{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:10:18Z","timestamp":1790021418725,"version":"4.0.1"},"reference-count":51,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T00:00:00Z","timestamp":1646870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Ischemic stroke is a common cerebrovascular disease and recovering blood flow as early as possible is essential to reduce ischemic damage and maintain neuronal viability, but the reperfusion process usually causes additional damage to the brain tissue in the ischemic area, namely ischemia reperfusion injury. The accumulated studies have revealed that transplantation of exogenous neural stem cells (NSCs) is an ideal choice for the treatment of ischemia reperfusion injury. At present, the source and efficacy of exogenous NSCs after transplantation is still one of the key issues that need to be resolved. In this study, human umbilical cord mesenchymal stem cells (hUC-MSCs) were obtained and induced into NSCs byadding growth factor and neuregulin1\u03b2 (NRG1\u03b2) was introduced during the differentiation process of NSCs. Then, the rat middle cerebral artery occlusion\/reperfusion (MCAO\/R) models were established, and the therapeutic effects were evaluated among groups treated by NRG1\u03b2, NSCs and NSCs pretreated with 10 nM NRG1\u03b2 (NSCs-10 nM NRG1\u03b2) achieved through intra-arterial injection. Our data show that the NSCs-10 nM NRG1\u03b2 group significantly improves neurobehavioral function and infarct volume after MCAO\/R, as well as cerebral cortical neuron injury, ferroptosis-related indexes and mitochondrial injury. Additionally, NSCs-10 nM NRG1\u03b2 intervention may function through regulating the p53\/GPX4\/SLC7A11 pathway, and reducing the level of ferroptosis in cells, further enhance the neuroprotective effect on injured cells.<\/jats:p>","DOI":"10.3390\/biom12030428","type":"journal-article","created":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T11:46:47Z","timestamp":1646912807000},"page":"428","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Transplantation of Human Umbilical Cord Mesenchymal Stem Cells-Derived Neural Stem Cells Pretreated with Neuregulin1\u03b2 Ameliorate Cerebral Ischemic Reperfusion Injury in Rats"],"prefix":"10.3390","volume":"12","author":[{"given":"Qiu-Yue","family":"Zhai","sequence":"first","affiliation":[{"name":"Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Qian","family":"Ren","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin-Shuai","family":"Ni","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Hua","family":"Song","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology, Qingdao Medical College, Qingdao University, Qingdao 266021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke-Li","family":"Ge","sequence":"additional","affiliation":[{"name":"Institute of Integrative Medicine, Qingdao Medical College, Qingdao University, Qingdao 266021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Liang","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1055\/s-0038-1649503","article-title":"Global Burden of Stroke","volume":"38","author":"Katan","year":"2018","journal-title":"Semin. 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