{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:56:37Z","timestamp":1792788997239,"version":"4.5.0"},"reference-count":81,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T00:00:00Z","timestamp":1633564800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science Technology, Taiwan","award":["MOST107-2622-B-033-001-CC2 and MOST108-2622-B-033-001-CC2 to C.-D.H. and MOST109-2313-B-034-003- and MOST110-2313-B-034-002- to Y.-H.L."],"award-info":[{"award-number":["MOST107-2622-B-033-001-CC2 and MOST108-2622-B-033-001-CC2 to C.-D.H. and MOST109-2313-B-034-003- and MOST110-2313-B-034-002- to Y.-H.L."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Cardiac arrhythmia has been defined as one of the abnormal heart rhythm symptoms, which is a common problem dealt with by cardiologists. Zebrafish were established as a powerful animal model with a transparent body that enables optical observation to analyze cardiac morphology and cardiac rhythm regularity. Currently, research has observed heart-related parameters in zebrafish, which used different approaches, such as starting from the use of fluorescent transgenic zebrafish, different software, and different observation methods. In this study, we developed an innovative approach by using the OpenCV library to measure zebrafish larvae heart rate and rhythm. The program is designed in Python, with the feature of multiprocessing for simultaneous region-of-interest (ROI) detection, covering both the atrium and ventricle regions in the video, and was designed to be simple and user-friendly, having utility even for users who are unfamiliar with Python. Results were validated with our previously published method using ImageJ, which observes pixel changes. In summary, the results showed good consistency in heart rate-related parameters. In addition, the established method in this study also can be widely applied to other invertebrates (like Daphnia) for cardiac rhythm measurement.<\/jats:p>","DOI":"10.3390\/biom11101476","type":"journal-article","created":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T09:24:04Z","timestamp":1633598644000},"page":"1476","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["An OpenCV-Based Approach for Automated Cardiac Rhythm Measurement in Zebrafish from Video Datasets"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4735-7069","authenticated-orcid":false,"given":"Ali","family":"Farhan","sequence":"first","affiliation":[{"name":"Department of Chemistry, Chung Yuan Christian University, Chung-Li 320314, Taiwan"},{"name":"Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3403-0879","authenticated-orcid":false,"given":"Kevin Adi","family":"Kurnia","sequence":"additional","affiliation":[{"name":"Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5715-9827","authenticated-orcid":false,"given":"Ferry","family":"Saputra","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chung Yuan Christian University, Chung-Li 320314, Taiwan"},{"name":"Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2709-2633","authenticated-orcid":false,"given":"Kelvin H.-C.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, National Pingtung University, Pingtung 900391, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3997-1683","authenticated-orcid":false,"given":"Jong-Chin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, National Pingtung University, Pingtung 900391, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6398-1828","authenticated-orcid":false,"given":"Marri Jmelou M.","family":"Roldan","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, The Graduate School, University of Santo Tomas, Espana Blvd., Manila 1015, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Heng","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6398-8672","authenticated-orcid":false,"given":"Chung-Der","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chung Yuan Christian University, Chung-Li 320314, Taiwan"},{"name":"Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, Taiwan"},{"name":"Center for Nanotechnology, Chung Yuan Christian University, Chung-Li 320314, Taiwan"},{"name":"Research Center for Aquatic Toxicology and Pharmacology, Chung Yuan Christian University, Chung-Li 320314, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,7]]},"reference":[{"key":"ref_1","unstructured":"American Heart Association (2021, June 30). 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