{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:55:26Z","timestamp":1791597326955,"version":"4.3.4"},"reference-count":54,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM)","award":["R01CA157595"],"award-info":[{"award-number":["R01CA157595"]}]},{"name":"Ligue Nationale contre le Cancer-Comit\u00e9 de Paris (RS22\/75-100)","award":["R01CA157595"],"award-info":[{"award-number":["R01CA157595"]}]},{"name":"National Cancer Institute of the National Institutes of Health","award":["R01CA157595"],"award-info":[{"award-number":["R01CA157595"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Kallikrein-related peptidases (KLKs) are implicated in many cancer-related processes. KLK6, one of the 15 KLK family members, is a promising biomarker for diagnosis of many cancers and has been associated with poor prognosis of colorectal cancer (CRC) patients. Herein, we evaluated the expression and cellular functions of KLK6 in colon cancer-derived cell lines and in clinical samples from CRC patients. We showed that, although many KLKs transcripts are upregulated in colon cancer-derived cell lines, KLK6, KLK10, and KLK11 are the most highly secreted proteins. KLK6 induced calcium flux in HT29 cells by activation and internalization of protease-activated receptor 2 (PAR2). Furthermore, KLK6 induced extracellular signal\u2013regulated kinases 1 and 2 (ERK1\/2) phosphorylation. KLK6 suppression in HCT-116 colon cancer cells decreased the colony formation, increased cell adhesion to extracellular matrix proteins, and reduced spheroid formation and compaction. Immunohistochemistry (IHC) analysis demonstrated ectopic expression of KLK6 in human colon adenocarcinomas but not in normal epithelia. Importantly, high levels of KLK6 protein were detected in the ascites of CRC patients with peritoneal metastasis, but not in benign ascites. These data indicate that KLK6 overexpression is associated with aggressive CRC, and may be applied to differentiate between benign and malignant ascites.<\/jats:p>","DOI":"10.3390\/biom12071003","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T23:10:22Z","timestamp":1658272222000},"page":"1003","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Kallikrein-Related Peptidase 6 (KLK6) as a Contributor toward an Aggressive Cancer Cell Phenotype: A Potential Role in Colon Cancer Peritoneal Metastasis"],"prefix":"10.3390","volume":"12","author":[{"given":"Hayet","family":"Bouzid","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) INSERM-1275, H\u00f4pital Lariboisi\u00e8re, 75010 Paris, France"},{"name":"Universit\u00e9 de Paris Cit\u00e9, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1818-703X","authenticated-orcid":false,"given":"Feryel","family":"Soualmia","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, IBPS, UMR 8256 CNRS-UPMC, ERL INSERM U1164, Biological Adaptation and Ageing, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Oikonomopoulou","sequence":"additional","affiliation":[{"name":"Schroeder Arthritis Institute, Krembil Research Institute, University Health Network, Toronto, ON M5T 3L9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoninus","family":"Soosaipillai","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON M5T 3L9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Pathology, H\u00f4pital Bichat-Claude Bernard, 75018 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaoula","family":"Louati","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) INSERM-1275, H\u00f4pital Lariboisi\u00e8re, 75010 Paris, France"},{"name":"Universit\u00e9 de Paris Cit\u00e9, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rea","family":"Lo Dico","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) INSERM-1275, H\u00f4pital Lariboisi\u00e8re, 75010 Paris, France"},{"name":"Universit\u00e9 de Paris Cit\u00e9, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Pocard","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) INSERM-1275, H\u00f4pital Lariboisi\u00e8re, 75010 Paris, France"},{"name":"Universit\u00e9 de Paris Cit\u00e9, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1028-5113","authenticated-orcid":false,"given":"Chahrazade","family":"El Amri","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, IBPS, UMR 8256 CNRS-UPMC, ERL INSERM U1164, Biological Adaptation and Ageing, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia A.","family":"Ignatenko","sequence":"additional","affiliation":[{"name":"University of Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalila","family":"Darmoul","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) INSERM-1275, H\u00f4pital Lariboisi\u00e8re, 75010 Paris, France"},{"name":"Universit\u00e9 de Paris Cit\u00e9, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7692","DOI":"10.3748\/wjg.v22.i34.7692","article-title":"Pathophysiology of colorectal peritoneal carcinomatosis: Role of the peritoneum","volume":"22","author":"Lemoine","year":"2016","journal-title":"World J. Gastroenterol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7407190","DOI":"10.1155\/2019\/7407190","article-title":"Mechanisms of Metastasis in Colorectal Cancer and Metastatic Organotropism: Hematogenous versus Peritoneal Spread","volume":"2019","author":"Pretzsch","year":"2019","journal-title":"J. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1101\/gad.250647.114","article-title":"Pericellular proteolysis in cancer","volume":"28","author":"Sevenich","year":"2014","journal-title":"Genes Dev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"32592","DOI":"10.18632\/oncotarget.8693","article-title":"The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression","volume":"7","author":"Xie","year":"2016","journal-title":"Oncotarget"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1111\/febs.15829","article-title":"Molecular mechanisms regulating Proteinase-Activated Receptors (PARs)","volume":"288","author":"Chandrabalan","year":"2021","journal-title":"Febs J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1054\/bjoc.2001.1976","article-title":"Initiation of human colon cancer cell proliferation by trypsin acting at protease-activated receptor-2","volume":"85","author":"Darmoul","year":"2001","journal-title":"Br. J. Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/S0002-9440(10)64283-6","article-title":"Aberrant expression and activation of the thrombin receptor protease-activated receptor-1 induces cell proliferation and motility in human colon cancer cells","volume":"162","author":"Darmoul","year":"2003","journal-title":"Am. J. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"20927","DOI":"10.1074\/jbc.M401430200","article-title":"Protease-activated receptor 2 in colon cancer: Trypsin-induced MAPK phosphorylation and cell proliferation are mediated by epidermal growth factor receptor transactivation","volume":"279","author":"Darmoul","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1158\/1541-7786.514.2.9","article-title":"Activation of proteinase-activated receptor 1 promotes human colon cancer cell proliferation through epidermal growth factor receptor transactivation","volume":"2","author":"Darmoul","year":"2004","journal-title":"Mol. Cancer Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.biochi.2015.09.002","article-title":"The kallikrein-related peptidase family: Dysregulation and functions during cancer progression","volume":"122","author":"Kryza","year":"2016","journal-title":"Biochimie"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1160\/TH12-11-0791","article-title":"Kallikrein-related peptidases (KLKs) in gastrointestinal cancer: Mechanistic and clinical aspects","volume":"110","author":"Kontos","year":"2012","journal-title":"Thromb. Haemost."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3109\/10408363.2016.1154643","article-title":"Kallikrein-related peptidases (KLKs) and the hallmarks of cancer","volume":"53","author":"Filippou","year":"2016","journal-title":"Crit. Rev. Clin. Lab. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/s41568-021-00436-z","article-title":"Remodelling of the tumour microenvironment by the kallikrein-related peptidases","volume":"22","author":"Srinivasan","year":"2022","journal-title":"Nat. Rev. Cancer"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.2353\/ajpath.2010.090523","article-title":"Kallikrein-related peptidase 4: A new activator of the aberrantly expressed protease-activated receptor 1 in colon cancer cells","volume":"176","author":"Gratio","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1016\/j.ajpath.2011.07.016","article-title":"Kallikrein-related peptidase 14 acts on proteinase-activated receptor 2 to induce signaling pathway in colon cancer cells","volume":"179","author":"Gratio","year":"2011","journal-title":"Am. J. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1515\/bc.2010.038","article-title":"Kallikrein-related peptidases: Proteolysis and signaling in cancer, the new frontier","volume":"391","author":"Oikonomopoulou","year":"2010","journal-title":"Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1515\/cclm.2011.750","article-title":"The physiology and pathobiology of human kallikrein-related peptidase 6 (KLK6)","volume":"50","author":"Bayani","year":"2012","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1159\/000081393","article-title":"In silico analysis of the human kallikrein gene 6","volume":"25","author":"Yousef","year":"2004","journal-title":"Tumor Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1002\/cncr.25841","article-title":"Upregulation and clinical significance of serine protease kallikrein 6 in colon cancer","volume":"117","author":"Kim","year":"2011","journal-title":"Cancer"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1007\/s13277-014-1612-y","article-title":"Kallikrein-related peptidase-6 (KLK6) mRNA expression is an independent prognostic tissue biomarker of poor disease-free and overall survival in colorectal adenocarcinoma","volume":"35","author":"Christodoulou","year":"2014","journal-title":"Tumor Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1038\/bjc.2012.220","article-title":"Lymph node tissue kallikrein-related peptidase 6 mRNA: A progression marker for colorectal cancer","volume":"107","author":"Ohlsson","year":"2012","journal-title":"Br. J. Cancer."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"17876","DOI":"10.18632\/oncotarget.24947","article-title":"Diagnostic and prognostic biomarker potential of kallikrein family genes in different cancer types","volume":"9","author":"Tailor","year":"2018","journal-title":"Oncotarget"},{"key":"ref_23","first-page":"2021","article-title":"A 65--gene signature for prognostic prediction in colon adenocarcinoma","volume":"41","author":"Jiang","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6062","DOI":"10.18632\/oncotarget.27153","article-title":"Kallikrein 6 protease advances colon tumorigenesis via induction of the high mobility group A2 protein","volume":"10","author":"Chen","year":"2019","journal-title":"Oncotarget"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Pandey, R., Zhou, M., Chen, Y., Darmoul, D., Kisiel, C.C., Nfonsam, V.N., and Ignatenko, N.A. (2021). Molecular Pathways Associated with Kallikrein 6 Overexpression in Colorectal Cancer. Genes, 12.","DOI":"10.3390\/genes12050749"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.neo.2017.02.003","article-title":"Specific microRNA-mRNA Regulatory Network of Colon Cancer Invasion Mediated by Tissue Kallikrein-Related Peptidase 6","volume":"19","author":"Sells","year":"2017","journal-title":"Neoplasia"},{"key":"ref_27","unstructured":"(2001). Recommendations for cryopreservation of cells tumor tissues to be used for molecular analyses. Ann. Pathol., 21, 184\u2013201."},{"key":"ref_28","unstructured":"Le Comit\u00e9 consultatif national d\u2019\u00e9thique pour les sciences de la vie et de la sant\u00e9 (2004). French Bioethics Law, No. 2004-800, July 8th."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1146\/annurev-pathol-011110-130235","article-title":"Molecular Genetics of Colorectal Cancer","volume":"6","author":"Fearon","year":"2011","journal-title":"Annu. Rev. Pathol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1515\/bc-2011-231","article-title":"Kallikrein-related peptidase signaling in colon carcinoma cells: Targeting proteinase-activated receptors","volume":"393","author":"Chung","year":"2012","journal-title":"Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.ygyno.2012.09.001","article-title":"Combined expression of KLK4, KLK5, KLK6, and KLK7 by ovarian cancer cells leads to decreased adhesion and paclitaxel-induced chemoresistance","volume":"127","author":"Loessner","year":"2012","journal-title":"Gynecol. Oncol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1515\/hsz-2017-0339","article-title":"Kallikrein-related peptidase 7 overexpression in melanoma cells modulates cell adhesion leading to a malignant phenotype","volume":"399","author":"Haddada","year":"2018","journal-title":"Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1002\/1878-0261.12103","article-title":"Aberrant expression of kallikrein-related peptidase 7 is correlated with human melanoma aggressiveness by stimulating cell migration and invasion","volume":"11","author":"Delaunay","year":"2017","journal-title":"Mol. Oncol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Tieng, F.Y.F., Abu, N., Sukor, S., Mohd Azman, Z.A., Mahamad Nadzir, N., Lee, L.H., and Mutalib, N.S.A. (2020). L1CAM, CA9, KLK6, HPN, and ALDH1A1 as Potential Serum Markers in Primary and Metastatic Colorectal Cancer Screening. Diagnostics, 10.","DOI":"10.3390\/diagnostics10070444"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1515\/hsz-2014-0166","article-title":"Kallikrein-related peptidase 6 (KLK6) expression in the progression of colon adenoma to carcinoma","volume":"395","author":"Vakrakou","year":"2014","journal-title":"Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1158\/1078-0432.CCR-04-2281","article-title":"Clinical significance of human kallikrein gene 6 messenger RNA expression in colorectal cancer","volume":"11","author":"Ogawa","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.prp.2011.12.010","article-title":"Evaluation and prognostic significance of human tissue kallikrein-related peptidase 6 (KLK6) in colorectal cancer","volume":"208","author":"Petraki","year":"2012","journal-title":"Pathol. Res. Pract."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"17299","DOI":"10.1038\/s41598-017-16269-6","article-title":"Molecular cloning of novel transcripts of human kallikrein-related peptidases 5, 6, 7, 8 and 9 (KLK5\u2013KLK9), using Next-generation sequencing","volume":"7","author":"Adamopoulos","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Candido, J.B., Maiques, O., Boxberg, M., Kast, V., Peerani, E., Tom\u00e1s-Bort, E., Weichert, W., Sananes, A., Papo, N., and Magdolen, V. (2021). Kallikrein-Related Peptidase 6 Is Associated with the Tumour Microenvironment of Pancreatic Ductal Adenocarcinoma. Cancers, 13.","DOI":"10.3390\/cancers13163969"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1515\/BC.2008.087","article-title":"Kallikrein 6 is a mediator of K-RAS-dependent migration of colon carcinoma cells","volume":"389","author":"Henkhaus","year":"2008","journal-title":"Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1534735419864434","DOI":"10.1177\/1534735419864434","article-title":"Identification of Prognostic Biomarkers and Drugs Targeting Them in Colon Adenocarcinoma: A Bioinformatic Analysis","volume":"18","author":"Dong","year":"2019","journal-title":"Integr. Cancer Ther."},{"key":"ref_42","first-page":"1459","article-title":"Let7i5p inhibits the proliferation and metastasis of colon cancer cells by targeting kallikreinrelated peptidase 6","volume":"40","author":"Song","year":"2018","journal-title":"Oncol. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1002\/1878-0261.12493","article-title":"Biochemical pathways mediated by KLK6 protease in breast cancer","volume":"13","author":"Pampalakis","year":"2019","journal-title":"Mol. Oncol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jdermsci.2019.08.007","article-title":"TNF-alpha and IL-17A induce the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes","volume":"96","author":"Sugihara","year":"2019","journal-title":"J. Dermatol. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/1475-2867-13-89","article-title":"Acidic extracellular microenvironment and cancer","volume":"13","author":"Kato","year":"2013","journal-title":"Cancer Cell Int."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1083\/jcb.145.3.551","article-title":"p120(ctn) acts as an inhibitory regulator of cadherin function in colon carcinoma cells","volume":"145","author":"Aono","year":"1999","journal-title":"J. Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1038\/s41598-018-19384-0","article-title":"Exclusion from spheroid formation identifies loss of essential cell\u2013cell adhesion molecules in colon cancer cells","volume":"8","author":"Stadler","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4497","DOI":"10.21873\/anticanres.15260","article-title":"E-Cadherin and Angiopoietin-2 as Potential Biomarkers for Colorectal Cancer With Peritoneal Carcinomatosis","volume":"41","author":"Kim","year":"2021","journal-title":"Anticancer Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s10585-013-9615-4","article-title":"Metastasis of ovarian cancer is mediated by kallikrein related peptidases","volume":"31","author":"Dong","year":"2014","journal-title":"Clin. Exp. Metastasis"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1515\/bc.2010.045","article-title":"Functional proteomics of kallikrein-related peptidases in ovarian cancer ascites fluid","volume":"391","author":"Oikonomopoulou","year":"2010","journal-title":"Biol. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1515\/BC.2008.086","article-title":"Immunofluorometric activity-based probe analysis of active KLK6 in biological fluids","volume":"389","author":"Oikonomopoulou","year":"2008","journal-title":"Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12943-017-0691-y","article-title":"Multi-omics of 34 colorectal cancer cell lines-a resource for biomedical studies","volume":"16","author":"Berg","year":"2017","journal-title":"Mol. Cancer"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1158\/1535-7163.MCT-08-0972","article-title":"KRAS\/BRAF mutation status and ERK1\/2 activation as biomarkers for MEK1\/2 inhibitor therapy in colorectal cancer","volume":"8","author":"Yeh","year":"2009","journal-title":"Mol. Cancer Ther."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"637","DOI":"10.3748\/wjg.v18.i7.637","article-title":"Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array","volume":"18","author":"Abajo","year":"2012","journal-title":"World J. Gastroenterol. 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