{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T03:28:25Z","timestamp":1784258905546,"version":"3.55.0"},"reference-count":75,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["18-04-01250"],"award-info":[{"award-number":["18-04-01250"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["21-15-00123"],"award-info":[{"award-number":["21-15-00123"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["18-04-01250"],"award-info":[{"award-number":["18-04-01250"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-15-00123"],"award-info":[{"award-number":["21-15-00123"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Caveolin-1 is a cholesterol-binding scaffold protein, which is localized in detergent-resistant membrane (DRM) rafts and interacts with components of signal transduction systems, including visual cascade. Among these components are neuronal calcium sensors (NCSs), some of which are redox-sensitive proteins that respond to calcium signals by modulating the activity of multiple intracellular targets. Here, we report that the formation of the caveolin-1 complex with recoverin, a photoreceptor NCS serving as the membrane-binding regulator of rhodopsin kinase (GRK1), is a redox-dependent process. Biochemical and biophysical in vitro experiments revealed a two-fold decreased affinity of recoverin to caveolin-1 mutant Y14E mimicking its oxidative stress-induced phosphorylation of the scaffold protein. At the same time, wild-type caveolin-1 demonstrated a 5\u201310-fold increased affinity to disulfide dimer of recoverin (dRec) or its thiol oxidation mimicking the C39D mutant. The formation of dRec in vitro was not affected by caveolin-1 but was significantly potentiated by zinc, the well-known mediator of redox homeostasis. In the MDCK cell model, oxidative stress indeed triggered Y14 phosphorylation of caveolin-1 and disulfide dimerization of recoverin. Notably, oxidative conditions promoted the accumulation of phosphorylated caveolin-1 in the plasma membrane and the recruitment of recoverin to the same sites. Co-localization of these proteins was preserved upon depletion of intracellular calcium, i.e., under conditions reducing membrane affinity of recoverin but favoring its interaction with caveolin-1. Taken together, these data suggest redox regulation of the signaling complex between recoverin and caveolin-1. During oxidative stress, the high-affinity interaction of thiol-oxidized recoverin with caveolin-1\/DRMs may disturb the light-induced translocation of the former within photoreceptors and affect rhodopsin desensitization.<\/jats:p>","DOI":"10.3390\/biom12111698","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T03:24:34Z","timestamp":1668655474000},"page":"1698","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Redox Regulation of Signaling Complex between Caveolin-1 and Neuronal Calcium Sensor Recoverin"],"prefix":"10.3390","volume":"12","author":[{"given":"Vasiliy I.","family":"Vladimirov","sequence":"first","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita P.","family":"Shchannikova","sequence":"additional","affiliation":[{"name":"Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6220-5341","authenticated-orcid":false,"given":"Alexey V.","family":"Baldin","sequence":"additional","affiliation":[{"name":"Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey S.","family":"Kazakov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina P.","family":"Shevelyova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliya A.","family":"Nazipova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoriia E.","family":"Baksheeva","sequence":"additional","affiliation":[{"name":"Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina L.","family":"Nemashkalova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6980-0028","authenticated-orcid":false,"given":"Anastasia S.","family":"Frolova","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia K.","family":"Tikhomirova","sequence":"additional","affiliation":[{"name":"Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel P.","family":"Philippov","sequence":"additional","affiliation":[{"name":"Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3046-4565","authenticated-orcid":false,"suffix":"Jr.","given":"Andrey A.","family":"Zamyatnin","sequence":"additional","affiliation":[{"name":"Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia"},{"name":"Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow 119991, Russia"},{"name":"Scientific Center for Translation Medicine, Sirius University of Science and Technology, Sochi 354340, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4086-3137","authenticated-orcid":false,"given":"Sergei E.","family":"Permyakov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7181-6283","authenticated-orcid":false,"given":"Dmitry V.","family":"Zinchenko","sequence":"additional","affiliation":[{"name":"Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3013-7863","authenticated-orcid":false,"given":"Evgeni Yu.","family":"Zernii","sequence":"additional","affiliation":[{"name":"Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1152\/physrev.00046.2003","article-title":"Role of caveolae and caveolins in health and 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