{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:52:23Z","timestamp":1790614343313,"version":"4.1.0"},"reference-count":92,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,6]],"date-time":"2022-08-06T00:00:00Z","timestamp":1659744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY029659"],"award-info":[{"award-number":["R01EY029659"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival. We analyzed the impacts of vitamin A deficiency on the morphology, the lipidome, and the proteome of the Drosophila eye. We found that chronic vitamin A deprivation damaged the light-sensing compartments and caused a dramatic loss of visual pigments, but also decreased the molar abundance of most phototransduction proteins that amplify and transduce the visual signal. Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins. In contrast, we found no apparent effects of vitamin A deficiency on the ocular lipidome. In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated.<\/jats:p>","DOI":"10.3390\/biom12081083","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T21:03:50Z","timestamp":1659906230000},"page":"1083","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3578-3506","authenticated-orcid":false,"given":"Mukesh","family":"Kumar","sequence":"first","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstra\u00dfe 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2355-3727","authenticated-orcid":false,"given":"Canan","family":"Has","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstra\u00dfe 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khanh","family":"Lam-Kamath","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Massachusetts Boston, Integrated Sciences Complex, 100 Morrissey Boulevard, Boston, MA 02125, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4813-5900","authenticated-orcid":false,"given":"Sophie","family":"Ayciriex","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstra\u00dfe 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-5268","authenticated-orcid":false,"given":"Deepshe","family":"Dewett","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Massachusetts Boston, Integrated Sciences Complex, 100 Morrissey Boulevard, Boston, MA 02125, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mhamed","family":"Bashir","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Massachusetts Boston, Integrated Sciences Complex, 100 Morrissey Boulevard, Boston, MA 02125, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3145-9773","authenticated-orcid":false,"given":"Clara","family":"Poupault","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Massachusetts Boston, Integrated Sciences Complex, 100 Morrissey Boulevard, Boston, MA 02125, 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