{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:57:32Z","timestamp":1790683052331,"version":"4.1.0"},"reference-count":85,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T00:00:00Z","timestamp":1646956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The distinct neuropathological features of the different \u03b1-Synucleinopathies, as well as the diversity of the \u03b1-Synuclein (\u03b1-Syn) intracellular inclusion bodies observed in post mortem brain sections, are thought to reflect the strain diversity characterizing invasive \u03b1-Syn amyloids. However, this \u201cone strain, one disease\u201d view is still hypothetical, and to date, a possible disease-specific contribution of non-amyloid factors has not been ruled out. In Multiple System Atrophy (MSA), the buildup of \u03b1-Syn inclusions in oligodendrocytes seems to result from the terminal storage of \u03b1-Syn amyloid aggregates first pre-assembled in neurons. This assembly occurs at the level of neuronal cytoplasmic inclusions, and even earlier, within neuronal intranuclear inclusions (NIIs). Intriguingly, \u03b1-Syn NIIs are never observed in \u03b1-Synucleinopathies other than MSA, suggesting that these inclusions originate (i) from the unique molecular properties of the \u03b1-Syn fibril strains encountered in this disease, or alternatively, (ii) from other factors specifically dysregulated in MSA and driving the intranuclear fibrillization of \u03b1-Syn. We report the isolation and structural characterization of a synthetic human \u03b1-Syn fibril strain uniquely capable of seeding \u03b1-Syn fibrillization inside the nuclear compartment. In primary mouse cortical neurons, this strain provokes the buildup of NIIs with a remarkable morphology reminiscent of cat\u2019s eye marbles (see video abstract). These \u03b1-Syn inclusions form giant patterns made of one, two, or three lentiform beams that span the whole intranuclear volume, pushing apart the chromatin. The input fibrils are no longer detectable inside the NIIs, where they become dominated by the aggregation of endogenous \u03b1-Syn. In addition to its phosphorylation at S129, \u03b1-Syn forming the NIIs acquires an epitope antibody reactivity profile that indicates its organization into fibrils, and is associated with the classical markers of \u03b1-Syn pathology p62 and ubiquitin. NIIs are also observed in vivo after intracerebral injection of the fibril strain in mice. Our data thus show that the ability to seed NIIs is a strain property that is integrally encoded in the fibril supramolecular architecture. Upstream alterations of cellular mechanisms are not required. In contrast to the lentiform TDP-43 NIIs, which are observed in certain frontotemporal dementias and which are conditional upon GRN or VCP mutations, our data support the hypothesis that the presence of \u03b1-Syn NIIs in MSA is instead purely amyloid-strain-dependent.<\/jats:p>","DOI":"10.3390\/biom12030436","type":"journal-article","created":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T12:58:36Z","timestamp":1647003516000},"page":"436","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Neurons with Cat\u2019s Eyes: A Synthetic Strain of \u03b1-Synuclein Fibrils Seeding Neuronal Intranuclear Inclusions"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4850-3582","authenticated-orcid":false,"given":"Francesca","family":"De Giorgi","sequence":"first","affiliation":[{"name":"Institut des Maladies Neurod\u00e9g\u00e9n\u00e9ratives, CNRS, UMR 5293, 33076 Bordeaux, France"},{"name":"Institut des Maladies Neurod\u00e9g\u00e9n\u00e9ratives, UMR 5293, Universit\u00e9 de Bordeaux, 33076 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammed Bilal","family":"Abdul-Shukkoor","sequence":"additional","affiliation":[{"name":"Institut de Chimie et de Biologie des Membranes et des Nano-objets, CNRS, UMR 5248, Universit\u00e9 de Bordeaux, 33600 Pessac, France"},{"name":"Institut Europ\u00e9en de Chimie et Biologie, Universit\u00e9 de Bordeaux, 33600 Pessac, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7266-6387","authenticated-orcid":false,"given":"Marianna","family":"Kashyrina","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies, Section of Human Anatomy, University of Salento, 73100 Lecce, 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