{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T01:56:30Z","timestamp":1791510990095,"version":"4.3.4"},"reference-count":23,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100020648","name":"State University of Santa Cruz","doi-asserted-by":"publisher","award":["073.11006.2019.0009828-54"],"award-info":[{"award-number":["073.11006.2019.0009828-54"]}],"id":[{"id":"10.13039\/501100020648","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Sickle leg ulcers (SLU) are malleoli lesions with exuberant hemolytic pathophysiology. The microRNAs are potential genetic biomarkers for several pathologies. Thereby, we aimed to assess the expression of circulating miR-199a-5p, miR-144, and miR-126 in association with hemolytic biomarkers in SLU. This cross-sectional study included 69 patients with sickle cell disease, 52 patients without SLU (SLU-) and 17 patients with active SLU or previous history (SLU+). The results demonstrated elevated expression of circulating miR-199a-5p and miR-144 in SLU+ patients while miR-126 expression was reduced. Circulating miR-199a-5p and miR-144 were associated with hemolytic biomarkers such as LDH, indirect bilirubin, AST, GGT, iron, ferritin, RBC, hemoglobin, and NOm, in addition to association with impaired clinical profile of SLU. Furthermore, in silico analyses indicated interactions of miR-199a-5p with HIF1A, Ets-1, and TGFB2 genes, which are associated with vasculopathy and reduced NO. In contrast, miR-126 was associated with an attenuating clinical profile of SLU, in addition to not characterizing hemolysis. In summary, this study demonstrates, for the first time, that hemolytic mechanism in SLU can be characterized by circulating miR-199a-5p and miR-144. The circulating miR-126 may play a protective role in SLU. Thus, these microRNAs can support to establish prognosis and therapeutic strategy in SLU.<\/jats:p>","DOI":"10.3390\/biom12020317","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T20:24:26Z","timestamp":1645129466000},"page":"317","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Description of the Hemolytic Component in Sickle Leg Ulcer: The Role of Circulating miR-199a-5p, miR-144, and miR-126"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2265-1544","authenticated-orcid":false,"given":"Edvan do Carmo","family":"Santos","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Patologia Aplicada e Gen\u00e9tica, Departamento de Ci\u00eancias Biol\u00f3gicas, Universidade Estadual de Santa Cruz, Ilh\u00e9us 45662-900, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Imbassahy Valentim","family":"Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Aplicada e Gen\u00e9tica, Departamento de Ci\u00eancias Biol\u00f3gicas, Universidade Estadual de Santa Cruz, Ilh\u00e9us 45662-900, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Vin\u00edcius Bispo","family":"Santana","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Aplicada e Gen\u00e9tica, Departamento de Ci\u00eancias Biol\u00f3gicas, Universidade Estadual de Santa Cruz, Ilh\u00e9us 45662-900, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idaiara Graziele Silva","family":"Quadros","sequence":"additional","affiliation":[{"name":"Centro de Refer\u00eancia a Doen\u00e7a Falciforme de Itabuna, Itabuna 45600-075, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e8tondji Cocou Modeste 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Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Magalh\u00e3es","family":"Fiuza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o em Gen\u00e9tica e Hematologia Translacional, Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador 40296-710, BA, Brazil"},{"name":"Laborat\u00f3rio de Pesquisa em Anemias, Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade Federal da Bahia, Salvador 40170-115, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u00e9llen Pinheiro","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o em Gen\u00e9tica e Hematologia Translacional, Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador 40296-710, BA, Brazil"},{"name":"Laborat\u00f3rio de Pesquisa em Anemias, Departamento de An\u00e1lises Cl\u00ednicas e 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Cardoso","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias da Sa\u00fade, Universidade Estadual de Santa Cruz, Ilh\u00e9us 45662-900, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilda Souza","family":"Goncalves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o em Gen\u00e9tica e Hematologia Translacional, Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Oswaldo Cruz, Salvador 40296-710, BA, Brazil"},{"name":"Laborat\u00f3rio de Pesquisa em Anemias, Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade Federal da Bahia, Salvador 40170-115, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6928-824X","authenticated-orcid":false,"given":"Milena Magalh\u00e3es","family":"Aleluia","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia 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