{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:31:48Z","timestamp":1760243508879,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T00:00:00Z","timestamp":1666915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["SC3GM086249","1827782","0742426"],"award-info":[{"award-number":["SC3GM086249","1827782","0742426"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation (NSF)","doi-asserted-by":"publisher","award":["SC3GM086249","1827782","0742426"],"award-info":[{"award-number":["SC3GM086249","1827782","0742426"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Nora Eccles Treadwell Foundation","award":["SC3GM086249","1827782","0742426"],"award-info":[{"award-number":["SC3GM086249","1827782","0742426"]}]},{"name":"NIH P20CA118783 and the NSF IMPACT LA GK-12 Program","award":["SC3GM086249","1827782","0742426"],"award-info":[{"award-number":["SC3GM086249","1827782","0742426"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Hypothermic (cold) preservation is a limiting factor for successful cell and tissue transplantation where cell swelling (edema) usually develops, impairing cell function. University of Wisconsin (UW) solution, a standard cold preservation solution, contains effective components to suppress hypothermia-induced cell swelling. Antifreeze proteins (AFPs) found in many cold-adapted organisms can prevent cold injury of the organisms. Here, the effects of a beetle AFP from Dendroides canadensis (DAFP-1) on pancreatic \u03b2-cells preservation were first investigated. As low as 500 \u00b5g\/mL, DAFP-1 significantly minimized INS-1 cell swelling and subsequent cell death during 4 \u00b0C preservation in UW solution for up to three days. However, such significant cytoprotection was not observed by an AFP from Tenebrio molitor (TmAFP), a structural homologue to DAFP-1 but lacking arginine, at the same levels. The cytoprotective effect of DAFP-1 was further validated with the primary \u03b2-cells in the isolated rat pancreatic islets in UW solution. The submilligram level supplement of DAFP-1 to UW solution significantly increased the islet mass recovery after three days of cold preservation followed by rewarming. The protective effects of DAFP-1 in UW solution were discussed at a molecular level. The results indicate the potential of DAFP-1 to enhance cell survival during extended cold preservation.<\/jats:p>","DOI":"10.3390\/biom12111584","type":"journal-article","created":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T23:45:00Z","timestamp":1667087100000},"page":"1584","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival"],"prefix":"10.3390","volume":"12","author":[{"given":"Keiko","family":"Omori","sequence":"first","affiliation":[{"name":"Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignacio","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1305-1045","authenticated-orcid":false,"given":"Shanti N.","family":"Raminani","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor M.","family":"Deleon","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Meza","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose","family":"Zamalloa","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel G.","family":"Perez","sequence":"additional","affiliation":[{"name":"Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0876-4809","authenticated-orcid":false,"given":"Hirotake","family":"Komatsu","sequence":"additional","affiliation":[{"name":"Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismail H.","family":"Al-Abdullah","sequence":"additional","affiliation":[{"name":"Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2465-7870","authenticated-orcid":false,"given":"Xin","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1097\/00007890-199002000-00004","article-title":"Important components of the UW solution","volume":"49","author":"Southard","year":"1990","journal-title":"Transplantation"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/0011-2240(86)90056-8","article-title":"Development of a cold storage solution for pancreas preservation","volume":"23","author":"Wahlberg","year":"1986","journal-title":"Cryobiology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3398","DOI":"10.1016\/j.transproceed.2011.09.052","article-title":"Pancreas Preservation for Pancreas and Islet Transplantation: A Minireview","volume":"43","author":"Squifflet","year":"2011","journal-title":"Transplant. 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