{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:46:34Z","timestamp":1790631994375,"version":"4.1.0"},"reference-count":67,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T00:00:00Z","timestamp":1680825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Incorporation of dietary peptides has been correlated with decreased presence of skeletal abnormalities in marine larvae. In an attempt to clarify the effect of smaller protein fractions on fish larval and post-larval skeleton, we designed three isoenergetic diets with partial substitution of their protein content with 0% (C), 6% (P6) and 12% (P12) shrimp di- and tripeptides. Experimental diets were tested in zebrafish under two regimes, with inclusion (ADF-Artemia and dry feed) or lack (DF-dry feed only) of live food. Results at the end of metamorphosis highlight the beneficial effect of P12 on growth, survival and early skeletal quality when dry diets are provided from first feeding (DF). Exclusive feeding with P12 also increased the musculoskeletal resistance of the post-larval skeleton against the swimming challenge test (SCT). On the contrary, Artemia inclusion (ADF) overruled any peptide effect in total fish performance. Given the unknown species\u2019 larval nutrient requirements, a 12% dietary peptide incorporation is proposed for successful rearing without live food. A potential nutritional control of the larval and post-larval skeletal development even in aquaculture species is suggested. Limitations of the current molecular analysis are discussed to enable the future identification of the peptide-driven regulatory pathways.<\/jats:p>","DOI":"10.3390\/biom13040659","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T03:26:06Z","timestamp":1681097166000},"page":"659","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Early Peptide Diets on Zebrafish Skeletal Development"],"prefix":"10.3390","volume":"13","author":[{"given":"Alice","family":"Printzi","sequence":"first","affiliation":[{"name":"Biology Department, University of Crete, 70013 Crete, Greece"},{"name":"IFREMER, University of Brest, CNRS, IRD, LEMAR, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9738-0403","authenticated-orcid":false,"given":"George","family":"Koumoundouros","sequence":"additional","affiliation":[{"name":"Biology Department, University of Crete, 70013 Crete, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Fournier","sequence":"additional","affiliation":[{"name":"DianaAqua, Symrise Group, 56250 Elven, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauriane","family":"Madec","sequence":"additional","affiliation":[{"name":"IFREMER, University of Brest, CNRS, IRD, LEMAR, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8436-4773","authenticated-orcid":false,"given":"Jose-Luis","family":"Zambonino-Infante","sequence":"additional","affiliation":[{"name":"IFREMER, University of Brest, CNRS, IRD, LEMAR, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Mazurais","sequence":"additional","affiliation":[{"name":"IFREMER, University of Brest, CNRS, IRD, LEMAR, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S121","DOI":"10.1111\/raq.12016","article-title":"Skeletal Anomalies in Reared European Fish Larvae and Juveniles. 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