{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:21:42Z","timestamp":1768868502547,"version":"3.49.0"},"reference-count":68,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Regione Lazio","award":["A0375-2020-36555"],"award-info":[{"award-number":["A0375-2020-36555"]}]},{"name":"Regione Lazio","award":["232\/2016"],"award-info":[{"award-number":["232\/2016"]}]},{"name":"MIUR-Italy Departments of Excellence","award":["A0375-2020-36555"],"award-info":[{"award-number":["A0375-2020-36555"]}]},{"name":"MIUR-Italy Departments of Excellence","award":["232\/2016"],"award-info":[{"award-number":["232\/2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Protease inhibitors are widely studied since the unrestricted activity of proteases can cause extensive organ lesions. In particular, elastase activity is involved in the pathophysiology of acute lung injury, for example during SARS-CoV-2 infection, while serine proteases and thrombin-like proteases are involved in the development and\/or pathology of the nervous system. Natural protease inhibitors have the advantage to be reversible and with few side effects and thus are increasingly considered as new drugs. Kunitz-type protease inhibitors (KTPIs), reported in the venom of various organisms, such as wasps, spiders, scorpions, and snakes, have been studied for their potent anticoagulant activity and widespread protease inhibitor activity. Putative KTPI anticoagulants have been identified in transcriptomic resources obtained for two blister beetle species, Lydus trimaculatus and Mylabris variabilis. The KTPIs of L. trimaculatus and M. variabilis were characterized by combined transcriptomic and bioinformatics methodologies. The full-length mRNA sequences were divided on the base of the sequence of the active sites of the putative proteins. In silico protein structure analyses of each group of translational products show the biochemical features of the active sites and the potential protease targets. Validation of these genes is the first step for considering these molecules as new drugs for use in medicine.<\/jats:p>","DOI":"10.3390\/biom12070988","type":"journal-article","created":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T21:00:28Z","timestamp":1658091628000},"page":"988","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Characterization of Kunitz-Type Protease Inhibitors from Blister Beetles (Coleoptera, Meloidae)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2522-1718","authenticated-orcid":false,"given":"Emiliano","family":"Fratini","sequence":"first","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"},{"name":"Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4485-7738","authenticated-orcid":false,"given":"Marianna Nicoletta","family":"Rossi","sequence":"additional","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2831-1383","authenticated-orcid":false,"given":"Lucrezia","family":"Spagoni","sequence":"additional","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3719-9941","authenticated-orcid":false,"given":"Alessandra","family":"Riccieri","sequence":"additional","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emiliano","family":"Mancini","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnologies, \u201cCharles Darwin\u201d, Sapienza University, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7657-2019","authenticated-orcid":false,"given":"Fabio","family":"Polticelli","sequence":"additional","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco Alberto","family":"Bologna","sequence":"additional","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1044-7108","authenticated-orcid":false,"given":"Paolo","family":"Mariottini","sequence":"additional","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8535-8279","authenticated-orcid":false,"given":"Manuela","family":"Cervelli","sequence":"additional","affiliation":[{"name":"Department of Sciences, University of Roma Tre, 00146 Rome, Italy"},{"name":"Neurodevelopment, Neurogenetics and Molecular Neurobiology Unit, IRCCS Fondazione Santa Lucia, Via del Fosso di Fiorano 64, 00143 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"D160","DOI":"10.1093\/nar\/gkh071","article-title":"MEROPS: The peptidase database","volume":"32","author":"Rawlings","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/nrd3053","article-title":"Emerging principles in protease-based drug discovery","volume":"9","author":"Drag","year":"2010","journal-title":"Nat. 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