{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T17:58:40Z","timestamp":1774461520712,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,3]],"date-time":"2021-10-03T00:00:00Z","timestamp":1633219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["19-08-00874-a"],"award-info":[{"award-number":["19-08-00874-a"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["20-015-00147-a"],"award-info":[{"award-number":["20-015-00147-a"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>In disease states, mesothelial cells are exposed to variable osmotic conditions, with high osmotic stress exerted by peritoneal dialysis (PD) fluids. They contain unphysiologically high concentrations of glucose and result in major peritoneal membrane transformation and PD function loss. The effects of isotonic entry of urea and myo-inositol in hypertonic (380 mOsm\/kg) medium on the cell volume of primary cultures of rat peritoneal mesothelial cells and rat kidney outer medullary collecting duct (OMCD) principal cells were studied. In hypertonic medium, rat peritoneal mesothelial cells activated a different mechanism of cell volume regulation in the presence of isotonic urea (100 mM) in comparison to rat kidney OMCD principal cells. In kidney OMCD cells inflow of urea into the shrunken cell results in restoration of cell volume. In the shrunken peritoneal mesothelial cells, isotonic urea inflow caused a small volume increase and activated regulatory volume decrease (RVD). Isotonic myo-inositol activated RVD in hypertonic medium in both cell types. Isotonic application of both osmolytes caused a sharp increase of intracellular calcium both in peritoneal mesothelial cells and in kidney OMCD principal cells. In conclusion, peritoneal mesothelial cells exhibit RVD mechanisms when challenged with myo-inositol and urea under hyperosmolar isotonic switch from mannitol through involvement of calcium-dependent control. Myo-inositol effects were identical with the ones in OMCD principal cells whereas urea effects in OMCD principal cells led to no RVD induction.<\/jats:p>","DOI":"10.3390\/biom11101452","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T09:24:15Z","timestamp":1633339455000},"page":"1452","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparison of Isotonic Activation of Cell Volume Regulation in Rat Peritoneal Mesothelial Cells and in Kidney Outer Medullary Collecting Duct Principal Cells"],"prefix":"10.3390","volume":"11","author":[{"given":"Galina S.","family":"Baturina","sequence":"first","affiliation":[{"name":"Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia"},{"name":"Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liubov E.","family":"Katkova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus Peter","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgeniy I.","family":"Solenov","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia"},{"name":"Department of Physiology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Department of Data Acquisition and Analysis, Novosibirsk State Technical University, 630087 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3083-3244","authenticated-orcid":false,"given":"Sotirios G.","family":"Zarogiannis","sequence":"additional","affiliation":[{"name":"Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany"},{"name":"Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Thessaly, 41500 Larissa, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0005-2736(98)00051-0","article-title":"Differential activation of system A and betaine\/GABA transport in MDCK cell membranes by hypertonic stress","volume":"1372","author":"Kempson","year":"1998","journal-title":"Biochim. Biophys. Acta-Biomembr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1152\/advan.00034.2004","article-title":"Cellular volume homeostasis","volume":"28","author":"Strange","year":"2004","journal-title":"Adv. Physiol. Educ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1152\/physrev.00056.2006","article-title":"Cellular response to hyperosmotic stresses","volume":"87","author":"Burg","year":"2007","journal-title":"Physiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1152\/physrev.00037.2007","article-title":"Physiology of cell volume regulation in vertebrates","volume":"89","author":"Hoffmann","year":"2009","journal-title":"Physiol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/nrm.2016.29","article-title":"VRACs and other ion channels and transporters in the regulation of cell volume and beyond","volume":"17","author":"Jentsch","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Lindinger, M.I., Leung, M.J., and Hawke, T.J. (2013). Inward Flux of Lactate- through Monocarboxylate Transporters Contributes to Regulatory Volume Increase in Mouse Muscle Fibres. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0084451"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1159\/000366380","article-title":"The Water Permeability Reduction After Successive Hypo-Osmotic Shocks in Kidney Principal Cells is Apically Regulated","volume":"34","author":"Katkova","year":"2014","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1007\/s00249-003-0308-9","article-title":"Effect of dDAVP on basolateral cell surface water permeability in the outer medullary collecting duct","volume":"32","author":"Solenov","year":"2003","journal-title":"Eur. Biophys. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1111\/1440-1681.12666","article-title":"Brattleboro rats have impaired apical membrane water permeability regulation in the outer medullary collecting duct principal cells","volume":"4","author":"Baturina","year":"2016","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.mbs.2013.05.007","article-title":"Regulatory volume decrease of rat kidney principal cells after successive hypo-osmotic shocks","volume":"244","author":"Zarogiannis","year":"2013","journal-title":"Math. Biosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1085\/jgp.106.3.525","article-title":"Regulatory volume decrease in cultured kidney cells (A6): Role of amino acids","volume":"106","author":"Simaels","year":"1995","journal-title":"J. Gen. Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.1007\/s11538-017-0338-6","article-title":"Cell Volume Regulation in the Proximal Tubule of Rat Kidney: Proximal Tubule Cell Volume Regulation","volume":"79","author":"Edwards","year":"2017","journal-title":"Bull. Math. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"869","DOI":"10.33594\/000000060","article-title":"Benign pleural mesothelial cells have higher osmotic water permeability than malignant pleural mesothelioma cells and differentially respond to hyperosmolality","volume":"52","author":"Katkova","year":"2019","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"103581","DOI":"10.1016\/j.resp.2020.103581","article-title":"Pleural effusion osmolality correlation with pH and glucose level of pleural fluid and its effects on the pleural membrane permeability","volume":"285","author":"Peppa","year":"2021","journal-title":"Respir. Physiol. Neurobiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.3389\/fphys.2018.01853","article-title":"Biocompatible peritoneal dialysis: The target is still way off","volume":"9","author":"Bartosova","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1007\/s00467-011-1863-4","article-title":"Solutions for peritoneal dialysis in children: Recommendations by the European Pediatric Dialysis Working Group","volume":"26","author":"Schmitt","year":"2011","journal-title":"Pediatr. Nephrol."},{"key":"ref_17","first-page":"21344","article-title":"Quantitative Histomorphometry of the Healthy Peritoneum","volume":"6","author":"Schaefer","year":"2016","journal-title":"Nat. Publ. Gr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"108","DOI":"10.2215\/CJN.00690112","article-title":"Article Effect of the Dialysis Fluid Buffer on Peritoneal Membrane Function in Children","volume":"8","author":"Schmitt","year":"2013","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.kint.2018.02.022","article-title":"Neutral pH and low\u2013glucose degradation product dialysis fluids induce major early alterations of the peritoneal membrane in children on peritoneal dialysis","volume":"94","author":"Schaefer","year":"2018","journal-title":"Kidney Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"261813","DOI":"10.3389\/fphys.2015.00244","article-title":"Animal models in peritoneal dialysis","volume":"6","author":"Nikitidou","year":"2015","journal-title":"Front. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"C426","DOI":"10.1152\/ajpcell.00298.2003","article-title":"Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method","volume":"286","author":"Solenov","year":"2004","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.4149\/gpb_2013063","article-title":"Quantitative estimation of transmembrane ion transport in rat renal collecting duct principal cells","volume":"33","author":"Ilyaskin","year":"2014","journal-title":"Gen. Physiol. Biophys."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/978-94-024-1057-0_18","article-title":"Methods to measure water permeability","volume":"969","author":"Solenov","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wilson, C.S., and Mongin, A.A. (2018). Cell Volume Control in Healthy Brain and Neuropathologies, Elsevier Ltd.","DOI":"10.1016\/bs.ctm.2018.07.006"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1111\/apha.12450","article-title":"The identification of a volume-regulated anion channel: An amazing Odyssey","volume":"213","author":"Pedersen","year":"2015","journal-title":"Acta Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.2215\/CJN.08750812","article-title":"Urine-concentrating mechanism in the inner medulla: Function of the thin limbs of the loops of henle","volume":"9","author":"Dantzler","year":"2014","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.2215\/CJN.04480413","article-title":"Thick ascending limb of the loop of henle","volume":"9","author":"Mount","year":"2014","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Rahman, S., Park, J., and Kim, J. (2017). Osmolytes offset the urea\u2019s effect on protein structure and function. Cellular Osmolytes: From Chaperoning Protein Folding to Clinical Perspectives, Springer.","DOI":"10.1007\/978-981-10-3707-8_4"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"232","DOI":"10.3389\/fphys.2014.00232","article-title":"Role of [Ca2+]i and F-actin on mesothelial barrier function","volume":"5","author":"Kuwahara","year":"2014","journal-title":"Front. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1093\/ndt\/14.5.1217","article-title":"Response to hypertonicity in mesothelial cells: Role of Na+\/myo-inositol co-transporter","volume":"14","author":"Matsuoka","year":"1999","journal-title":"Nephrol. Dial. Transplant."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/10\/1452\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:09:10Z","timestamp":1760166550000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/10\/1452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,3]]},"references-count":30,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["biom11101452"],"URL":"https:\/\/doi.org\/10.3390\/biom11101452","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,3]]}}}