{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T01:15:29Z","timestamp":1773105329358,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T00:00:00Z","timestamp":1617667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 109-2320-B-182-032-MY3"],"award-info":[{"award-number":["MOST 109-2320-B-182-032-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou","doi-asserted-by":"publisher","award":["CMRPD1H0343"],"award-info":[{"award-number":["CMRPD1H0343"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou","doi-asserted-by":"publisher","award":["CMRPD1H0443"],"award-info":[{"award-number":["CMRPD1H0443"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Administration of albendazole alone was not very suitable for the treatment of cerebral angiostrongyliasis. This study was designed to evaluate the effects of the co-therapy of this drug and dexamethasone in Th-1 and Th-2 dominant mice infected with Angiostrongylus cantonensis. Each of BALB\/c and C57BL\/6 mice infected with 50 A. cantonensis third-stage larvae were administered albendazole (10 mg\/kg\/day) alone, dexamethasone (0.5 mg\/kg\/day) alone, or co-therapy of the two drugs from day 7 or 14 post-infection for 7 or 14 days. After sacrifice, coronal slices were prepared from five brain regions and stained with hematoxylin and eosin. Eight pathological changes were employed to determine the therapeutic effectiveness using a scoring system. RNA-seq analysis was performed to confirm the histopathological findings. The infected BALB\/c and C57BL\/6 mice had similar patterns in the pathological changes. Meningitis, hemorrhage, size of worms, and encephalitis in the cerebral parenchyma were slighter in the mice treated with co-therapy than the remaining groups. Mice treated from day 14 had more severe changes than those from day 7. The histopathological findings were found to be consistent to immune responses determined by RNA-seq analysis. Co-therapy was determined to reduce pathological changes after administration to mice infected with A. cantonensis.<\/jats:p>","DOI":"10.3390\/biom11040536","type":"journal-article","created":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T21:44:47Z","timestamp":1617745487000},"page":"536","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses"],"prefix":"10.3390","volume":"11","author":[{"given":"Kai-Yuan","family":"Jhan","sequence":"first","affiliation":[{"name":"Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien-Ju","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Ming","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Pathology, Chang-Gung Memorial Hospital, Chang-Gung Children Hospital at Linkou and Chang-Gung University, Taoyuan 333, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Jen","family":"Lai","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuang-Yao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Medicine, China Medical University, Taichung 404, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lian-Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"},{"name":"Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"},{"name":"Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/S1473-3099(08)70229-9","article-title":"Human angiostrongyliasis","volume":"8","author":"Wang","year":"2008","journal-title":"Lancet Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s10096-011-1328-5","article-title":"Human Angiostrongylus cantonensis: An update","volume":"31","author":"Wang","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1017\/S0031182016000652","article-title":"Angiostrongylus cantonensis: A review of its distribution, molecular biology and clinical significance as a human pathogen","volume":"143","author":"Barratt","year":"2016","journal-title":"Parasitology"},{"key":"ref_4","first-page":"35","article-title":"Clinical aspects of eosinophilic meningitis and meningoencephalitis caused by Angiostrongylus cantonensis, the rat lungworm","volume":"72","author":"Murphy","year":"2013","journal-title":"Hawaii J. Med. Public Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"191","DOI":"10.4269\/ajtmh.1988.39.191","article-title":"Larvicidal effect of albendazole against Angiostrongylus cantonensis in mice","volume":"39","author":"Hwang","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s00436-004-1105-9","article-title":"The efficacy of therapy with albendazole in mice with parasitic meningitis caused by Angiostrongylus cantonensis","volume":"93","author":"Lan","year":"2004","journal-title":"Parasitol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1093\/jac\/dki430","article-title":"Pathological changes in the brains of rabbits experimentally infected with Angiostrongylus cantonensis after albendazole treatment: Histopathological and magnetic resonance imaging studies","volume":"57","author":"Wang","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1093\/jac\/dkl492","article-title":"Biochemical and pathological evaluation of albendazole\/thalidomide co-therapy against eosinophilic meningitis or meningoencephalitis induced by Angiostrongylus cantonensis","volume":"59","author":"Chen","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1017\/S0022149X10000337","article-title":"Efficacy of combined treatment with albendazole and baicalein against eosinophilic meningitis induced by Angiostrongylus cantonensis in mice","volume":"85","author":"He","year":"2011","journal-title":"J. Helminthol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.exppara.2005.11.017","article-title":"Angiostrongylus cantonensis: Efficacy of albendazole-dexamethasone co-therapy against infection-induced plasminogen activators and eosinophilic meningitis","volume":"113","author":"Tu","year":"2006","journal-title":"Exp. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1186\/s13071-015-0792-7","article-title":"Dexamethasone inhibits brain apoptosis in mice with eosinophilic meningitis caused by Angiostrongylus cantonensis infection","volume":"8","author":"Tsai","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.exppara.2009.04.016","article-title":"Angiostrongylus cantonensis: Effect of combination therapy with albendazole and dexamethasone on Th cytokine gene expression in PBMC from patients with eosinophilic meningitis","volume":"123","author":"Diao","year":"2009","journal-title":"Exp. Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1179\/136485910X12851868780342","article-title":"Treatment of angiostrongyliasis using a combination of albendazole and dexamethasone: The results of a retrospective and comparative study","volume":"105","author":"Diao","year":"2011","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.exppara.2015.08.006","article-title":"Temporal-spatial pathological changes in the brains of permissive and non-permissive hosts experimentally infected with Angiostrongylus cantonensis","volume":"157","author":"Wang","year":"2015","journal-title":"Exp. Parasitol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.jmii.2018.10.010","article-title":"Temporal-spatial expressions of interleukin-4, interleukin-10, and interleukin-13 in the brains of C57BL\/6 and BALB\/c mice infected with Angiostrongylus cantonensis: An immunohistochemical study","volume":"53","author":"Wang","year":"2020","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1189\/jlb.0604341","article-title":"BALB\/c mice have more CD4+CD25+ T regulatory cells and show greater susceptibility to suppression of their CD4+CD25- responder T cells than C57BL\/6 mice","volume":"78","author":"Chen","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Jovicic, N., Jeftic, I., Jovanovic, I., Radosavljevic, G., Arsenijevic, N., Lukic, M.L., and Pejnovic, N. (2015). Differential immunometabolic phenotype in Th1 and Th2 dominant mouse strains in response to high-fat feeding. PLoS One, 10.","DOI":"10.1371\/journal.pone.0134089"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/s41467-017-00050-4","article-title":"Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis","volume":"8","author":"Sahraeian","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.mib.2017.01.001","article-title":"New RNA-seq approaches for the study of bacterial pathogens","volume":"35","author":"Saliba","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.coviro.2018.03.001","article-title":"The use of single-cell RNA-Seq to understand virus-host interactions","volume":"29","author":"Cristinelli","year":"2018","journal-title":"Curr. Opin. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2012.06.021","article-title":"Exploring the host transcriptome for mechanisms underlying protective immunity and resistance to nematode infections in ruminants","volume":"190","author":"Li","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1016\/j.biotechadv.2013.07.006","article-title":"Insights into the immuno-molecular biology of Angiostrongylus vasorum through transcriptomics\u2014prospects for new interventions","volume":"31","author":"Ansell","year":"2015","journal-title":"Biotechnol. Adv."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ijpddr.2014.09.005","article-title":"Drug resistance analysis by next generation sequencing in Leishmania","volume":"5","author":"Leprohon","year":"2014","journal-title":"Int. J. Parasitol. Drugs. Drug. Resist."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2398","DOI":"10.3389\/fimmu.2019.02398","article-title":"Understanding Plasmodium falciparum asymptomatic infections: A proposition for a transcriptomic approach","volume":"10","author":"Kimenyi","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.ygeno.2019.06.015","article-title":"Close encounters between Trypanosoma cruzi and the host mammalian cell: Lessons from genome-wide expression studies","volume":"112","author":"Oliveira","year":"2020","journal-title":"Genomics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"41574","DOI":"10.1038\/srep41574","article-title":"Anti-apoptotic effects of Sonic hedgehog signalling through oxidative stress reduction in astrocytes co-cultured with excretory-secretory products of larval Angiostrongylus cantonensis","volume":"7","author":"Chen","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/nrn2722","article-title":"The Allen Brain Atlas: 5 years and beyond","volume":"10","author":"Jones","year":"2009","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1038\/nprot.2013.099","article-title":"Count-based differential expression analysis of RNA sequencing data using R and Bioconductor","volume":"8","author":"Anders","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"35224","DOI":"10.1038\/srep35224","article-title":"Identification of candidate genes associated with porcine meat color traits by genome-wide transcriptome analysis","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3389\/fgene.2016.00164","article-title":"In papyro comparison of TMM (edgeR), RLE (DESeq2), and MRN normalization methods for a simple two-conditions-without-replicates RNA-seq experimental design","volume":"7","author":"Maza","year":"2016","journal-title":"Front. Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1001\/jama.1962.03050080032007","article-title":"Eosinophilic meningoencephalitis caused by a metastrongylid lung-worm of rats","volume":"179","author":"Rosen","year":"1962","journal-title":"JAMA"},{"key":"ref_32","first-page":"220","article-title":"Pathological findings in four cases of human angiostrongyliasis","volume":"9","author":"Sonakul","year":"1978","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_33","first-page":"262","article-title":"Eosinophilic myelomeningo-encephalitis caused by Angiostrongylus cantonensis: A report of three cases","volume":"22","author":"Witoonpanich","year":"1991","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"541","DOI":"10.5694\/j.1326-5377.1999.tb127880.x","article-title":"A fatal case of angiostrongyliasis in an 11-month-old infant","volume":"170","author":"Kornberg","year":"1999","journal-title":"Med. J. Aust."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"425","DOI":"10.4269\/ajtmh.2004.70.425","article-title":"Fatal autochthonous eosinophilic meningitis in a Jamaican child caused by Angiostrongylus cantonensis","volume":"70","author":"Lindo","year":"2004","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1093\/cid\/cit444","article-title":"Severe hemorrhagic meningoencephalitis due to Angiostrongylus cantonensis among young children in Sydney, Australia","volume":"57","author":"Morton","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1354\/vp.36-4-321","article-title":"Pathological and immunological findings of athymic nude and congenic wild type BALB\/c mice experimentally infected with Neospora caninum","volume":"36","author":"Shibahara","year":"1999","journal-title":"Vet. Pathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.ijdevneu.2007.09.004","article-title":"Long-term histological outcome after post-hypoxic treatment with 100% or 40% oxygen in a model of perinatal hypoxic-ischemic brain injury","volume":"26","author":"Grafe","year":"2008","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1007\/s00436-012-2995-6","article-title":"Differences of larval development and pathological changes in permissive and nonpermissive rodent hosts for Angiostrongylus cantonensis infection","volume":"111","author":"OuYang","year":"2012","journal-title":"Parasitol Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.actatropica.2014.10.002","article-title":"Central nervous system manifestations of Angiostrongylus cantonensis infection","volume":"141","author":"Martins","year":"2015","journal-title":"Acta Trop."},{"key":"ref_41","first-page":"536","article-title":"Anthelmintic effect of levamisole against Angiostrongylus cantonensis in mice","volume":"10","author":"Hwang","year":"1994","journal-title":"Kaohsiung. J. Med. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1007\/s00436-012-3228-8","article-title":"Efficacy of tribendimidine against Angiostrongylus cantonensis infection in the mice","volume":"112","author":"Wang","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"512","DOI":"10.2307\/3281499","article-title":"Studies on anthelmintic effects of flubendazole and mebendazole on the rat lungworm Angiostrongylus cantonensis in mice and rats","volume":"72","author":"Maki","year":"1986","journal-title":"J. Parasitol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1017\/S0022149X00025839","article-title":"Larvicidal effect of flubendazole on Angiostrongylus cantonensis in mice with various worm burdens","volume":"59","author":"Maki","year":"1985","journal-title":"J. Helminthol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1093\/jac\/41.2.145","article-title":"Albendazole","volume":"41","author":"Venkatesan","year":"1998","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_46","first-page":"345","article-title":"Animal model of human disease. Central nervous system infection with the nematode Angiostrongylus cantonenis. Animal model: Eosinophilic meningoencephalitis in mice infected with Angiostrongylus cantonensis","volume":"80","author":"John","year":"1975","journal-title":"Am. J. Pathol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1079\/JOH2004256","article-title":"Induced eosinophilia and proliferation in Angiostrongylus cantonensis-infected mouse brain are associated with the induction of JAK\/STAT1, IAP\/NF-kappaB and MEKK1\/JNK signals","volume":"78","author":"Lan","year":"2004","journal-title":"J. Helminthol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-015-1214-6","article-title":"Effects of albendazole combined with TSII-A (a Chinese herb compound) on optic neuritis caused by Angiostrongylus cantonensis in BALB\/c mice","volume":"8","author":"Feng","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1056\/NEJM199805283382206","article-title":"Eosinophilia","volume":"338","author":"Rothenberg","year":"1998","journal-title":"N. Engl. J. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1086\/314036","article-title":"Corticosteroid treatment of eosinophilic meningitis","volume":"31","author":"Chotmongkol","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.amjmed.2004.06.038","article-title":"A 1-week course of corticosteroids in the treatment of eosinophilic meningitis","volume":"117","author":"Sawanyawisuth","year":"2004","journal-title":"Am. J. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s00436-016-5322-9","article-title":"Spleen atrophy related immune system changes attributed to infection of Angiostrongylus cantonensis in mouse model","volume":"116","author":"Liu","year":"2016","journal-title":"Parasitol. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1186\/s12974-016-0743-z","article-title":"Activation of the hypothalamic-pituitary-adrenal (HPA) axis contributes to the immunosuppression of mice infected with Angiostrongylus cantonensis","volume":"13","author":"Chen","year":"2016","journal-title":"J. Neuroinflamm."},{"key":"ref_54","first-page":"46","article-title":"A severe case of rat lungworm disease in Hawa\u2019i","volume":"72","author":"Howe","year":"2013","journal-title":"Hawaii J. Med. Public Health"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/BF00314741","article-title":"Dexamethasone increases plasma levels of albendazole","volume":"237","author":"Jung","year":"1990","journal-title":"J. Neurol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/0020-7519(92)90122-2","article-title":"The influence of Mesocestoides corti on subsequent Angiostrongylus cantonensis infections in mice","volume":"22","author":"Yoshimura","year":"1992","journal-title":"Int. J. Parasitol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/s004360050199","article-title":"Cytokine responses in mice infected with Angiostrongylus cantonensis","volume":"83","author":"Sugaya","year":"1997","journal-title":"Parasitol. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s004360050363","article-title":"The role of CD4+ and CD8+ T-cells in host morbidity and innate resistance to Angiostrongylus cantonensis in the mouse","volume":"84","author":"Aoki","year":"1998","journal-title":"Parasitol. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1016\/j.drudis.2018.05.015","article-title":"Next-generation sequencing in drug development: Target identification and genetically stratified clinical trials","volume":"23","author":"Torshizi","year":"2018","journal-title":"Drug Discov. Today"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1186\/s12936-019-2880-1","article-title":"Applying next-generation sequencing to track falciparum malaria in sub-Saharan Africa","volume":"18","author":"Tessema","year":"2019","journal-title":"Malar. J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1186\/s12936-019-2853-4","article-title":"Deployment and utilization of next-generation sequencing of Plasmodium falciparum to guide anti-malarial drug policy decisions in sub-Saharan Africa: Opportunities and challenges","volume":"18","author":"Ishengoma","year":"2019","journal-title":"Malar. J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s11259-014-9614-1","article-title":"Developmental profiles of GFAP-positive astrocytes in sheep cerebellum","volume":"38","author":"Salouci","year":"2014","journal-title":"Vet. Res. Commun."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.exppara.2010.04.017","article-title":"Oxidative stress in mice infected with Angiostrongylus cantonensis coincides with enhanced glutathione-dependent enzymes activity","volume":"126","author":"Chung","year":"2010","journal-title":"Exp. Parasitol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1186\/s13071-015-0939-6","article-title":"Preliminary expression profile of cytokines in brain tissue of BALB\/c mice with Angiostrongylus cantonensis infection","volume":"8","author":"Yu","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s12974-018-1071-2","article-title":"Chi3l3: A potential key orchestrator of eosinophil recruitment in meningitis induced by Angiostrongylus cantonensis","volume":"15","author":"Wan","year":"2018","journal-title":"J. Neuroinflammation"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/4\/536\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:11:27Z","timestamp":1760364687000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/4\/536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,6]]},"references-count":65,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["biom11040536"],"URL":"https:\/\/doi.org\/10.3390\/biom11040536","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,6]]}}}