{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:16:17Z","timestamp":1785906977194,"version":"3.56.0"},"reference-count":64,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007619","name":"Osteology Foundation","doi-asserted-by":"publisher","award":["16-045"],"award-info":[{"award-number":["16-045"]}],"id":[{"id":"10.13039\/501100007619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n (COLCIENCIAS)","doi-asserted-by":"publisher","award":["2017-756"],"award-info":[{"award-number":["2017-756"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>This study aimed to analyze the effects of fibrin constructs enhanced with laminin-nidogen, implanted in the wounded rat soft palate. Fibrin constructs with and without laminin-nidogen were implanted in 1 mm excisional wounds in the soft palate of 9-week-old rats and compared with the wounded soft palate without implantation. Collagen deposition and myofiber formation were analyzed at days 3, 7, 28 and 56 after wounding by histochemistry. In addition, immune staining was performed for a-smooth muscle actin (a-SMA), myosin heavy chain (MyHC) and paired homeobox protein 7 (Pax7). At day 56, collagen areas were smaller in both implant groups (31.25 \u00b1 7.73% fibrin only and 21.11 \u00b1 6.06% fibrin with laminin-nidogen)) compared to the empty wounds (38.25 \u00b1 8.89%, p &lt; 0.05). Moreover, the collagen area in the fibrin with laminin-nidogen group was smaller than in the fibrin only group (p \u02c2 0.05). The areas of myofiber formation in the fibrin only group (31.77 \u00b1 10.81%) and fibrin with laminin-nidogen group (43.13 \u00b1 10.39%) were larger than in the empty wounds (28.10 \u00b1 11.68%, p \u02c2 0.05). Fibrin-based constructs with laminin-nidogen reduce fibrosis and improve muscle regeneration in the wounded soft palate. This is a promising strategy to enhance cleft soft palate repair and other severe muscle injuries.<\/jats:p>","DOI":"10.3390\/biom11101547","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T01:23:46Z","timestamp":1634693026000},"page":"1547","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury"],"prefix":"10.3390","volume":"11","author":[{"given":"Doris H.","family":"Rosero Salazar","sequence":"first","affiliation":[{"name":"Department of Dentistry, Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre, 6525EX Nijmegen, The Netherlands"},{"name":"Department of Medical Basic Sciences, Faculty of Health, Universidad Icesi, Cali 760008, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 E. M.","family":"van Rheden","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre, 6525EX Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"van Hulzen","sequence":"additional","affiliation":[{"name":"Central Facility for Research with Laboratory Animals (CDL), Radboud University Medical Centre, 6525EZ Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola L.","family":"Carvajal Monroy","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Special Dental Care and Orthodontics, Erasmus Medical Center, 3015GD Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4837-1559","authenticated-orcid":false,"given":"Frank A. D. T. 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