{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T19:15:25Z","timestamp":1789413325825,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"NIDDK Diabetic Complications Consortium","doi-asserted-by":"publisher","award":["DK076169"],"award-info":[{"award-number":["DK076169"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"NIDDK Diabetic Complications Consortium","doi-asserted-by":"publisher","award":["DK115255"],"award-info":[{"award-number":["DK115255"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"NIDDK Diabetic Complications Consortium","doi-asserted-by":"publisher","award":["NHLBI PO1 HL-134604"],"award-info":[{"award-number":["NHLBI PO1 HL-134604"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Aging impairs the expression of HSP70, an emergent player in vascular biology. However, it is unknown if age-related alterations in HSP70 are linked to a decline in arterial function. In this study, we test the hypothesis that the contributions of HSP70 to vascular contraction are diminished in middle-aged animals. We determined the basal levels of HSP70 in the aorta of young and middle-aged Sprague Dawley male rats using Western blotting. Functional studies were performed in a wire myograph system. Force development in response to phenylephrine was assessed in the presence or absence of extracellular calcium (Ca2+), and in aortic rings treated or non-treated with an HSP70 inhibitor. Fluorescent probes were used to evaluate vascular oxidative stress and nitric oxide levels. We report that middle-aged rats have significantly lower levels of HSP70. Blockade of HSP70 attenuated vascular phasic and tonic contraction in isolated aortas. It appears that a functional HSP70 is required for proper Ca2+ handling as inhibition of this protein led to reduced force\u2013displacement in response to Ca2+ dynamics. Furthermore, middle-aged aortic rings exposed to the HSP70 inhibitor display higher reactive oxygen species levels without changes in nitric oxide. In summary, we show that middle-aged animals have lower levels of HSP70 in aortas, which associates with an age-related decline in vascular responses to \u03b1-1 adrenergic stimulation.<\/jats:p>","DOI":"10.3390\/biom12081125","type":"journal-article","created":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T23:44:25Z","timestamp":1660693465000},"page":"1125","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Age-Related Decline in Vascular Responses to Phenylephrine Is Associated with Reduced Levels of HSP70"],"prefix":"10.3390","volume":"12","author":[{"given":"Amanda A.","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Laboratory of Vascular Biology, Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina O.","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Laboratory of Vascular Biology, Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Priviero","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy, Cardiovascular Translational Research Center, University of South Carolina, Columbia, SC 29208, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Clinton","family":"Webb","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy, Cardiovascular Translational Research Center, University of South Carolina, Columbia, SC 29208, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4838-7617","authenticated-orcid":false,"given":"Kenia P.","family":"Nunes","sequence":"additional","affiliation":[{"name":"Laboratory of Vascular Biology, Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1136\/pgmj.2005.036053","article-title":"Ageing and Vascular Ageing","volume":"82","author":"Jani","year":"2006","journal-title":"Postgrad. Med. 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