{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T22:21:32Z","timestamp":1787869292139,"version":"build-2784847793"},"reference-count":49,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T00:00:00Z","timestamp":1669593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biolab Sanus Farmac\u00eautica"},{"name":"National Council for Scientific and Technological Development, CNPq"},{"name":"CAPES\/PNPD"},{"name":"Research Incentive Fund from Hospital de Cl\u00ednicas de Porto Alegre, FIPE-HCPA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Cardiovascular (CV) disease is the main cause of death in nonalcoholic fatty liver disease (NAFLD), a clinical condition without any approved pharmacological therapy. Thus, we investigated the effects of ornithine aspartate (LOLA) and\/or Vitamin E (VitE) on CV parameters in a steatohepatitis experimental model. Adult Sprague Dawley rats were randomly assigned (10 animals each) and treated from 16 to 28 weeks with gavage as follows: controls (standard diet plus distilled water (DW)), NAFLD (high-fat choline-deficient diet (HFCD) plus DW), NAFLD+LOLA (HFCD plus LOLA (200 mg\/kg\/day)), NAFLD+VitE (HFCD plus VitE (150 mg twice a week)) or NAFLD+LOLA+VitE in the same doses. Atherogenic ratios were higher in NAFLD when compared with NAFLD+LOLA+VitE and controls (p &lt; 0.05). Serum concentration of IL-1\u03b2, IL-6, TNF-\u03b1, MCP-1, e-selectin, ICAM-1, and PAI-1 were not different in intervention groups and controls (p &gt; 0.05). NAFLD+LOLA decreased miR-122, miR-33a, and miR-186 (p &lt; 0.05, for all) in relation to NAFLD. NAFLD+LOLA+VitE decreased miR-122, miR-33a and miR-186, and increased miR-126 (p &lt; 0.05, for all) in comparison to NAFLD and NAFLD+VitE. NAFLD+LOLA and NAFLD+LOLA+VitE prevented liver collagen deposition (p = 0.006) in comparison to NAFLD. Normal cardiac fibers (size and shape) were lower in NAFLD in relation to the others; and the inverse was reported for the percentage of regular hypertrophic cardiomyocytes. NAFLD+LOLA+VitE promoted a significant improvement in atherogenic dyslipidemia, liver fibrosis, and paracrine signaling of lipid metabolism and endothelial dysfunction. This association should be further explored in the treatment of NAFLD-associated CV risk factors.<\/jats:p>","DOI":"10.3390\/biom12121773","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T01:42:02Z","timestamp":1669686122000},"page":"1773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats"],"prefix":"10.3390","volume":"12","author":[{"given":"Laura Bainy Rodrigues","family":"de Freitas","sequence":"first","affiliation":[{"name":"Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, RS, Brazil"},{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4453-7227","authenticated-orcid":false,"given":"Larisse","family":"Longo","sequence":"additional","affiliation":[{"name":"Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, RS, Brazil"},{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Filippi-Chiela","sequence":"additional","affiliation":[{"name":"Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre 91501-970, RS, Brazil"},{"name":"Department of Morphological Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valessa Emanoele Gabriel","family":"de Souza","sequence":"additional","affiliation":[{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6766-734X","authenticated-orcid":false,"given":"Luiza","family":"Behrens","sequence":"additional","affiliation":[{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Henrique Mariano","family":"Pereira","sequence":"additional","affiliation":[{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiza Cec\u00edlia","family":"Leonhard","sequence":"additional","affiliation":[{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulianna","family":"Zanettini","sequence":"additional","affiliation":[{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Eduardo","family":"Pinzon","sequence":"additional","affiliation":[{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto 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RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0526-3067","authenticated-orcid":false,"given":"Carolina","family":"Uribe-Cruz","sequence":"additional","affiliation":[{"name":"Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, RS, Brazil"},{"name":"Experimental Laboratory of Hepatology and Gastroenterology, Center for Experimental Research, Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, RS, Brazil"},{"name":"Medical Genomics and Biotechnology Group, Universidad Cat\u00f3lica de las Misiones, Posadas 3300, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio Reis","family":"\u00c1lvares-da-Silva","sequence":"additional","affiliation":[{"name":"Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, RS, Brazil"},{"name":"Experimental Laboratory of 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