). Curcumin Inhibits Imiquimod-Induced Psoriasis-Like Inflammation by Inhibiting IL-1beta and IL-6 Production in Mice. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0067078"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.jdermsci.2009.02.007","article-title":"Anti-psoriatic effects of indigo naturalis on the proliferation and differentiation of keratinocytes with indirubin as the active component","volume":"54","author":"Lin","year":"2009","journal-title":"J. Dermatol. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e349","DOI":"10.1038\/emm.2017.81","article-title":"Rhododendrin inhibits toll-like receptor-7-mediated psoriasis-like skin inflammation in mice","volume":"49","author":"Jeon","year":"2017","journal-title":"Exp. Mol. Med."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"6335","DOI":"10.1038\/s41598-018-24726-z","article-title":"The Therapeutic Potential and Molecular Mechanism of Isoflavone Extract against Psoriasis","volume":"8","author":"Li","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1134\/S1022795414100123","article-title":"Genetically predetermined limitation in HaCaT cells that affects their ability to serve as an experimental model of psoriasis","volume":"50","author":"Soboleva","year":"2014","journal-title":"Russ. J. Genet."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1111\/j.1365-2133.1995.tb02696.x","article-title":"Keratins (Kl6 and Kl7) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro","volume":"133","author":"Leigh","year":"1995","journal-title":"Br. J. Dermatol."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Zhang, W., Dang, E., Shi, X., Jin, L., Feng, Z., Hu, L., Wu, Y., and Wang, G. (2012). The Pro-Inflammatory Cytokine IL-22 Up-Regulates Keratin 17 Expression in Keratinocytes via STAT3 and ERK1\/2. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0040797"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1038\/jid.2011.222","article-title":"IL-17A Upregulates Keratin 17 Expression in Keratinocytes through STAT1- and STAT3-Dependent Mechanisms","volume":"131","author":"Shi","year":"2011","journal-title":"J. Investig. Dermatol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1007\/BF00371788","article-title":"Keratin 17: A useful marker in anti-psoriatic therapies","volume":"283","author":"Ramaekers","year":"1991","journal-title":"Arch. Dermatol. Res."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"4328","DOI":"10.1128\/MCB.20.12.4328-4339.2000","article-title":"Novel Mechanism of Steroid Action in Skin through Glucocorticoid Receptor Monomers","volume":"20","author":"Radoja","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"14026","DOI":"10.1016\/S0021-9258(17)38679-9","article-title":"Coordinate control by vitamin A of keratin gene expression in human keratinocytes","volume":"260","author":"Gilfix","year":"1985","journal-title":"J. Biol. Chem."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1159\/000056350","article-title":"Dithranol and Dimethylfumarate Suppress the Interferon-\u03b3-Induced Up-Regulation of Cytokeratin 17 as a Putative Psoriasis Autoantigen in vitro","volume":"14","author":"Bonnekoh","year":"2001","journal-title":"Ski. Pharmacol. Physiol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1002\/med.21291","article-title":"Keratin 17: A Critical Player in the Pathogenesis of Psoriasis","volume":"34","author":"Jin","year":"2014","journal-title":"Med. Res. Rev."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1111\/j.1600-0625.2010.01235.x","article-title":"Inhibition of keratin 17 expression with antisense and RNAi strategies: Exploring novel therapy for psoriasis","volume":"20","author":"Chang","year":"2011","journal-title":"Exp. Dermatol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.intimp.2018.09.048","article-title":"Gallic acid inhibits the expression of keratin 16 and keratin 17 through Nrf2 in psoriasis-like skin disease","volume":"65","author":"Zhang","year":"2018","journal-title":"Int. Immunopharmacol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1038\/sj.jid.5700599","article-title":"Antimicrobial Peptides Human \u03b2-Defensins Stimulate Epidermal Keratinocyte Migration, Proliferation and Production of Proinflammatory Cytokines and Chemokines","volume":"127","author":"Niyonsaba","year":"2007","journal-title":"J. Investig. Dermatol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"e2344","DOI":"10.1038\/cddis.2016.201","article-title":"Luteolin-7-glucoside inhibits IL-22\/STAT3 pathway, reducing proliferation, acanthosis, and inflammation in keratinocytes and in mouse psoriatic model","volume":"7","author":"Palombo","year":"2016","journal-title":"Cell Death Dis."},{"key":"ref_87","first-page":"648","article-title":"Interleukin-22, a TH17 cytokine, mediates IL-23-induced dermal inflammation and acanthosis","volume":"445","author":"Zheng","year":"2006","journal-title":"Nat. Cell Biol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/nm1162","article-title":"Stat3 links activated keratinocytes and immunocytes required for development of psoriasis in a novel transgenic mouse model","volume":"11","author":"Sano","year":"2005","journal-title":"Nat. Med."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s00403-018-1879-8","article-title":"The role of PI3K\/AKT\/FOXO signaling in psoriasis","volume":"311","author":"Zhang","year":"2019","journal-title":"Arch. Dermatol. Res."},{"key":"ref_90","first-page":"1","article-title":"Capsaicin-loaded vesicular systems designed for enhancing localized delivery for psoriasis therapy","volume":"44","author":"Gupta","year":"2014","journal-title":"Artif. Cell Nanomed. Biotechnol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/j.1468-3083.2009.03377.x","article-title":"A prospective, randomized clinical trial comparing topical aloe vera with 0.1% triamcinolone acetonide in mild to moderate plaque psoriasis","volume":"24","author":"Choonhakarn","year":"2010","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"218","DOI":"10.3889\/oamjms.2018.055","article-title":"Use of Curcumin in Psoriasis","volume":"6","author":"Gianfaldoni","year":"2018","journal-title":"Open Access Maced. J. Med. Sci."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"215","DOI":"10.4103\/0022-3859.201423","article-title":"The impact of topical Saint John\u2019s Wort (Hypericum perforatum) treatment on tissue tumor necrosis factor-alpha levels in plaque-type psoriasis: A pilot study","volume":"63","author":"Hashemian","year":"2017","journal-title":"J. Postgrad. Med."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/j.phymed.2014.02.013","article-title":"Efficacy and safety of Indigo naturalis extract in oil (Lindioil) in treating nail psoriasis: A randomized, observer-blind, vehicle-controlled trial","volume":"21","author":"Lin","year":"2014","journal-title":"Phytomedicine"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1097\/00045391-200603000-00007","article-title":"Treatment of Mild to Moderate Psoriasis with Reli\u00e9va, a Mahonia aquifolium Extract\u2014A Double-Blind, Placebo-Controlled Study","volume":"13","author":"Bernstein","year":"2006","journal-title":"Am. J. Ther."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1055\/s-0042-115177","article-title":"Topically Used Herbal Products for the Treatment of Psoriasis\u2014Mechanism of Action, Drug Delivery, Clinical Studies","volume":"82","author":"Herman","year":"2016","journal-title":"Planta Med."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Hoffmann, J., Gendrisch, F., Schempp, C.M., and W\u00f6lfle, U. (2020). New Herbal Biomedicines for the Topical Treatment of Dermatological Disorders. Biomedicines, 8.","DOI":"10.3390\/biomedicines8020027"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1001\/archderm.144.11.1457","article-title":"Clinical Assessment of Patients with Recalcitrant Psoriasis in a Randomized, Observer-Blind, Vehicle-Controlled Trial Using Indigo Naturalis","volume":"144","author":"Lin","year":"2008","journal-title":"Arch. Dermatol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1440-0960.2012.00877.x","article-title":"The evaluation of the clinical effect of topical St Johns wort (Hypericum perforatum L.) in plaque type psoriasis vulgaris: A pilot study","volume":"53","author":"Najafizadeh","year":"2012","journal-title":"Australas. J. Dermatol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.1002\/j.1460-2075.1989.tb08570.x","article-title":"HPV16 E6 and E7 Proteins Cooperate to Immortalize Human Foreskin Keratinocytes","volume":"8","author":"Vousden","year":"1989","journal-title":"EMBO J."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1039\/c003962d","article-title":"Comparative Quantitation of Proteome Alterations Induced by Aging or Immortalization in Primary Human Fibroblasts and Keratinocytes for Clinical Applications","volume":"6","author":"Sprenger","year":"2010","journal-title":"Mol. BioSyst."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/3\/371\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:31:17Z","timestamp":1760160677000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/3\/371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,2]]},"references-count":101,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["biom11030371"],"URL":"https:\/\/doi.org\/10.3390\/biom11030371","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,2]]}}}