{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T04:54:41Z","timestamp":1772772881994,"version":"3.50.1"},"reference-count":13,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Experimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from Rhizopus oryzae suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the particular behavior of the extraction system in the presence of fungal biomass. The interfacial mass flow of the reaction product was found to be significantly affected by the biomass, due mainly to its adsorption onto the phase separation interface, this leading to the appearance of a physical barrier against the solute\u2019s transfer. However, the magnitude of the adsorption phenomenon was found to depend on Rhizopus oryzae\u2019s affinity for the solvent phase, which increased significantly from dichloromethane to n-heptane. The negative influence of the biomass on the interfacial mass transfer can be partially counteracted by adding 1-octanol into the organic phase, improving the solvent\u2019s ability to solve the fumaric acid\u2013Amberlite LA-2 complex and simplifying the reactive extraction mechanism, effects that were found to be more important for low-polar solvents. Consequently, for the same mixing intensity, the maximum amplification factor was reached for n-heptane, its value being almost 5\u20136 times higher than that obtained for dichloromethane and over 2 times higher than that obtained with n-butyl acetate.<\/jats:p>","DOI":"10.3390\/biom11111563","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T14:37:48Z","timestamp":1634827068000},"page":"1563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions\u2014Interfacial Mass Transfer"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6396-0813","authenticated-orcid":false,"given":"Dan","family":"Cascaval","sequence":"first","affiliation":[{"name":"Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2365-6670","authenticated-orcid":false,"given":"Anca-Irina","family":"Galaction","sequence":"additional","affiliation":[{"name":"Faculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy, M. Kogalniceanu 9-13, 700454 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Tucaliuc","sequence":"additional","affiliation":[{"name":"Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenuta","family":"Kloetzer","sequence":"additional","affiliation":[{"name":"Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1080\/07388551.2019.1620677","article-title":"Bioproduction of fumaric acid: An insight into microbial strain improvement strategies","volume":"39","author":"Sebastian","year":"2019","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s10529-018-2628-y","article-title":"Fumaric acid, production and separation","volume":"41","author":"Ilica","year":"2019","journal-title":"Biotechnol. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1002\/jctb.1590","article-title":"Organic acids: Old metabolites, new themes","volume":"81","author":"Goldberg","year":"2006","journal-title":"Chem. Technol. 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Quart."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/11\/1563\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:20:10Z","timestamp":1760167210000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/11\/11\/1563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,21]]},"references-count":13,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["biom11111563"],"URL":"https:\/\/doi.org\/10.3390\/biom11111563","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,21]]}}}