{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:54:51Z","timestamp":1760234091704,"version":"build-2065373602"},"reference-count":78,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T00:00:00Z","timestamp":1616371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Live-stock and Poultry","award":["2018-143"],"award-info":[{"award-number":["2018-143"]}]},{"name":"Modern Agricultural Industry Technology System of\u2002Guangdong\u2002Province","award":["2019KJ127"],"award-info":[{"award-number":["2019KJ127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Endometrial receptivity represents one of the leading factors affecting the successful implantation of embryos during early pregnancy. However, the mechanism of microRNAs (miRNAs) to establish goat endometrial receptivity remains unclear. This study was intended to identify potential miRNAs and regulatory mechanisms associated with establishing endometrial receptivity through integrating bioinformatics analysis and experimental verification. MiRNA expression profiles were obtained by high-throughput sequencing, resulting in the detection of 33 differentially expressed miRNAs (DEMs), followed by their validation through quantitative RT-PCR. Furthermore, 10 potential transcription factors (TFs) and 1316 target genes of these DEMs were obtained, and the TF\u2013miRNA and miRNA\u2013mRNA interaction networks were constructed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that these miRNAs were significantly linked to establishing endometrial receptivity. Moreover, the fluorescence in situ hybridization (FISH) analysis, dual-luciferase report assay, and immunohistochemistry (IHC) analysis corroborated that chi-miR-483 could directly bind to deltex E3 ubiquitin ligase 3L (DTX3L) to reduce its expression level. In conclusion, our findings contribute to a better understanding of molecular mechanisms regulating the endometrial receptivity of goats, and they provide a reference for improving embryo implantation efficiency.<\/jats:p>","DOI":"10.3390\/biom11030472","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T11:13:26Z","timestamp":1616411606000},"page":"472","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats"],"prefix":"10.3390","volume":"11","author":[{"given":"Xupeng","family":"Zang","sequence":"first","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianyu","family":"Gan","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9211-0244","authenticated-orcid":false,"given":"Yaokun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dewu","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangbin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6554-7918","authenticated-orcid":false,"given":"Linjun","family":"Hong","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,22]]},"reference":[{"key":"ref_1","first-page":"39","article-title":"Cellular interactions during implantation in domestic ruminants","volume":"49","author":"Guillomot","year":"1995","journal-title":"J. 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