{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T15:16:28Z","timestamp":1772723788045,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Program for Guangdong Introducing Innovative and Entrepreneurial Teams","award":["2019ZT08Y481"],"award-info":[{"award-number":["2019ZT08Y481"]}]},{"name":"Program for Guangdong Introducing Innovative and Entrepreneurial Teams","award":["ZDSYS20200923172000001"],"award-info":[{"award-number":["ZDSYS20200923172000001"]}]},{"name":"Program for Guangdong Introducing Innovative and Entrepreneurial Teams","award":["JCYJ20210324130408023"],"award-info":[{"award-number":["JCYJ20210324130408023"]}]},{"DOI":"10.13039\/501100017607","name":"Shenzhen Fundamental Research Program","doi-asserted-by":"publisher","award":["2019ZT08Y481"],"award-info":[{"award-number":["2019ZT08Y481"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017607","name":"Shenzhen Fundamental Research Program","doi-asserted-by":"publisher","award":["ZDSYS20200923172000001"],"award-info":[{"award-number":["ZDSYS20200923172000001"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017607","name":"Shenzhen Fundamental Research Program","doi-asserted-by":"publisher","award":["JCYJ20210324130408023"],"award-info":[{"award-number":["JCYJ20210324130408023"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen Fundamental Research Program","award":["2019ZT08Y481"],"award-info":[{"award-number":["2019ZT08Y481"]}]},{"name":"Shenzhen Fundamental Research Program","award":["ZDSYS20200923172000001"],"award-info":[{"award-number":["ZDSYS20200923172000001"]}]},{"name":"Shenzhen Fundamental Research Program","award":["JCYJ20210324130408023"],"award-info":[{"award-number":["JCYJ20210324130408023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Hyperthyroidism is common and can induce cardiomyopathy, but there is no effective therapeutic strategy. The purpose of this study was to investigate the molecular mechanism of hyperthyroidism-induced cardiomyopathy (HTC) and the effect of N-acetylcysteine (NAC), an ROS inhibitor, on the pathophysiology of HTC in vivo and in vitro. Compared with those in the control groups in vivo and in vitro, TT3 and TT4 were significantly increased, the structure of myocardial cells was enlarged and disordered, and interstitial fibrosis and the apoptosis of myocardial cells were markedly increased in the L-Thy group. The ROS and inflammatory response were increased in the hyperthyroidism group. In the NAC group, the contents of TT3 and TT4 were decreased, the myocardial cell structure was slightly disturbed, fibrosis and apoptosis were significantly reduced, and the ROS level and inflammatory response were significantly reduced. Interestingly, L-Thy decreased the viability of fibroblasts and H9c2 cells, suggesting that L-Thy-induced fibrosis was not caused by the proliferation of fibroblasts. The molecular mechanism of HTC could be explained by the fact that L-Thy could cause cardiac hypertrophy, inflammation, and fibrosis by regulating the Ca2+\/calpain\/Rcan1-dependent signalling pathway, the Ca2+\/Rcan1\/NF-\u03baB\/p65-dependent signalling pathway, and the Ca2+\/ROS\/Bcl-2\/caspase-3-dependent signalling pathway. In conclusion, NAC can alleviate the pathophysiology of hyperthyroidism-induced cardiomyopathy, probably by regulating the ROS\/Ca2+-dependent pathway.<\/jats:p>","DOI":"10.3390\/biom12091195","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T21:01:31Z","timestamp":1661806891000},"page":"1195","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["N-Acetylcysteine, an ROS Inhibitor, Alleviates the Pathophysiology of Hyperthyroidism-Induced Cardiomyopathy via the ROS\/Ca2+ Pathway"],"prefix":"10.3390","volume":"12","author":[{"given":"Mengni","family":"Bao","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen 518057, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiumeng","family":"Hua","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences, Peking Union Medical College, 167A Beilishi Road, Xi Cheng District, Beijing 100037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5089-9638","authenticated-orcid":false,"given":"Han","family":"Mo","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen 518057, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Sun","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen 518057, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Thyroid Surgery, Guangzhou First People\u2019s Hospital, School of Medicine, South China University of Technology, Guangzhou 518057, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences, Peking Union Medical College, 167A Beilishi Road, Xi Cheng District, Beijing 100037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengda","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences, Peking Union Medical College, 167A Beilishi Road, Xi Cheng District, Beijing 100037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinjie","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences, Peking Union Medical College, 167A Beilishi Road, Xi Cheng District, Beijing 100037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9488-7823","authenticated-orcid":false,"given":"Jiangping","family":"Song","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen 518057, China"},{"name":"State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences, Peking Union Medical College, 167A Beilishi Road, Xi Cheng District, Beijing 100037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"606931","DOI":"10.3389\/fphys.2021.606931","article-title":"Thyroid Hormone Plays an Important Role in Cardiac Function: From Bench to Bedside","volume":"12","author":"Yamakawa","year":"2021","journal-title":"Front. 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