{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T12:06:10Z","timestamp":1772539570613,"version":"3.50.1"},"reference-count":84,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANSES (Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l\u2019Alimentation, de l\u2019Environnement et du travail)","award":["EST-19\u2013025"],"award-info":[{"award-number":["EST-19\u2013025"]}]},{"name":"ANSES (Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l\u2019Alimentation, de l\u2019Environnement et du travail)","award":["ENV202109013715"],"award-info":[{"award-number":["ENV202109013715"]}]},{"name":"ANSES (Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l\u2019Alimentation, de l\u2019Environnement et du travail)","award":["ANR-21-CE44-0022"],"award-info":[{"award-number":["ANR-21-CE44-0022"]}]},{"name":"FRM (Fondation de la Recherche M\u00e9dicale)","award":["EST-19\u2013025"],"award-info":[{"award-number":["EST-19\u2013025"]}]},{"name":"FRM (Fondation de la Recherche M\u00e9dicale)","award":["ENV202109013715"],"award-info":[{"award-number":["ENV202109013715"]}]},{"name":"FRM (Fondation de la Recherche M\u00e9dicale)","award":["ANR-21-CE44-0022"],"award-info":[{"award-number":["ANR-21-CE44-0022"]}]},{"name":"ANR (Agence Nationale de la Recherche, ADTIQ Park)","award":["EST-19\u2013025"],"award-info":[{"award-number":["EST-19\u2013025"]}]},{"name":"ANR (Agence Nationale de la Recherche, ADTIQ Park)","award":["ENV202109013715"],"award-info":[{"award-number":["ENV202109013715"]}]},{"name":"ANR (Agence Nationale de la Recherche, ADTIQ Park)","award":["ANR-21-CE44-0022"],"award-info":[{"award-number":["ANR-21-CE44-0022"]}]},{"name":"Association France Parkinson\u2013subvention 2020","award":["EST-19\u2013025"],"award-info":[{"award-number":["EST-19\u2013025"]}]},{"name":"Association France Parkinson\u2013subvention 2020","award":["ENV202109013715"],"award-info":[{"award-number":["ENV202109013715"]}]},{"name":"Association France Parkinson\u2013subvention 2020","award":["ANR-21-CE44-0022"],"award-info":[{"award-number":["ANR-21-CE44-0022"]}]},{"name":"CNRS (Centre National de Recherche Scientifique)","award":["EST-19\u2013025"],"award-info":[{"award-number":["EST-19\u2013025"]}]},{"name":"CNRS (Centre National de Recherche Scientifique)","award":["ENV202109013715"],"award-info":[{"award-number":["ENV202109013715"]}]},{"name":"CNRS (Centre National de Recherche Scientifique)","award":["ANR-21-CE44-0022"],"award-info":[{"award-number":["ANR-21-CE44-0022"]}]},{"name":"Universit\u00e9 Paris Cit\u00e9","award":["EST-19\u2013025"],"award-info":[{"award-number":["EST-19\u2013025"]}]},{"name":"Universit\u00e9 Paris Cit\u00e9","award":["ENV202109013715"],"award-info":[{"award-number":["ENV202109013715"]}]},{"name":"Universit\u00e9 Paris Cit\u00e9","award":["ANR-21-CE44-0022"],"award-info":[{"award-number":["ANR-21-CE44-0022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>DJ-1 (also called PARK7) is a ubiquitously expressed protein involved in the etiology of Parkinson disease and cancers. At least one of its three cysteine residues is functionally essential, and its oxidation state determines the specific function of the enzyme. DJ-1 was recently reported to be persulfidated in mammalian cell lines, but the implications of this post-translational modification have not yet been analyzed. Here, we report that recombinant DJ-1 is reversibly persulfidated at cysteine 106 by reaction with various sulfane donors and subsequently inhibited. Strikingly, this reaction is orders of magnitude faster than C106 oxidation by H2O2, and persulfidated DJ-1 behaves differently than sulfinylated DJ-1. Both these PTMs most likely play a dedicated role in DJ-1 signaling or protective pathways.<\/jats:p>","DOI":"10.3390\/biom13010027","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T02:36:46Z","timestamp":1671763006000},"page":"27","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Persulfidation of DJ-1: Mechanism and Consequences"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4371-6119","authenticated-orcid":false,"given":"Erwan","family":"Galardon","sequence":"first","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Mathas","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1166-5801","authenticated-orcid":false,"given":"Dominique","family":"Padovani","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0136-8072","authenticated-orcid":false,"given":"Laurent","family":"Le Corre","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle","family":"Poncet","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Dairou","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, Universit\u00e9 Paris Cit\u00e9, F-75006 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1126\/science.1077209","article-title":"Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism","volume":"299","author":"Bonifati","year":"2003","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-981-10-6583-5_8","article-title":"Regulation of Signal Transduction by DJ-1","volume":"1037","author":"Oh","year":"2017","journal-title":"Adv. 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