{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:37:39Z","timestamp":1789000659772,"version":"build-2803163510"},"reference-count":24,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T00:00:00Z","timestamp":1649721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006686","name":"University of Miami","doi-asserted-by":"publisher","award":["UM SAC 2017-28"],"award-info":[{"award-number":["UM SAC 2017-28"]}],"id":[{"id":"10.13039\/100006686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Background: Preventing delayed cerebral ischemia (DCI) after subarachnoid hemorrhage (SAH) remains an important therapeutic target. Preconditioning stimulates multiple endogenous protective mechanisms and may be a suitable treatment for DCI following SAH. We here compare remote limb conditioning with resveratrol conditioning in a clinically relevant SAH model. Methods: We produced a SAH in 39 male Sprague Dawley rats using a single injection model. Animals were randomized to four groups: repetitive limb conditioning with a blood pressure cuff, sham conditioning, intraperitoneal resveratrol (10 mg\/kg) or intraperitoneal vehicle administered at 24, 48 and 72 h after SAH. On day 4 neurological and behavioral scores were obtained, and animals were euthanized. The cross-sectional area of the basilar artery was measured at the vertebrobasilar junction, and at the mid and distal segments. Hippocampal cells were counted in both hemispheres and normalized per mm length. We compared true limb preconditioning with sham conditioning and resveratrol with vehicle preconditioning. Results: The cross-sectional area of the mid-basilar artery in the true limb preconditioning group was significantly larger by 43% (p = 0.03) when compared with the sham preconditioning group. No differences in the cross-sectional area were found in the resveratrol-treated group when compared to the vehicle-treated group. We found no differences in the neuro score, behavioral score, and in mean hippocampal neuron counts between the groups. Conclusion: We found beneficial vascular effects of remote limb preconditioning on SAH-induced basilar artery vasoconstriction. Our findings support further studies of limb preconditioning as a potential treatment after SAH.<\/jats:p>","DOI":"10.3390\/biom12040568","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T21:15:35Z","timestamp":1649798135000},"page":"568","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage"],"prefix":"10.3390","volume":"12","author":[{"given":"Sebastian","family":"Koch","sequence":"first","affiliation":[{"name":"Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giselle","family":"De La Rua","sequence":"additional","affiliation":[{"name":"Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donnae","family":"Farquharson","sequence":"additional","affiliation":[{"name":"Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Saul","sequence":"additional","affiliation":[{"name":"Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Perez-Pinzon","sequence":"additional","affiliation":[{"name":"Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Neuroscience Program, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunjan","family":"Dave","sequence":"additional","affiliation":[{"name":"Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Neuroscience Program, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.1161\/STROKEAHA.115.010844","article-title":"Location, Infarct Load, and 3-Month Outcomes of Delayed Cerebral Infarction After Aneurysmal Subarachnoid Hemorrhage","volume":"46","author":"Wong","year":"2015","journal-title":"Stroke"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1161\/STROKEAHA.111.000773","article-title":"Preconditioning the human brain: Proving the principle in subarachnoid hemorrhage","volume":"44","author":"Koch","year":"2013","journal-title":"Stroke"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.neuroscience.2009.01.017","article-title":"Resveratrol pretreatment protects rat brain from cerebral ischemic damage via a sirtuin 1-uncoupling protein 2 pathway","volume":"159","author":"Dave","year":"2009","journal-title":"Neuroscience"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.2174\/092986708784638861","article-title":"Resveratrol and ischemic preconditioning in the brain","volume":"15","author":"Raval","year":"2008","journal-title":"Curr. 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