{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T21:28:39Z","timestamp":1782163719681,"version":"3.54.5"},"reference-count":39,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T00:00:00Z","timestamp":1621209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Cm-p5 is a snail-derived antimicrobial peptide, which demonstrated antifungal activity against the pathogenic strains of Candida albicans. Previously we synthetized a cyclic monomer as well as a parallel and an antiparallel dimer of Cm-p5 with improved antifungal activity. Considering the alarming increase of microbial resistance to conventional antibiotics, here we evaluated the antimicrobial activity of these derivatives against multiresistant and problematic bacteria and against important viral agents. The three peptides showed a moderate activity against Pseudomonas aeruginosa, Klebsiella pneumoniae\u00a0Extended Spectrum \u03b2-Lactamase (ESBL), and Streptococcus agalactiae, with MIC values &gt; 100 \u00b5g\/mL. They exerted a considerable activity with MIC values between 25\u201350 \u00b5g\/mL against Acinetobacter baumanii and Enterococcus faecium. In addition, the two dimers showed a moderate activity against Pseudomonas aeruginosa PA14. The three Cm-p5 derivatives inhibited a virulent extracellular strain of Mycobacterium tuberculosis, in a dose-dependent manner. Moreover, they inhibited Herpes Simplex Virus 2 (HSV-2) infection in a concentration-dependent manner, but had no effect on infection by the Zika Virus (ZIKV) or pseudoparticles of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2). At concentrations of &gt;100 \u00b5g\/mL, the three new Cm-p5 derivatives showed toxicity on different eukaryotic cells tested. Considering a certain cell toxicity but a potential interesting activity against the multiresistant strains of bacteria and HSV-2, our compounds require future structural optimization.<\/jats:p>","DOI":"10.3390\/biom11050745","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T12:19:57Z","timestamp":1621253997000},"page":"745","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains"],"prefix":"10.3390","volume":"11","author":[{"given":"Melaine","family":"Gonz\u00e1lez-Garc\u00eda","sequence":"first","affiliation":[{"name":"Center for Protein Studies, Faculty of Biology, University of Havana, 25 St, La Habana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fidel","family":"Morales-Vicente","sequence":"additional","affiliation":[{"name":"General Chemistry Department, Faculty of Chemistry and Center for Natural Products Research, Faculty of Chemistry, University of Havana, Zapata street, La Habana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erbio D\u00edaz","family":"Pico","sequence":"additional","affiliation":[{"name":"Synthetic Peptides Group, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, La Habana 10600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilda","family":"Garay","sequence":"additional","affiliation":[{"name":"Synthetic Peptides Group, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, La Habana 10600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Havana University, Zapata Street, La Habana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Grieshober","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Clinic of Ulm, Albert-Einstein-Allee 23, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lia","family":"Raluca Olari","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0355-7915","authenticated-orcid":false,"given":"R\u00fcdiger","family":"Gro\u00df","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina","family":"Conzelmann","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Zech","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Prelli Bozzo","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janis A.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Zelikin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Aarhus University, Langelandsgade 140, 8000 Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz","family":"Raber","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, Ulm University, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Kubiczek","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, Ulm University, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9297-6419","authenticated-orcid":false,"given":"Frank","family":"Rosenau","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, Ulm University, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7316-7141","authenticated-orcid":false,"given":"Jan","family":"M\u00fcnch","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology, University Clinic of Ulm, Meyerhofstrasse. 1, 89081 Ulm, Germany"},{"name":"Core Facility for Functional Peptidomics, Ulm Peptide Pharmaceuticals (U-PEP), Faculty of Medicine, Ulm University, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Stenger","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Clinic of Ulm, Albert-Einstein-Allee 23, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Spellerberg","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Clinic of Ulm, Albert-Einstein-Allee 23, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9546-0525","authenticated-orcid":false,"given":"Octavio L.","family":"Franco","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Catholic University Dom Bosco, Campo Grande and Center for Biochemical and Proteomics Analyses, Catholic University of Brasilia, Brasilia, DF 71966-700, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5921-3770","authenticated-orcid":false,"given":"Armando A.","family":"Rodriguez Alfonso","sequence":"additional","affiliation":[{"name":"Core Facility for Functional Peptidomics, Ulm 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Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3315","DOI":"10.1096\/fj.14-269860","article-title":"Cm-p5: An antifungal hydrophilic peptide derived from the coastal mollusk Cenchritis muricatus (Gastropoda: Littorinidae)","volume":"29","author":"McBeth","year":"2015","journal-title":"FASEB J."},{"key":"ref_2","first-page":"1","article-title":"In vitro complementary biological activity of the antifungal peptide Cm-p5 and in silico prediction of its functional regions","volume":"69","author":"Freitas","year":"2017","journal-title":"Rev. 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