{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T14:30:37Z","timestamp":1762353037397,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>We examined the effects of two histone acetyltransferase (HAT) inhibitors on the activity of rice serotonin N-acetyltransferases (SNAT). Two rice recombinant SNAT isoenzymes (SNAT1 and SNAT2) were incubated in the presence of either MG149 or MB3, HAT inhibitors. MG149 significantly inhibited the SNAT enzymes in a dose-dependent manner, especially SNAT1, while SNAT2 was moderately inhibited. By contrast, MB3 had no effect on SNAT1 or SNAT2. The application of 100 \u03bcM MG149 to rice seedlings decreased melatonin by 1.6-fold compared to the control, whereas MB3 treatment did not alter the melatonin level. MG149 significantly decreased both melatonin and N-acetylserotonin when rice seedlings were challenged with cadmium, a potent elicitor of melatonin synthesis in rice. Although MG149 inhibited melatonin synthesis in rice seedlings, no melatonin deficiency-induced lamina angle decrease was observed due to the insufficient suppression of SNAT2, which is responsible for the lamina angle decrease in rice.<\/jats:p>","DOI":"10.3390\/biom11050658","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T10:30:41Z","timestamp":1619692241000},"page":"658","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Inhibition of Rice Serotonin N-Acetyltransferases by MG149 Decreased Melatonin Synthesis in Rice Seedlings"],"prefix":"10.3390","volume":"11","author":[{"given":"Kyungjin","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geun-Hee","family":"Choi","sequence":"additional","affiliation":[{"name":"Nakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3795-9747","authenticated-orcid":false,"given":"Kyoungwhan","family":"Back","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/j.1600-079X.1995.tb00136.x","article-title":"Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry","volume":"18","author":"Dubbels","year":"1995","journal-title":"J. Pineal Res."},{"key":"ref_2","first-page":"627","article-title":"Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates","volume":"35","author":"Hattori","year":"1995","journal-title":"Biochem. Mol. Biol. Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4677","DOI":"10.1093\/jxb\/eraa235","article-title":"An evolutionary view of melatonin synthesis and metabolism related to its biological functions in plant","volume":"71","author":"Tan","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/tpj.14915","article-title":"Melatonin metabolism, signaling and possible roles in plants","volume":"105","author":"Back","year":"2021","journal-title":"Plant J."},{"doi-asserted-by":"crossref","unstructured":"Hwang, O.J., and Back, K. (2020). Simultaneous suppression of two distinct serotonin N-acetyltransferase isogenes by RNA interference leads to severe decreases in melatonin and accelerated seed deterioration in rice. Biomolecules, 10.","key":"ref_5","DOI":"10.3390\/biom10010141"},{"doi-asserted-by":"crossref","unstructured":"Xiao, S., Liu, L., Wang, H., Li, D., Bai, Z., Zhang, Y., Sun, H., Zhang, K., and Li, C. (2019). Exogenous melatonin accelerates seed germination in cotton (Gossypium hirsutum L.). PLoS ONE, 14.","key":"ref_6","DOI":"10.1101\/618959"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e12537","DOI":"10.1111\/jpi.12537","article-title":"Melatonin-deficient rice plants show a common semidwarf phenotype either dependent or independent of brassinosteroid biosynthesis","volume":"66","author":"Lee","year":"2019","journal-title":"J. Pineal Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s00497-020-00388-8","article-title":"Melatonin in flowering, fruit set and fruit ripening","volume":"33","author":"Arnao","year":"2020","journal-title":"Plant Reprod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e12392","DOI":"10.1111\/jpi.12392","article-title":"Overexpression of rice serotonin N-acetyltransferase 1 in transgenic rice plants confers resistance to cadmium and senescence and increases grain yield","volume":"62","author":"Lee","year":"2017","journal-title":"J. Pineal Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.tplants.2018.10.010","article-title":"Melatonin: A new plant hormone and\/or a plant master regulator?","volume":"24","author":"Arnao","year":"2019","journal-title":"Trends Plant Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10","DOI":"10.32794\/mr11250029","article-title":"Melatonin in the evolution of plants and other phototrophs","volume":"2","author":"Hardeland","year":"2019","journal-title":"Melatonin Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s11105-016-1015-x","article-title":"The effects of melatonin on transcriptional profile of unfolded protein response genes under endoplasmic reticulum stress in Arabidopsis thaliana","volume":"35","author":"Ozgur","year":"2017","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"93","DOI":"10.32794\/mr11250006","article-title":"Melatonin plays a pivotal role in conferring tolerance against endoplasmic reticulum stress via mitogen-activated protein kinases and bZIP60 in Arabidopsis thaliana","volume":"1","author":"Lee","year":"2018","journal-title":"Melatonin Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1111\/tpj.14477","article-title":"Melatonin enhances cotton immunity to Verticillium wilt via manipulating lignin and gossypol biosynthesis","volume":"100","author":"Li","year":"2019","journal-title":"Plant J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1104\/pp.20.00117","article-title":"Melatonin represses oil and anthocyanin accumulation in seeds","volume":"183","author":"Li","year":"2020","journal-title":"Plant Physiol."},{"doi-asserted-by":"crossref","unstructured":"Moustafa-Farag, M., Elkelish, A., Dafea, M., Khan, M., Arnao, M.B., Abdelhamid, M.T., El-Ezz, A.A., Almoneafy, A., Mahmoud, A., and Awad, M. (2020). Role of melatonin in plant tolerance to soil stressors: Salinity, pH and heavy metals. Molecules, 25.","key":"ref_16","DOI":"10.3390\/molecules25225359"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1094\/PDIS-11-19-2361-RE","article-title":"Exogenous melatonin enhances rice plant resistance against Xanthomonas oryzae pv. oryzae","volume":"104","author":"Chen","year":"2020","journal-title":"Plant Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"70","DOI":"10.32794\/mr11250082","article-title":"Salt stress in Arabidopsis thaliana seedlings: Role of indoleamines in stress alleviation","volume":"4","author":"Shukla","year":"2021","journal-title":"Melatonin Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e12500","DOI":"10.1111\/jpi.12500","article-title":"Phytomelatonin receptor PMTR1-mediated signaling regulates stomatal closure in Arabidopsis thaliana","volume":"65","author":"Wei","year":"2018","journal-title":"J. Pineal Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"177","DOI":"10.32794\/mr11250055","article-title":"The phytomelatonin receptor (PMRT1) Arabidopsis Cand2 is not a bona fide G protein-coupled melatonin receptor","volume":"3","author":"Lee","year":"2020","journal-title":"Melatonin Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"152","DOI":"10.32794\/11250036","article-title":"Melatonin and reactive oxygen and nitrogen species: A model for the plant redox network","volume":"2","author":"Arnao","year":"2019","journal-title":"Melatonin Res."},{"doi-asserted-by":"crossref","unstructured":"Khan, A., Numan, M., Khan, A.L., Lee, I.J., Imran, M., Asaf, S., and Al-Harrasi, A. (2020). Melatonin: Awakening the defense mechanisms during plant oxidative stress. Plants, 9.","key":"ref_22","DOI":"10.3390\/plants9040407"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/jpi.12053","article-title":"Transcriptional suppression of tryptamine 5-hydroxylase, a terminal serotonin biosynthetic gene, induces melatonin biosynthesis in rice (Oryza sativa L.)","volume":"55","author":"Park","year":"2013","journal-title":"J. Pineal Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1021\/cr500452k","article-title":"Protein lysine acetylation by p300\/CBP","volume":"115","author":"Dancy","year":"2015","journal-title":"Chem. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.drudis.2013.11.012","article-title":"Small molecule inhibitors of histone acetyltransferases and deacetylases are potential drugs for inflammatory diseases","volume":"19","author":"Dekker","year":"2014","journal-title":"Drug Discov. Today"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.bbrc.2016.12.086","article-title":"Chemical inhibition of the histone acetyltransferase activity in Arabidopsis thaliana","volume":"483","author":"Aquea","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s10725-018-0460-y","article-title":"Histone acetylation modification affects cell wall degradation and aerenchyma formation in wheat seminal roots under waterlogging","volume":"87","author":"Li","year":"2018","journal-title":"Plant Growth Reg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1093\/pcp\/pcaa018","article-title":"SISNAT interacts with HSP40, a molecular chaperone, to regulate melatonin biosynthesis and promote thermotolerance in tomato","volume":"61","author":"Wang","year":"2020","journal-title":"Plant Cell Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/jpi.12011","article-title":"Molecular cloning of rice serotonin N-acetyltransferase, the penultimate gene in plant melatonin biosynthesis","volume":"55","author":"Kang","year":"2013","journal-title":"J. Pineal Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1111\/jpi.12339","article-title":"Cloning and characterization of the serotonin N-acetyltransferase-2 gene (SNAT2) in rice (Oryza sativa)","volume":"61","author":"Byeon","year":"2016","journal-title":"J. Pineal Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/jpi.12103","article-title":"Cellular localization and kinetics of the rice melatonin biosynthetic enzymes SNAT and ASMT","volume":"56","author":"Byeon","year":"2014","journal-title":"J. Pineal Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1046\/j.1365-313X.2002.01438.x","article-title":"Loss-of-function of a rice brassinosteroid biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem","volume":"32","author":"Hong","year":"2002","journal-title":"Plant J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s10265-014-0665-8","article-title":"Circadian expression profiles of chromatin remodeling factor genes in Arabidopsis","volume":"128","author":"Lee","year":"2015","journal-title":"J. Plant Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.ejmech.2011.11.001","article-title":"6-alkylsalicylates are selective Tip60 inhibitors and target the acetyl-CoA binding site","volume":"47","author":"Ghizzoni","year":"2012","journal-title":"Eur. J. Med. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e12441","DOI":"10.1111\/jpi.12441","article-title":"Cadmium-induced melatonin synthesis in rice requires light, hydrogen peroxide, and nitric oxide: Key regulatory roles for tryptophan decarboxylase and caffeic acid O-methyltransferase","volume":"63","author":"Lee","year":"2017","journal-title":"J. Pineal Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1111\/jpi.12232","article-title":"Coordinated regulation of melatonin synthesis and degradation genes in rice leaves in response to cadmium treatment","volume":"58","author":"Byeon","year":"2015","journal-title":"J. Pineal Res."},{"doi-asserted-by":"crossref","unstructured":"Hwang, O.J., and Back, K. (2019). Melatonin deficiency confers tolerance to multiple abiotic stresses in rice via decreased brassinosteroid levels. Int. J. Mol. Sci., 20.","key":"ref_37","DOI":"10.3390\/ijms20205173"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.mce.2006.03.039","article-title":"Evolution of arylalkylamine N-acetyltransferase: Emergence and divergence","volume":"252","author":"Coon","year":"2006","journal-title":"Mol. Cell Endocrinol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5372","DOI":"10.1038\/srep05372","article-title":"Rational design and validation of a Tip60 histone acetyltransferase inhibitor","volume":"4","author":"Gao","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6195","DOI":"10.1021\/ja981365a","article-title":"A potent inhibitor of the melatonin rhythm enzyme","volume":"120","author":"Khalil","year":"1998","journal-title":"J. Am. Chem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.bbrc.2013.09.076","article-title":"The flavonoid myricetin reduces nocturnal melatonin levels in the blood through the inhibition of serotonin N-acetyltransferase","volume":"440","author":"Shin","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e12512","DOI":"10.1111\/jpi.12512","article-title":"Flavonoids inhibit both rice and sheep serotonin N-acetyltransferases and reduce melatonin levels in plants","volume":"65","author":"Lee","year":"2018","journal-title":"J. Pineal Res."},{"doi-asserted-by":"crossref","unstructured":"Lee, H.Y., and Back, K. (2021). Melatonin regulates chloroplast protein quality control via a mitogen-activated protein kinase signaling pathway. Antioxidants, 10.","key":"ref_43","DOI":"10.3390\/antiox10040511"},{"doi-asserted-by":"crossref","unstructured":"Hwang, O.J., and Back, K. (2021). Suppression of rice cryptochrome 1b decreases both melatonin and expression of brassinosteroid biosynthetic genes resulting in salt tolerance. Molecules, 26.","key":"ref_44","DOI":"10.3390\/molecules26041075"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/5\/658\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:55:29Z","timestamp":1760162129000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/5\/658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,29]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["biom11050658"],"URL":"https:\/\/doi.org\/10.3390\/biom11050658","relation":{},"ISSN":["2218-273X"],"issn-type":[{"type":"electronic","value":"2218-273X"}],"subject":[],"published":{"date-parts":[[2021,4,29]]}}}