{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:19:33Z","timestamp":1790605173418,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI16\/01404, PI13\/2594, PI20\/01069, RD16\/0017\/0018, and RD16\/0007\/0006"],"award-info":[{"award-number":["PI16\/01404, PI13\/2594, PI20\/01069, RD16\/0017\/0018, and RD16\/0007\/0006"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Background and aim: Glycomic alterations serve as biomarker tools for different diseases. The present study aims to evaluate the diagnostic capability of serum N-glycosylation to identify alcohol risk drinking in comparison with standard markers. Methods: We included 1516 adult individuals (age range 18\u201391 years; 55.3% women), randomly selected from a general population. A total of 143 (21.0%) men and 50 (5.9%) women were classified as risk drinkers after quantification of daily alcohol consumption and the Alcohol Use Disorders Identification Test (AUDIT). Hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) was used for the quantification of 46 serum N-glycan peaks. Serum gamma-glutamyltransferase (GGT), carbohydrate-deficient transferrin (CDT), and red blood cell mean corpuscular volume (MCV) were measured by standard clinical laboratory methods. Results: Variations in serum N-glycome associated risk drinking were more prominent in men compared to women. A unique combination of N-glycan peaks selected by the selbal algorithm shows good discrimination between risk-drinkers and non-risk drinkers for men and women. Receiver operating characteristics (ROC) curves show accuracy for the diagnosis of risk drinking, which is comparable to that of the golden standards, GGT, MCV and CDT markers for men and women. Additionally, the inclusion of N-glycan peaks improves the diagnostic accuracy of the standard markers, although it remains relatively low, due to low sensitivity. For men, the area under the ROC curve using N-glycome data is 0.75, 0.76, and 0.77 when combined with GGT, MCV, and CDT, respectively. In women, the areas were 0.76, 0.73, and 0.73, respectively. Conclusion: Risk drinking is associated with significant variations in the serum N-glycome, which highlights its potential diagnostic utility.<\/jats:p>","DOI":"10.3390\/biom12020241","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T09:59:21Z","timestamp":1643709561000},"page":"241","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Changes in Serum N-Glycome for Risk Drinkers: A Comparison with Standard Markers for Alcohol Abuse in Men and Women"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1941-4775","authenticated-orcid":false,"given":"R\u00f3is\u00edn","family":"O\u2019Flaherty","sequence":"first","affiliation":[{"name":"GlycoScience Group, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, A94 X099 Co. Dublin, Ireland"},{"name":"Department of Chemistry, Maynooth University, Maynooth, W23 F2K8 Co. Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9504-8773","authenticated-orcid":false,"given":"\u00c1d\u00e1m","family":"Simon","sequence":"additional","affiliation":[{"name":"GlycoScience Group, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, A94 X099 Co. Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Alonso-Sampedro","sequence":"additional","affiliation":[{"name":"Grupo de Metodolog\u00eda de la Investigaci\u00f3n, Instituto de Investigaci\u00f3n Sanitaria (IDIS), Complejo Hospitalario Universitario, Santiago de Compostela, 15706 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"S\u00e1nchez-Bat\u00e1n","sequence":"additional","affiliation":[{"name":"Grupo de Metodolog\u00eda de la Investigaci\u00f3n, Instituto de Investigaci\u00f3n Sanitaria (IDIS), Complejo Hospitalario Universitario, Santiago de Compostela, 15706 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Fern\u00e1ndez-Merino","sequence":"additional","affiliation":[{"name":"Primary Care Center, A Estrada, 36680 Pontevedra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9681-1662","authenticated-orcid":false,"given":"Francisco","family":"Gude","sequence":"additional","affiliation":[{"name":"Grupo de Metodolog\u00eda de la Investigaci\u00f3n, Instituto de Investigaci\u00f3n Sanitaria (IDIS), Complejo Hospitalario Universitario, Santiago de Compostela, 15706 Santiago de Compostela, Spain"},{"name":"Department of Clinical Epidemiology, Complejo Hospitalario Universitario, Santiago de Compostela, 15706 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5085-5080","authenticated-orcid":false,"given":"Radka","family":"Saldova","sequence":"additional","affiliation":[{"name":"GlycoScience Group, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, A94 X099 Co. Dublin, Ireland"},{"name":"UCD School of Medicine, College of Health and Agricultural Science, University College Dublin, D04 V1W8 Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Gonz\u00e1lez-Quintela","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Complejo Hospitalario Universitario, University of Santiago de Compostela, 15706 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2018). Global Status Report on Alcohol and Health 2018, World Health Organization."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1515\/CCLM.2007.190","article-title":"Biochemical markers of alcoholism","volume":"45","author":"Hannuksela","year":"2007","journal-title":"Clin. Chem. Lab. Med. 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